Gene description for ASNS
Gene name asparagine synthetase (glutamine-hydrolyzing)
Gene symbol ASNS
Other names/aliases ASNSD
TS11
Species Homo sapiens
 Database cross references - ASNS
ExoCarta ExoCarta_440
Entrez Gene 440
HGNC 753
MIM 108370
UniProt P08243  
 ASNS identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ASNS
Molecular Function
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    cofactor binding GO:0048037 IEA
    asparagine synthase (glutamine-hydrolyzing) activity GO:0004066 IDA
    protein homodimerization activity GO:0042803 IEA
Biological Process
    response to follicle-stimulating hormone GO:0032354 IEA
    cellular response to glucose starvation GO:0042149 IDA
    response to toxic substance GO:0009636 IEA
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    asparagine biosynthetic process GO:0006529 IDA
    response to methotrexate GO:0031427 IEA
    response to light stimulus GO:0009416 IEA
    L-asparagine biosynthetic process GO:0070981 IEA
    positive regulation of mitotic cell cycle GO:0045931 IDA
    cellular amino acid biosynthetic process GO:0008652 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    PERK-mediated unfolded protein response GO:0036499 TAS
    small molecule metabolic process GO:0044281 TAS
    liver development GO:0001889 IEA
    response to amino acid GO:0043200 IEA
    cellular response to hormone stimulus GO:0032870 IEA
    cellular protein metabolic process GO:0044267 TAS
    glutamine metabolic process GO:0006541 IEA
    response to mechanical stimulus GO:0009612 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ASNS in exosomes
1
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASNS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STRN4 29888
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ASNS is involved
PathwayEvidenceSource
Amino acid synthesis and interconversion (transamination) TAS Reactome
ATF4 activates genes TAS Reactome





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