Gene description for ABCD3
Gene name ATP-binding cassette, sub-family D (ALD), member 3
Gene symbol ABCD3
Other names/aliases ABC43
CBAS5
PMP70
PXMP1
ZWS2
Species Homo sapiens
 Database cross references - ABCD3
ExoCarta ExoCarta_5825
Vesiclepedia VP_5825
Entrez Gene 5825
HGNC 67
MIM 170995
UniProt P28288  
 ABCD3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ABCD3
Molecular Function
    long-chain fatty acid transmembrane transporter activity GO:0005324 IBA
    long-chain fatty acid transmembrane transporter activity GO:0005324 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IDA
    ATP hydrolysis activity GO:0016887 IDA
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    ATPase-coupled transmembrane transporter activity GO:0042626 IDA
    protein homodimerization activity GO:0042803 IDA
    fatty acyl-CoA hydrolase activity GO:0047617 IDA
    ABC-type transporter activity GO:0140359 IEA
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IDA
    fatty acid biosynthetic process GO:0006633 IMP
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IDA
    fatty acid beta-oxidation GO:0006635 IGI
    bile acid biosynthetic process GO:0006699 ISS
    peroxisome organization GO:0007031 IBA
    peroxisome organization GO:0007031 IDA
    peroxisome organization GO:0007031 IMP
    response to xenobiotic stimulus GO:0009410 IEA
    response to organic cyclic compound GO:0014070 IEA
    bile acid and bile salt transport GO:0015721 ISS
    long-chain fatty acid import into peroxisome GO:0015910 IBA
    long-chain fatty acid import into peroxisome GO:0015910 IMP
    very long-chain fatty acid catabolic process GO:0042760 IBA
    very long-chain fatty acid catabolic process GO:0042760 IGI
    phytanic acid metabolic process GO:1903512 ISS
Subcellular Localization
    mitochondrion GO:0005739 IEA
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal membrane GO:0005778 TAS
    peroxisomal matrix GO:0005782 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ABCD3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ABCD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NET1 10276
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 MTCH1 23787
Proximity Label-MS Homo sapiens
6 VAPA 9218
Affinity Capture-MS Homo sapiens
7 MARCKS 4082
Proximity Label-MS Homo sapiens
8 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ATP6V1C1 528
Co-fractionation Homo sapiens
10 SP1  
Affinity Capture-MS Homo sapiens
11 CTDNEP1 23399
Proximity Label-MS Homo sapiens
12 Chmp4b 75608
Affinity Capture-MS Mus musculus
13 Atp2a2 11938
Affinity Capture-MS Mus musculus
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 CALM1 801
Affinity Capture-MS Homo sapiens
16 NDUFA4 4697
Affinity Capture-MS Homo sapiens
17 PTPN1 5770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
18 Ktn1  
Affinity Capture-MS Mus musculus
19 SOX2  
Affinity Capture-MS Homo sapiens
20 ABCD1 215
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
21 C9orf78 51759
Affinity Capture-MS Homo sapiens
22 FIS1 51024
Proximity Label-MS Homo sapiens
23 DYRK1A 1859
Affinity Capture-MS Homo sapiens
24 KIF23 9493
Affinity Capture-MS Homo sapiens
25 COQ9  
Affinity Capture-MS Homo sapiens
26 TIMMDC1  
Co-fractionation Homo sapiens
27 RAB3B 5865
Proximity Label-MS Homo sapiens
28 FBXW7  
Affinity Capture-MS Homo sapiens
