Gene description for UFL1
Gene name UFM1-specific ligase 1
Gene symbol UFL1
Other names/aliases KIAA0776
Maxer
NLBP
RCAD
Species Homo sapiens
 Database cross references - UFL1
ExoCarta ExoCarta_23376
Entrez Gene 23376
HGNC 23039
MIM 613372
UniProt O94874  
 UFL1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for UFL1
Molecular Function
    ligase activity GO:0016874 IEA
    UFM1 transferase activity GO:0071568 IDA
    protein binding GO:0005515 IPI
Biological Process
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    protein ufmylation GO:0071569 IMP
    regulation of protein localization GO:0032880 IMP
    negative regulation of protein ubiquitination GO:0031397 IDA
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IMP
    osteoblast differentiation GO:0001649 IDA
    positive regulation of cell proliferation GO:0008284 IMP
    response to endoplasmic reticulum stress GO:0034976 IDA
    protein K69-linked ufmylation GO:1990592 IDA
Subcellular Localization
    membrane GO:0016020 IDA
    nucleus GO:0005634 IDA
    protein complex GO:0043234 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IEA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified UFL1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UFL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CASP4  
Affinity Capture-MS Homo sapiens
2 CDK5RAP3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which UFL1 is involved
No pathways found





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