Gene description for TRAF4
Gene name TNF receptor-associated factor 4
Gene symbol TRAF4
Other names/aliases CART1
MLN62
RNF83
Species Homo sapiens
 Database cross references - TRAF4
ExoCarta ExoCarta_9618
Vesiclepedia VP_9618
Entrez Gene 9618
HGNC 12034
MIM 602464
UniProt Q9BUZ4  
 TRAF4 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TRAF4
Molecular Function
    tumor necrosis factor receptor binding GO:0005164 IPI
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    transferase activity GO:0016740 IEA
    protein kinase binding GO:0019901 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    thioesterase binding GO:0031996 IPI
    signaling adaptor activity GO:0035591 IBA
    identical protein binding GO:0042802 IPI
    WW domain binding GO:0050699 IPI
Biological Process
    apoptotic process GO:0006915 IEA
    cell surface receptor signaling pathway GO:0007166 IBA
    respiratory gaseous exchange by respiratory system GO:0007585 IEA
    respiratory tube development GO:0030323 IEA
    regulation of apoptotic process GO:0042981 IEA
    regulation of canonical NF-kappaB signal transduction GO:0043122 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    innate immune response GO:0045087 IEA
    positive regulation of protein kinase activity GO:0045860 IDA
    positive regulation of JNK cascade GO:0046330 IDA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IEA
    bicellular tight junction GO:0005923 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    plasma membrane signaling receptor complex GO:0098802 IBA
 Experiment description of studies that identified TRAF4 in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRAF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 BAHD1  
Two-hybrid Homo sapiens
3 KRT37 8688
Affinity Capture-MS Homo sapiens
4 PKD1P1 339044
Two-hybrid Homo sapiens
5 UBL4A 8266
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 QARS 5859
Two-hybrid Homo sapiens
7 CENPE 1062
Two-hybrid Homo sapiens
8 TRAF4 9618
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 NOS1AP  
Two-hybrid Homo sapiens
10 Ndufa13  
Two-hybrid Mus musculus
11 BYSL 705
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 RPL14 9045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NCF1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
14 ZNF3  
Two-hybrid Homo sapiens
15 TGFBR1 7046
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 MAP3K5 4217
Affinity Capture-Western Homo sapiens
18 DTX3  
Two-hybrid Homo sapiens
19 GP1BB 2812
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 NOD1 10392
Affinity Capture-Western Homo sapiens
21 ISYNA1 51477
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 KRT40  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
23 ZNF512B  
Two-hybrid Homo sapiens
24 CALCOCO2  
Two-hybrid Homo sapiens
25 BCL6B  
Two-hybrid Homo sapiens
26 KRT31 3881
Two-hybrid Homo sapiens
27 BMI1  
Affinity Capture-MS Homo sapiens
28 RIPK2  
Affinity Capture-Western Homo sapiens
29 USP7 7874
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 EXOC7 23265
Two-hybrid Homo sapiens
31 CTAGE5 4253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
33 Ctnnb1 12387
Two-hybrid Mus musculus
34 TNFRSF4  
Affinity Capture-Western Homo sapiens
35 WWP1 11059
Two-hybrid Homo sapiens
36 LTBR 4055
Reconstituted Complex Homo sapiens
37 BANP  
Two-hybrid Homo sapiens
38 GPRASP1  
Two-hybrid Homo sapiens
39 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
40 USP10 9100
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
41 IRAK1  
Affinity Capture-Western Homo sapiens
42 SPDL1  
Two-hybrid Homo sapiens
43 NOD2 64127
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 CBL 867
Two-hybrid Homo sapiens
45 RPS6KB1 6198
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 PFDN2 5202
Affinity Capture-MS Homo sapiens
47 MYH9 4627
Affinity Capture-MS Homo sapiens
48 NGFR 4804
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
49 OGT 8473
Reconstituted Complex Homo sapiens
50 GORASP2 26003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 PTK2B 2185
Affinity Capture-Western Homo sapiens
52 CHEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
53 KIF11 3832
Affinity Capture-MS Homo sapiens
54 CATSPER4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ZNF581  
Two-hybrid Homo sapiens
56 TYK2 7297
Two-hybrid Homo sapiens
57 CD40 958
Affinity Capture-Western Homo sapiens
58 NTRK1 4914
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
59 USP15 9958
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 FAT1 2195
Two-hybrid Homo sapiens
61 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 ANXA1 301
Protein-peptide Homo sapiens
63 TRAF6 7189
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Co-localization Homo sapiens
64 CUL3 8452
Affinity Capture-MS Homo sapiens
65 FAM90A1  
Two-hybrid Homo sapiens
66 TGFB1I1 7041
Affinity Capture-Western Homo sapiens
67 FTH1 2495
Two-hybrid Homo sapiens
68 MAP3K7  
Affinity Capture-Western Homo sapiens
69 HIP1R 9026
Co-fractionation Homo sapiens
70 SUZ12  
Affinity Capture-MS Homo sapiens
71 NPAS2  
Two-hybrid Homo sapiens
72 MAGEC2  
Two-hybrid Homo sapiens
73 PRMT5 10419
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
74 Aacs 78894
Two-hybrid Mus musculus
75 ALKBH4  
Two-hybrid Homo sapiens
76 ING5  
Affinity Capture-MS Homo sapiens
77 ARNT2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 MAP3K4 4216
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 Acat1 110446
Two-hybrid Mus musculus
80 BEND3  
Affinity Capture-MS Homo sapiens
81 RNF114 55905
Two-hybrid Homo sapiens
82 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 REXO1L6P  
Two-hybrid Homo sapiens
84 CHUK 1147
Biochemical Activity Homo sapiens
85 DOCK3  
Affinity Capture-MS Homo sapiens
86 TICAM1  
Affinity Capture-Western Homo sapiens
87 SUMO1 7341
Two-hybrid Homo sapiens
88 UBE2D1 7321
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
89 CBLC  
Two-hybrid Homo sapiens
90 TP53BP2  
Two-hybrid Homo sapiens
91 TRAF2 7186
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
92 TGFBR2 7048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
93 UBE2O 63893
Affinity Capture-Western Homo sapiens
94 HOMEZ  
Two-hybrid Homo sapiens
95 TBC1D7  
Two-hybrid Homo sapiens
96 WBP11  
Two-hybrid Homo sapiens
97 UBL4B  
Two-hybrid Homo sapiens
98 SMURF1 57154
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
99 KANSL1  
Two-hybrid Homo sapiens
100 SALL1 6299
Affinity Capture-MS Homo sapiens
101 NCF2 4688
Affinity Capture-Western Homo sapiens
102 WWP2 11060
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
103 TRIM37  
Reconstituted Complex Homo sapiens
104 KDM1A 23028
Two-hybrid Homo sapiens
105 TNFRSF9  
Affinity Capture-Western Homo sapiens
106 FBXL18 80028
Two-hybrid Homo sapiens
107 Sardh  
Two-hybrid Mus musculus
108 PDE4DIP 9659
Two-hybrid Homo sapiens
109 FAM214B  
Two-hybrid Homo sapiens
110 AR 367
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
111 SMURF2 64750
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
112 GP6 51206
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
113 MRPL28 10573
Two-hybrid Homo sapiens
114 IRS1 3667
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
115 ATOX1 475
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TRAF4 is involved
No pathways found





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