Gene description for PLD1
Gene name phospholipase D1, phosphatidylcholine-specific
Gene symbol PLD1
Other names/aliases -
Species Homo sapiens
 Database cross references - PLD1
ExoCarta ExoCarta_5337
Vesiclepedia VP_5337
Entrez Gene 5337
HGNC 9067
MIM 602382
UniProt Q13393  
 PLD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PLD1
Molecular Function
    phospholipase D activity GO:0004630 IBA
    phospholipase D activity GO:0004630 IDA
    phospholipase D activity GO:0004630 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 TAS
    chemotaxis GO:0006935 TAS
    small GTPase-mediated signal transduction GO:0007264 TAS
    Ras protein signal transduction GO:0007265 TAS
    phospholipid catabolic process GO:0009395 IBA
    cellular response to nutrient GO:0031670 IDA
    regulation of microvillus assembly GO:0032534 IBA
    regulation of microvillus assembly GO:0032534 IMP
    positive regulation of translation GO:0045727 IMP
    regulation of vesicle-mediated transport GO:0060627 IBA
    regulation of synaptic vesicle cycle GO:0098693 IDA
    regulation of synaptic vesicle cycle GO:0098693 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    apical plasma membrane GO:0016324 IDA
    endocytic vesicle GO:0030139 IDA
    cytoplasmic vesicle GO:0031410 IBA
    late endosome membrane GO:0031902 IEA
    specific granule membrane GO:0035579 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    tertiary granule membrane GO:0070821 TAS
    cholinergic synapse GO:0098981 IDA
    cholinergic synapse GO:0098981 IMP
 Experiment description of studies that identified PLD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 CACNG5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MARCKS 4082
Proximity Label-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 AKAP11 11215
Affinity Capture-MS Homo sapiens
9 LTBP2 4053
Affinity Capture-MS Homo sapiens
10 LAMP2 3920
Proximity Label-MS Homo sapiens
11 EGLN1 54583
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 VHL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CFL1 1072
Affinity Capture-Western Homo sapiens
15 FAXC  
Affinity Capture-MS Homo sapiens
16 POLH  
Affinity Capture-MS Homo sapiens
17 RHEB 6009
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
18 TEX264 51368
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 HEATR3 55027
Affinity Capture-MS Homo sapiens
20 PLCG2 5336
Affinity Capture-Western Homo sapiens
21 ANKMY2 57037
Affinity Capture-Western Homo sapiens
22 UBE2O 63893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ING3  
Affinity Capture-MS Homo sapiens
24 SNX27 81609
Affinity Capture-MS Homo sapiens
25 SRC 6714
Biochemical Activity Homo sapiens
26 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SLC31A1 1317
Affinity Capture-MS Homo sapiens
28 RHOA 387
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 PEA15 8682
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
30 ZSWIM8 23053
Affinity Capture-MS Homo sapiens
31 Klc2  
Affinity Capture-MS Mus musculus
32 CEP135  
Affinity Capture-MS Homo sapiens
33 RAB35 11021
Proximity Label-MS Homo sapiens
34 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
35 KCNA4  
Affinity Capture-MS Homo sapiens
36 KIF14 9928
Affinity Capture-MS Homo sapiens
37 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 C15orf59  
Affinity Capture-MS Homo sapiens
39 C1orf186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 AP3M1 26985
Affinity Capture-MS Homo sapiens
41 Actb 81822
Reconstituted Complex Rattus norvegicus
42 MCC 4163
Affinity Capture-MS Homo sapiens
43 CLSTN1 22883
Affinity Capture-MS Homo sapiens
44 RALA 5898
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
45 EIF2B5 8893
Affinity Capture-MS Homo sapiens
46 LYN 4067
Proximity Label-MS Homo sapiens
47 MKRN2 23609
Affinity Capture-RNA Homo sapiens
48 VPRBP 9730
Affinity Capture-MS Homo sapiens
49 PKN1 5585
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 Coro1c 23790
Affinity Capture-MS Mus musculus
51 AP3B1 8546
Affinity Capture-MS Homo sapiens
52 PRKAR1B  
Affinity Capture-MS Homo sapiens
53 DNAJB5  
Affinity Capture-MS Homo sapiens
54 PPM1G 5496
Affinity Capture-MS Homo sapiens
55 ACTG1 71
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
56 BIN1 274
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
57 PIPSL 266971
Affinity Capture-MS Homo sapiens
58 ANLN 54443
Affinity Capture-MS Homo sapiens
59 PER2  
Affinity Capture-MS Homo sapiens
60 CXCR4 7852
Affinity Capture-MS Homo sapiens
61 PRKCA 5578
Affinity Capture-Western Homo sapiens
62 AHCYL1 10768
Affinity Capture-MS Homo sapiens
63 APBA2 321
Affinity Capture-MS Homo sapiens
64 LAMP1 3916
Proximity Label-MS Homo sapiens
65 ASIC2  
Affinity Capture-MS Homo sapiens
66 CD6 923
Affinity Capture-MS Homo sapiens
67 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
68 AP3B2 8120
Affinity Capture-MS Homo sapiens
69 ARF6 382
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
70 EIF2B3 8891
Affinity Capture-MS Homo sapiens
71 TMEM63C  
Affinity Capture-MS Homo sapiens
72 SPACA1 81833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 CCDC85A  
Affinity Capture-MS Homo sapiens
74 CDC42 998
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
75 AMPH 273
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
76 Hsph1 15505
Affinity Capture-MS Mus musculus
77 ZC3H18  
Affinity Capture-MS Homo sapiens
78 RAB9A 9367
Proximity Label-MS Homo sapiens
79 ANKS3  
Affinity Capture-MS Homo sapiens
80 RHOB 388
Proximity Label-MS Homo sapiens
81 MIER2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 TMEM74  
Affinity Capture-MS Homo sapiens
83 BRD3 8019
Affinity Capture-MS Homo sapiens
84 BACH2 60468
Affinity Capture-MS Homo sapiens
85 HIF1A 3091
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
86 GPT 2875
Affinity Capture-MS Homo sapiens
87 FTL 2512
Affinity Capture-MS Homo sapiens
88 SNCA 6622
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
89 LAMTOR1 55004
Proximity Label-MS Homo sapiens
90 ZRANB2 9406
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 TP53 7157
Affinity Capture-MS Homo sapiens
92 RYK 6259
Affinity Capture-MS Homo sapiens
93 PJA2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
94 MAGEA9  
Affinity Capture-MS Homo sapiens
95 SIRT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 PLD3 23646
Affinity Capture-Western Homo sapiens
97 RAB2A 5862
Proximity Label-MS Homo sapiens
98 RAB7A 7879
Proximity Label-MS Homo sapiens
99 ZCCHC10  
Affinity Capture-MS Homo sapiens
100 RAB11A 8766
Proximity Label-MS Homo sapiens
101 ARF1 375
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
102 OSBPL5 114879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 KCTD17 79734
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 PTGES3 10728
Affinity Capture-MS Homo sapiens
105 KRAS 3845
Proximity Label-MS Homo sapiens
106 RAB5C 5878
Proximity Label-MS Homo sapiens
107 LURAP1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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