29 PRC1 9055
Affinity Capture-MS Homo sapiens
30 MDC1  
Affinity Capture-MS Homo sapiens
31 KLF15  
Affinity Capture-MS Homo sapiens
32 MARCH5  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 C1qbp 12261
Affinity Capture-MS Mus musculus
34 CALM3 808
Affinity Capture-MS Homo sapiens
35 SURF1  
Co-fractionation Homo sapiens
36 ATP2A3 489
Affinity Capture-MS Homo sapiens
37 EXD2  
Proximity Label-MS Homo sapiens
38 SLC25A46 91137
Proximity Label-MS Homo sapiens
39 NDUFV1 4723
Co-fractionation Homo sapiens
40 TOMM40 10452
Co-fractionation Homo sapiens
41 BRF2  
Affinity Capture-MS Homo sapiens
42 MECP2 4204
Affinity Capture-MS Homo sapiens
43 MAVS 57506
Proximity Label-MS Homo sapiens
44 RAB35 11021
Proximity Label-MS Homo sapiens
45 TFCP2 7024
Affinity Capture-MS Homo sapiens
46 FAF2 23197
Affinity Capture-MS Homo sapiens
47 OBSL1 23363
Affinity Capture-MS Homo sapiens
48 METTL7A 25840
Proximity Label-MS Homo sapiens
49 KIF14 9928
Affinity Capture-MS Homo sapiens
50 MEST 4232
Affinity Capture-MS Homo sapiens
51 DYRK1B  
Affinity Capture-MS Homo sapiens
52 LMAN1 3998
Proximity Label-MS Homo sapiens
53 NUP155 9631
Proximity Label-MS Homo sapiens
54 UNK  
Affinity Capture-RNA Homo sapiens
55 PXMP2  
Proximity Label-MS Homo sapiens
56 CALM2 805
Affinity Capture-MS Homo sapiens
57 TAP1 6890
Co-fractionation Homo sapiens
58 MTCH2 23788
Proximity Label-MS Homo sapiens
59 VAV1 7409
Affinity Capture-MS Homo sapiens
60 NDUFS4 4724
Co-fractionation Homo sapiens
61 COX15 1355
Proximity Label-MS Homo sapiens
62 USP11 8237
Affinity Capture-MS Homo sapiens
63 PELO 53918
Affinity Capture-MS Homo sapiens
64 PARK2  
Affinity Capture-MS Homo sapiens
65 SAE1 10055
Affinity Capture-MS Homo sapiens
66 STOM 2040
Affinity Capture-MS Homo sapiens
67 PSMD14 10213
Affinity Capture-MS Homo sapiens
68 RAB4A 5867
Proximity Label-MS Homo sapiens
69 PTP4A3  
Affinity Capture-MS Homo sapiens
70 CHMP4B 128866
Affinity Capture-MS Homo sapiens
71 PBXIP1 57326
Affinity Capture-MS Homo sapiens
72 YAP1 10413
Affinity Capture-MS Homo sapiens
73 SYNJ2BP 55333
Co-fractionation Homo sapiens
74 NTRK1 4914
Affinity Capture-MS Homo sapiens
75 FAM134A 79137
Affinity Capture-MS Homo sapiens
76 C20orf24  
Affinity Capture-MS Homo sapiens
77 EEF2 1938
Co-fractionation Homo sapiens
78 PEX14 5195
Co-purification Homo sapiens
Proximity Label-MS Homo sapiens
79 DHX9 1660
Co-fractionation Homo sapiens
80 AGPS 8540
Co-fractionation Homo sapiens
81 MLF2 8079
Affinity Capture-MS Homo sapiens
82 RHOT2 89941
Proximity Label-MS Homo sapiens
83 UBA1 7317
Affinity Capture-MS Homo sapiens
84 SEC61B 10952
Proximity Label-MS Homo sapiens
85 RMDN3 55177
Proximity Label-MS Homo sapiens
86 USP32 84669
Affinity Capture-MS Homo sapiens
87 MRPL41 64975
Co-fractionation Homo sapiens
88 CRELD1 78987
Affinity Capture-MS Homo sapiens
89 ANLN 54443
Affinity Capture-MS Homo sapiens
90 NDUFB11 54539
Co-fractionation Homo sapiens
91 SEC63 11231
Affinity Capture-MS Homo sapiens
92 C15orf48  
Affinity Capture-MS Homo sapiens
93 ZBTB2 57621
Affinity Capture-MS Homo sapiens
94 CLN3 1201
Affinity Capture-MS Homo sapiens
95 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
96 RAB5A 5868
Proximity Label-MS Homo sapiens
97 ECT2 1894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 CTNNB1 1499
Co-localization Homo sapiens
