Gene description for ARHGEF16
Gene name Rho guanine nucleotide exchange factor (GEF) 16
Gene symbol ARHGEF16
Other names/aliases GEF16
NBR
Species Homo sapiens
 Database cross references - ARHGEF16
ExoCarta ExoCarta_27237
Vesiclepedia VP_27237
Entrez Gene 27237
HGNC 15515
UniProt Q5VV41  
 ARHGEF16 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ARHGEF16
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    PDZ domain binding GO:0030165 IPI
    receptor tyrosine kinase binding GO:0030971 IPI
    small GTPase binding GO:0031267 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    regulation of actin cytoskeleton organization GO:0032956 IBA
    cell chemotaxis GO:0060326 IMP
    activation of GTPase activity GO:0090630 IDA
    activation of GTPase activity GO:0090630 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGEF16 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGEF16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP1A2 477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MAPK1IP1L 93487
Two-hybrid Homo sapiens
3 DDX6 1656
Co-fractionation Homo sapiens
4 SFN 2810
Affinity Capture-MS Homo sapiens
5 LBR 3930
Affinity Capture-MS Homo sapiens
6 HACD3 51495
Affinity Capture-MS Homo sapiens
7 RIF1  
Affinity Capture-MS Homo sapiens
8 HNRNPH1 3187
Two-hybrid Homo sapiens
9 MECP2 4204
Affinity Capture-MS Homo sapiens
10 RPS27A 6233
Affinity Capture-MS Homo sapiens
11 ACAT1 38
Affinity Capture-MS Homo sapiens
12 ANKFY1 51479
Affinity Capture-MS Homo sapiens
13 HDAC2 3066
Affinity Capture-MS Homo sapiens
14 PLS3 5358
Co-fractionation Homo sapiens
15 FGD6  
Affinity Capture-MS Homo sapiens
16 ALDH9A1 223
Co-fractionation Homo sapiens
17 ADCK3  
Affinity Capture-MS Homo sapiens
18 ELMO1 9844
Affinity Capture-Western Homo sapiens
19 GAR1 54433
Affinity Capture-MS Homo sapiens
20 HSPA8 3312
Affinity Capture-MS Homo sapiens
21 SNTB2 6645
Affinity Capture-MS Homo sapiens
22 YWHAB 7529
Affinity Capture-MS Homo sapiens
23 SF3A1 10291
Affinity Capture-MS Homo sapiens
24 MGA  
Affinity Capture-MS Homo sapiens
25 EPB41L3 23136
Affinity Capture-MS Homo sapiens
26 XPO1 7514
Affinity Capture-MS Homo sapiens
27 MRPL53  
Affinity Capture-MS Homo sapiens
28 DLG1 1739
Affinity Capture-MS Homo sapiens
29 DPP8 54878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PPIB 5479
Affinity Capture-MS Homo sapiens
31 NUDT21 11051
Affinity Capture-MS Homo sapiens
32 IFT122 55764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 STIP1 10963
Affinity Capture-MS Homo sapiens
34 UTRN 7402
Affinity Capture-MS Homo sapiens
35 YWHAZ 7534
Affinity Capture-MS Homo sapiens
36 TAB1 10454
Affinity Capture-MS Homo sapiens
37 GAPDHS 26330
Affinity Capture-MS Homo sapiens
38 KPNA2 3838
Affinity Capture-MS Homo sapiens
39 SHMT2 6472
Affinity Capture-MS Homo sapiens
40 PDHA1 5160
Affinity Capture-MS Homo sapiens
41 TFG 10342
Two-hybrid Homo sapiens
42 MAPRE1 22919
Proximity Label-MS Homo sapiens
43 ATP5O 539
Affinity Capture-MS Homo sapiens
44 PRKCI 5584
Affinity Capture-MS Homo sapiens
45 RABL3 285282
Affinity Capture-MS Homo sapiens
46 RBFOX2 23543
Affinity Capture-MS Homo sapiens
47 SNRNP70 6625
Affinity Capture-MS Homo sapiens
48 MAGED1 9500
Two-hybrid Homo sapiens
49 CDH1 999
Proximity Label-MS Homo sapiens
50 HMGN1  
Affinity Capture-MS Homo sapiens
51 PPIL4  
Affinity Capture-MS Homo sapiens
52 TMEM160 54958
Affinity Capture-MS Homo sapiens
53 LIN7C 55327
Affinity Capture-MS Homo sapiens
54 YWHAH 7533
Affinity Capture-MS Homo sapiens
55 RPL22L1 200916
Affinity Capture-MS Homo sapiens
56 CHCHD2  
Affinity Capture-MS Homo sapiens
57 EPHA2 1969
Affinity Capture-Western Homo sapiens
58 YWHAQ 10971
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
59 SCRIB 23513
Affinity Capture-MS Homo sapiens
60 PML 5371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RHOB 388
Proximity Label-MS Homo sapiens
62 ABCD3 5825
Affinity Capture-MS Homo sapiens
63 YWHAG 7532
Affinity Capture-MS Homo sapiens
64 AKT1 207
Biochemical Activity Homo sapiens
65 LYPLA1 10434
Affinity Capture-MS Homo sapiens
66 DLAT 1737
Affinity Capture-MS Homo sapiens
67 UBAC1 10422
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 HMGN3  
Affinity Capture-MS Homo sapiens
69 TOM1L1 10040
Co-fractionation Homo sapiens
70 LNX1  
Two-hybrid Homo sapiens
71 QPCTL 54814
Affinity Capture-MS Homo sapiens
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