Gene description for UBE2O
Gene name ubiquitin-conjugating enzyme E2O
Gene symbol UBE2O
Other names/aliases E2-230K
Species Homo sapiens
 Database cross references - UBE2O
ExoCarta ExoCarta_63893
Entrez Gene 63893
HGNC 29554
UniProt Q9C0C9  
 UBE2O identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for UBE2O
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    ATP binding GO:0005524 IEA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    protein monoubiquitination GO:0006513 IDA
    protein K63-linked ubiquitination GO:0070534 IMP
    retrograde transport, endosome to Golgi GO:0042147 IMP
    positive regulation of BMP signaling pathway GO:0030513 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified UBE2O in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBE2O
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which UBE2O is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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