Gene description for PLD1
Gene name phospholipase D1, phosphatidylcholine-specific
Gene symbol PLD1
Other names/aliases -
Species Homo sapiens
 Database cross references - PLD1
ExoCarta ExoCarta_5337
Vesiclepedia VP_5337
Entrez Gene 5337
HGNC 9067
MIM 602382
UniProt Q13393  
 PLD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PLD1
Molecular Function
    phospholipase D activity GO:0004630 IEA
    phosphatidylinositol binding GO:0035091 IEA
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 IEA
    protein binding GO:0005515 IPI
Biological Process
    defense response to Gram-positive bacterium GO:0050830 IEA
    inositol lipid-mediated signaling GO:0048017 IEA
    glycerophospholipid biosynthetic process GO:0046474 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    lipid catabolic process GO:0016042 IEA
    phospholipid metabolic process GO:0006644 TAS
    small molecule metabolic process GO:0044281 TAS
    regulation of cell migration GO:0030334 IEA
    phosphatidic acid biosynthetic process GO:0006654 TAS
    regulation of microvillus assembly GO:0032534 IMP
    chemotaxis GO:0006935 TAS
    Ras protein signal transduction GO:0007265 TAS
    phosphatidylglycerol biosynthetic process GO:0006655 TAS
    regulation of phagocytosis GO:0050764 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    Golgi membrane GO:0000139 IEA
    lysosomal membrane GO:0005765 IDA
    membrane GO:0016020 IDA
    endosome GO:0005768 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    apical plasma membrane GO:0016324 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    late endosome membrane GO:0031902 IEA
    endocytic vesicle GO:0030139 IDA
 Experiment description of studies that identified PLD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIP5K1A 8394
Invivo Homo sapiens
2 PKN1 5585
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 CDC42 998
Invitro Homo sapiens
Invivo Homo sapiens
4 AMPH 273
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
5 SNCA 6622
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 Actb 81822
Reconstituted Complex Rattus norvegicus
7 SRC 6714
Biochemical Activity Homo sapiens
8 RAC1 5879
Invivo Homo sapiens
9 RALA 5898
Reconstituted Complex Homo sapiens
10 RHOA 387
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 PEA15 8682
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 ARF6 382
Invivo Homo sapiens
13 BIN1 274
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
14 PRKCA 5578
Affinity Capture-Western Homo sapiens
15 ARF1 375
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PLD1 is involved
PathwayEvidenceSource
Role of phospholipids in phagocytosis TAS Reactome
Synthesis of PA TAS Reactome
Synthesis of PG TAS Reactome





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