Gene description for DCPS
Gene name decapping enzyme, scavenger
Gene symbol DCPS
Other names/aliases DCS1
HINT-5
HINT5
HSL1
HSPC015
Species Homo sapiens
 Database cross references - DCPS
ExoCarta ExoCarta_28960
Vesiclepedia VP_28960
Entrez Gene 28960
HGNC 29812
MIM 610534
UniProt Q96C86  
 DCPS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DCPS
Molecular Function
    RNA 7-methylguanosine cap binding GO:0000340 IBA
    RNA 7-methylguanosine cap binding GO:0000340 IDA
    RNA exonuclease activity GO:0004532 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity GO:0140932 IDA
Biological Process
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    deadenylation-dependent decapping of nuclear-transcribed mRNA GO:0000290 IBA
    mRNA cis splicing, via spliceosome GO:0045292 IDA
    mRNA methylguanosine-cap decapping GO:0110156 IDA
Subcellular Localization
    P-body GO:0000932 IBA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DCPS in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DCPS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHST4  
Two-hybrid Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 HSPA5 3309
Co-fractionation Homo sapiens
4 CA14 23632
Affinity Capture-MS Homo sapiens
5 RAP1GDS1 5910
Co-fractionation Homo sapiens
6 CDC20 991
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
7 PPP1R2 5504
Co-fractionation Homo sapiens
8 MSN 4478
Co-fractionation Homo sapiens
9 GSS 2937
Co-fractionation Homo sapiens
10 XPO1 7514
Co-fractionation Homo sapiens
11 ASNS 440
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
12 GINS3  
Co-fractionation Homo sapiens
13 CAMK1D 57118
Co-fractionation Homo sapiens
14 NAE1 8883
Co-fractionation Homo sapiens
15 PDHA1 5160
Co-fractionation Homo sapiens
16 AP1M1 8907
Co-fractionation Homo sapiens
17 PPP5C 5536
Co-fractionation Homo sapiens
18 DCPS 28960
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 KPNA2 3838
Two-hybrid Homo sapiens
20 CAPNS1 826
Co-fractionation Homo sapiens
21 PNKP 11284
Co-fractionation Homo sapiens
22 DDX58 23586
Affinity Capture-RNA Homo sapiens
23 ECHS1 1892
Co-fractionation Homo sapiens
24 TUFM 7284
Co-fractionation Homo sapiens
25 PDCD10 11235
Co-fractionation Homo sapiens
26 LRPAP1 4043
Co-fractionation Homo sapiens
27 GAPDH 2597
Co-fractionation Homo sapiens
28 GPC5 2262
Two-hybrid Homo sapiens
29 UBA3 9039
Co-fractionation Homo sapiens
30 FLNB 2317
Co-fractionation Homo sapiens
31 DYRK1A 1859
Affinity Capture-MS Homo sapiens
32 NTRK1 4914
Affinity Capture-MS Homo sapiens
33 CSNK2A2 1459
Two-hybrid Homo sapiens
34 PCYT2 5833
Co-fractionation Homo sapiens
35 FZR1  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
36 SERBP1 26135
Affinity Capture-MS Homo sapiens
37 YWHAQ 10971
Reconstituted Complex Homo sapiens
38 AP1G1 164
Co-fractionation Homo sapiens
39 ENO1 2023
Affinity Capture-RNA Homo sapiens
40 KRAS 3845
Negative Genetic Homo sapiens
41 ANAPC4 29945
Co-purification Homo sapiens
42 VCL 7414
Co-fractionation Homo sapiens
43 ACO1 48
Co-fractionation Homo sapiens
44 KPNA5 3841
Two-hybrid Homo sapiens
45 CEBPA  
Protein-peptide Homo sapiens
46 GINS4  
Co-fractionation Homo sapiens
47 CDC27 996
Two-hybrid Homo sapiens
48 PFAS 5198
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which DCPS is involved
PathwayEvidenceSource
Deadenylation-dependent mRNA decay TAS Reactome
Metabolism of RNA TAS Reactome
mRNA decay by 3' to 5' exoribonuclease TAS Reactome





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