99 MYH14 79784
Affinity Capture-MS Homo sapiens
100 Bmpr1a  
Affinity Capture-MS Mus musculus
101 GPR182  
Affinity Capture-MS Homo sapiens
102 GOLT1B 51026
Affinity Capture-MS Homo sapiens
103 Rmdn3  
Affinity Capture-MS Mus musculus
104 HSD3B7 80270
Proximity Label-MS Homo sapiens
105 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 EGFR 1956
Affinity Capture-MS Homo sapiens
107 FKBP8 23770
Proximity Label-MS Homo sapiens
108 ARF6 382
Proximity Label-MS Homo sapiens
109 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
110 RIPK4  
Affinity Capture-MS Homo sapiens
111 Ect2  
Affinity Capture-MS Mus musculus
112 OCIAD1 54940
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
113 NXF1 10482
Affinity Capture-RNA Homo sapiens
114 MAPK6  
Affinity Capture-MS Homo sapiens
115 FBXO6 26270
Affinity Capture-MS Homo sapiens
116 NEFM 4741
Proximity Label-MS Homo sapiens
117 RPA3 6119
Proximity Label-MS Homo sapiens
118 ATP2A1 487
Affinity Capture-MS Homo sapiens
119 RAB9A 9367
Proximity Label-MS Homo sapiens
120 SIRT6  
Affinity Capture-MS Homo sapiens
121 TUFT1  
Two-hybrid Homo sapiens
122 DDRGK1 65992
Affinity Capture-MS Homo sapiens
123 RNF2  
Affinity Capture-MS Homo sapiens
124 HAX1  
Proximity Label-MS Homo sapiens
125 PEX3 8504
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
126 AKAP1 8165
Proximity Label-MS Homo sapiens
127 DOCK3  
Affinity Capture-MS Homo sapiens
128 ACBD5 91452
Proximity Label-MS Homo sapiens
129 ZNF454  
Co-fractionation Homo sapiens
130 HIPK4  
Affinity Capture-MS Homo sapiens
131 KRT18 3875
Proximity Label-MS Homo sapiens
132 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
133 STARD8  
Affinity Capture-MS Homo sapiens
134 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
135 USP50  
Affinity Capture-MS Homo sapiens
136 DOCK2 1794
Affinity Capture-MS Homo sapiens
137 BKRF1  
Affinity Capture-MS
138 ARRDC5  
Affinity Capture-MS Homo sapiens
139 FBXL6  
Affinity Capture-MS Homo sapiens
140 PGRMC1 10857
Affinity Capture-MS Homo sapiens
141 PEX19 5824
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
142 ZNF746  
Affinity Capture-MS Homo sapiens
143 TMEM63B 55362
Affinity Capture-MS Homo sapiens
144 TPTE  
Affinity Capture-MS Homo sapiens
145 PDHA1 5160
Affinity Capture-MS Homo sapiens
146 SOX4  
Proximity Label-MS Homo sapiens
147 SCN3B  
Affinity Capture-MS Homo sapiens
148 MKI67  
Affinity Capture-MS Homo sapiens
149 PSMA1 5682
Two-hybrid Homo sapiens
150 SFXN1 94081
Proximity Label-MS Homo sapiens
151 UFL1 23376
Affinity Capture-MS Homo sapiens
152 NNT 23530
Co-fractionation Homo sapiens
153 MOV10 4343
Affinity Capture-RNA Homo sapiens
154 CDK9 1025
Affinity Capture-MS Homo sapiens
155 ATP2B2 491
Affinity Capture-MS Homo sapiens
156 RAB11A 8766
Proximity Label-MS Homo sapiens
157 KRAS 3845
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
158 RAB5C 5878
Proximity Label-MS Homo sapiens
159 C9orf72  
Affinity Capture-MS Homo sapiens
160 AR 367
Affinity Capture-MS Homo sapiens
161 SP7  
Affinity Capture-MS Homo sapiens
162 RAB2A 5862
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 CUL4A 8451
Affinity Capture-MS Homo sapiens
164 CD274 29126
Affinity Capture-MS Homo sapiens
165 MPV17 4358
Co-fractionation Homo sapiens
View the network image/svg+xml



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