Gene description for SMURF1
Gene name SMAD specific E3 ubiquitin protein ligase 1
Gene symbol SMURF1
Other names/aliases -
Species Homo sapiens
 Database cross references - SMURF1
ExoCarta ExoCarta_57154
Vesiclepedia VP_57154
Entrez Gene 57154
HGNC 16807
MIM 605568
UniProt Q9HCE7  
 SMURF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for SMURF1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin protein ligase activity GO:0061630 TAS
    I-SMAD binding GO:0070411 IPI
    R-SMAD binding GO:0070412 IPI
    activin binding GO:0048185 NAS
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 TAS
    ligase activity GO:0016874 IEA
Biological Process
    protein targeting to vacuole involved in autophagy GO:0071211 IMP
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IDA
    ubiquitin-dependent SMAD protein catabolic process GO:0030579 IDA
    protein polyubiquitination GO:0000209 IDA
    positive regulation of defense response to virus by host GO:0002230 IMP
    negative regulation of ossification GO:0030279 IEA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 TAS
    protein localization to cell surface GO:0034394 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    ectoderm development GO:0007398 TAS
    receptor catabolic process GO:0032801 IDA
    cell differentiation GO:0030154 IDA
    protein ubiquitination GO:0016567 IDA
    positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000060 IMP
    positive regulation of dendrite extension GO:1903861 IDA
    BMP signaling pathway GO:0030509 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    protein export from nucleus GO:0006611 IDA
    protein targeting to plasma membrane GO:0072661 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    negative regulation of BMP signaling pathway GO:0030514 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    neuronal cell body GO:0043025 IEA
    extracellular exosome GO:0070062 IDA
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 IGI
    plasma membrane GO:0005886 IDA
    axon GO:0030424 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SMURF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SMURF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Affinity Capture-Luminescence Mus musculus
2 Rnu3ip2  
Affinity Capture-Luminescence Mus musculus
3 Wee1  
Affinity Capture-Luminescence Mus musculus
4 Sfrs4  
Affinity Capture-Luminescence Mus musculus
5 Ppp2r2d  
Affinity Capture-Luminescence Mus musculus
6 Nola1  
Affinity Capture-Luminescence Mus musculus
7 Smad6  
Affinity Capture-Luminescence Mus musculus
8 Ubb 22187
Affinity Capture-Luminescence Mus musculus
9 Srsf5 20384
Affinity Capture-Luminescence Mus musculus
10 SMAD7  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
11 Arhgef1 16801
Affinity Capture-Luminescence Mus musculus
12 Arhgef11  
Affinity Capture-Luminescence Mus musculus
13 Usp45  
Affinity Capture-Luminescence Mus musculus
14 XPO1 7514
Invitro Homo sapiens
Invivo Homo sapiens
15 Rit1  
Affinity Capture-Luminescence Mus musculus
16 Zbtb8  
Affinity Capture-Luminescence Mus musculus
17 Pak1ip1  
Affinity Capture-Luminescence Mus musculus
18 Strap 20901
Affinity Capture-Luminescence Mus musculus
19 Fbxo3  
Affinity Capture-Luminescence Mus musculus
20 Uhmk1  
Affinity Capture-Luminescence Mus musculus
21 Dnajc7 56354
Affinity Capture-Luminescence Mus musculus
22 Rhpn2  
Affinity Capture-Luminescence Mus musculus
23 Smad3  
Affinity Capture-Luminescence Mus musculus
24 Stub1 56424
Affinity Capture-Luminescence Mus musculus
25 Ap2b1 71770
Affinity Capture-Luminescence Mus musculus
26 Ash2l  
Affinity Capture-Luminescence Mus musculus
27 Rgl3  
Affinity Capture-Luminescence Mus musculus
28 Tssk5  
Affinity Capture-Luminescence Mus musculus
29 Rtkn  
Affinity Capture-Luminescence Mus musculus
30 Anapc5 59008
Affinity Capture-Luminescence Mus musculus
31 Ripk2  
Affinity Capture-Luminescence Mus musculus
32 Fbxo24  
Affinity Capture-Luminescence Mus musculus
33 Rnf14  
Affinity Capture-Luminescence Mus musculus
34 Sf3a1 67465
Affinity Capture-Luminescence Mus musculus
35 Trim35  
Affinity Capture-Luminescence Mus musculus
36 N/A  
Affinity Capture-Luminescence Mus musculus
37 Arhgap15  
Affinity Capture-Luminescence Mus musculus
38 Ubtf 21429
Affinity Capture-Luminescence Mus musculus
39 ARL4D  
Affinity Capture-Luminescence Mus musculus
40 Rnf138  
Affinity Capture-Luminescence Mus musculus
41 Stat1 20846
Affinity Capture-Luminescence Mus musculus
42 2310058A11Rik  
Affinity Capture-Luminescence Mus musculus
43 TGFBR1 7046
Affinity Capture-Western Homo sapiens
44 Cul5 75717
Affinity Capture-Luminescence Mus musculus
45 Tbl2  
Affinity Capture-Luminescence Mus musculus
46 Fcho1  
Affinity Capture-Luminescence Mus musculus
47 Rnf141  
Affinity Capture-Luminescence Mus musculus
48 Lhx9  
Affinity Capture-Luminescence Mus musculus
49 Rhobtb1  
Affinity Capture-Luminescence Mus musculus
50 Cdc40  
Affinity Capture-Luminescence Mus musculus
51 Wdr61  
Affinity Capture-Luminescence Mus musculus
52 Inpp5b  
Affinity Capture-Luminescence Mus musculus
53 Smad7  
Affinity Capture-Luminescence Mus musculus
54 LONRF3  
Affinity Capture-Luminescence Mus musculus
55 Map2k3  
Affinity Capture-Luminescence Mus musculus
56 Arhgef7  
Affinity Capture-Luminescence Mus musculus
57 Ocln  
Affinity Capture-Luminescence Mus musculus
58 Aven  
Affinity Capture-Luminescence Mus musculus
59 Map3k7  
Affinity Capture-Luminescence Mus musculus
60 Sqstm1  
Affinity Capture-Luminescence Mus musculus
61 Rasd2  
Affinity Capture-Luminescence Mus musculus
62 RLIM  
Affinity Capture-Luminescence Mus musculus
63 SMAD6  
Invivo Homo sapiens
64 Pftk1  
Affinity Capture-Luminescence Mus musculus
65 Cxxc1  
Affinity Capture-Luminescence Mus musculus
66 Rapsn  
Affinity Capture-Luminescence Mus musculus
67 Apbb2  
Affinity Capture-Luminescence Mus musculus
68 Ppp1cb 19046
Affinity Capture-Luminescence Mus musculus
69 Csnk2a2 13000
Affinity Capture-Luminescence Mus musculus
70 Osbpl3  
Affinity Capture-Luminescence Mus musculus
71 Cdkl1  
Affinity Capture-Luminescence Mus musculus
72 Gng11  
Affinity Capture-Luminescence Mus musculus
73 NAA20  
Affinity Capture-Luminescence Mus musculus
74 Ing1l  
Affinity Capture-Luminescence Mus musculus
75 Irak2  
Affinity Capture-Luminescence Mus musculus
76 Etv6  
Affinity Capture-Luminescence Mus musculus
77 Elp3 74195
Affinity Capture-Luminescence Mus musculus
78 Hdgfrp3  
Affinity Capture-Luminescence Mus musculus
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