Gene description for PLD1
Gene name phospholipase D1, phosphatidylcholine-specific
Gene symbol PLD1
Other names/aliases -
Species Homo sapiens
 Database cross references - PLD1
ExoCarta ExoCarta_5337
Entrez Gene 5337
HGNC 9067
MIM 602382
UniProt Q13393  
 PLD1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PLD1
Molecular Function
    phospholipase D activity GO:0004630 IEA
    phosphatidylinositol binding GO:0035091 IEA
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 IEA
    protein binding GO:0005515 IPI
Biological Process
    defense response to Gram-positive bacterium GO:0050830 IEA
    inositol lipid-mediated signaling GO:0048017 IEA
    glycerophospholipid biosynthetic process GO:0046474 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    lipid catabolic process GO:0016042 IEA
    phospholipid metabolic process GO:0006644 TAS
    small molecule metabolic process GO:0044281 TAS
    regulation of cell migration GO:0030334 IEA
    phosphatidic acid biosynthetic process GO:0006654 TAS
    regulation of microvillus assembly GO:0032534 IMP
    chemotaxis GO:0006935 TAS
    Ras protein signal transduction GO:0007265 TAS
    phosphatidylglycerol biosynthetic process GO:0006655 TAS
    regulation of phagocytosis GO:0050764 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    Golgi membrane GO:0000139 IEA
    lysosomal membrane GO:0005765 IDA
    membrane GO:0016020 IDA
    endosome GO:0005768 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    apical plasma membrane GO:0016324 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    late endosome membrane GO:0031902 IEA
    endocytic vesicle GO:0030139 IDA
 Experiment description of studies that identified PLD1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIP5K1A 8394
Invivo Homo sapiens
2 CDC42 998
Invitro Homo sapiens
Invivo Homo sapiens
3 SNCA 6622
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 PKN1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 SRC 6714
Biochemical Activity Homo sapiens
6 RAC1 5879
Invivo Homo sapiens
7 Actb 81822
Reconstituted Complex Rattus norvegicus
8 PEA15  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 AMPH  
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
10 RALA 5898
Reconstituted Complex Homo sapiens
11 RHOA 387
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 ARF6 382
Invivo Homo sapiens
13 BIN1 274
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
14 PRKCA 5578
Affinity Capture-Western Homo sapiens
15 ARF1 375
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PLD1 is involved
PathwayEvidenceSource
Role of phospholipids in phagocytosis TAS Reactome
Synthesis of PA TAS Reactome
Synthesis of PG TAS Reactome





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