Gene description for NUDT21
Gene name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Gene symbol NUDT21
Other names/aliases CFIM25
CPSF5
Species Homo sapiens
 Database cross references - NUDT21
ExoCarta ExoCarta_11051
Entrez Gene 11051
HGNC 13870
MIM 604978
UniProt O43809  
 NUDT21 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for NUDT21
Molecular Function
    AU-rich element binding GO:0017091 IDA
    RNA binding GO:0003723 IDA
    histone deacetylase binding GO:0042826 IPI
    hydrolase activity GO:0016787 IEA
    protein homodimerization activity GO:0042803 IPI
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    mRNA binding GO:0003729 IDA
Biological Process
    gene expression GO:0010467 TAS
    RNA splicing GO:0008380 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    protein tetramerization GO:0051262 IDA
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA processing GO:0006397 IDA
    mRNA polyadenylation GO:0006378 IMP
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA 3'-end processing GO:0031124 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    centrosome GO:0005813 IDA
    nucleoplasm GO:0005654 TAS
    paraspeckles GO:0042382 IDA
    mRNA cleavage factor complex GO:0005849 IDA
 Experiment description of studies that identified NUDT21 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for NUDT21
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMF1  
Two-hybrid Homo sapiens
2 DKFZP564O0523  
Two-hybrid Homo sapiens
3 CD2BP2 10421
Affinity Capture-MS Homo sapiens
4 CPSF7 79869
Two-hybrid Homo sapiens
5 EXOSC6 118460
Two-hybrid Homo sapiens
6 NUDT21 11051
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 SNRNP70 6625
Reconstituted Complex Homo sapiens
8 RNPS1 10921
Affinity Capture-MS Homo sapiens
9 RPLP1 6176
Two-hybrid Homo sapiens
10 VCL 7414
Affinity Capture-MS Homo sapiens
11 NIF3L1 60491
Two-hybrid Homo sapiens
12 CPSF6 11052
Two-hybrid Homo sapiens
13 MYL6 4637
Two-hybrid Homo sapiens
14 KIAA1377  
Two-hybrid Homo sapiens
View the network image/svg+xml



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