Gene description for TRAF4
Gene name TNF receptor-associated factor 4
Gene symbol TRAF4
Other names/aliases CART1
MLN62
RNF83
Species Homo sapiens
 Database cross references - TRAF4
ExoCarta ExoCarta_9618
Vesiclepedia VP_9618
Entrez Gene 9618
HGNC 12034
MIM 602464
UniProt Q9BUZ4  
 TRAF4 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TRAF4
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IPI
    tumor necrosis factor receptor binding GO:0005164 IPI
    DNA binding GO:0003677 TAS
    protein binding GO:0005515 IPI
    thioesterase binding GO:0031996 IPI
    ubiquitin-protein transferase activity GO:0004842 IEA
    WW domain binding GO:0050699 IPI
    protein kinase binding GO:0019901 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    positive regulation of protein homodimerization activity GO:0090073 IDA
    positive regulation of protein kinase activity GO:0045860 IDA
    respiratory tube development GO:0030323 IEA
    apoptotic process GO:0006915 IEA
    respiratory gaseous exchange GO:0007585 IEA
    activation of NF-kappaB-inducing kinase activity GO:0007250 IEA
    regulation of apoptotic process GO:0042981 IEA
    signal transduction GO:0007165 TAS
    positive regulation of JNK cascade GO:0046330 IDA
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    bicellular tight junction GO:0005923 IEA
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IEA
    nucleus GO:0005634 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified TRAF4 in exosomes
1
Experiment ID 363
MISEV standards
EV Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
DCLK1
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
EV Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
DCLK1
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
EV Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
DCLK1
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 211
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
EV Biophysical techniques
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRAF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPS6KB1 6198
Affinity Capture-Western Homo sapiens
2 TRIM37  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 UBE2I 7329
Affinity Capture-MS Homo sapiens
4 ARGBP2  
Affinity Capture-MS Homo sapiens
5 USP7 7874
Invitro Homo sapiens
6 PLSCR1 5359
Affinity Capture-MS Homo sapiens
7 BCKDK 10295
Affinity Capture-MS Homo sapiens
8 NGFR 4804
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 TGFB1I1 7041
Affinity Capture-MS Homo sapiens
10 TARBP2  
Affinity Capture-MS Homo sapiens
11 NCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 LTBR 4055
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which TRAF4 is involved
No pathways found





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