Gene description for OSBPL5
Gene name oxysterol binding protein-like 5
Gene symbol OSBPL5
Other names/aliases OBPH1
ORP5
Species Homo sapiens
 Database cross references - OSBPL5
ExoCarta ExoCarta_114879
Vesiclepedia VP_114879
Entrez Gene 114879
HGNC 16392
MIM 606733
UniProt Q9H0X9  
 OSBPL5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for OSBPL5
Molecular Function
    phosphatidylserine binding GO:0001786 IDA
    phospholipid transporter activity GO:0005548 TAS
    oxysterol binding GO:0008142 NAS
    cholesterol binding GO:0015485 IBA
    cholesterol binding GO:0015485 IDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IDA
    phosphatidylserine transfer activity GO:0140343 IDA
Biological Process
    Golgi to plasma membrane transport GO:0006893 NAS
    cholesterol metabolic process GO:0008203 NAS
    phospholipid transport GO:0015914 IBA
    phospholipid transport GO:0015914 IDA
    cholesterol transport GO:0030301 NAS
    phosphatidylserine acyl-chain remodeling GO:0036150 TAS
    intermembrane lipid transfer GO:0120009 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    endoplasmic reticulum-plasma membrane contact site GO:0140268 IDA
 Experiment description of studies that identified OSBPL5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for OSBPL5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 FTL 2512
Affinity Capture-MS Homo sapiens
3 LIMK1 3984
Affinity Capture-MS Homo sapiens
4 VIPR2  
Affinity Capture-MS Homo sapiens
5 Cd2ap 12488
Affinity Capture-MS Mus musculus
6 KIF14 9928
Affinity Capture-MS Homo sapiens
7 CLTCL1 8218
Affinity Capture-MS Homo sapiens
8 KIFC3 3801
Affinity Capture-MS Homo sapiens
9 MAGEA1  
Affinity Capture-MS Homo sapiens
10 NPC1 4864
Affinity Capture-Western Homo sapiens
11 TMEM9B 56674
Affinity Capture-MS Homo sapiens
12 CDC23 8697
Affinity Capture-MS Homo sapiens
13 RIOK3 8780
Affinity Capture-MS Homo sapiens
14 NAGLU 4669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SNAP25 6616
PCA Homo sapiens
16 OR1M1  
Affinity Capture-MS Homo sapiens
17 ZNF281  
Affinity Capture-MS Homo sapiens
18 MKI67  
Affinity Capture-MS Homo sapiens
19 KLC4 89953
Affinity Capture-MS Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 Mus81  
Affinity Capture-MS Mus musculus
22 PDE2A  
Affinity Capture-MS Homo sapiens
23 LRIF1  
Affinity Capture-MS Homo sapiens
24 Samm50  
Affinity Capture-MS Mus musculus
25 PRR11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 INSRR 3645
Affinity Capture-MS Homo sapiens
27 KLHL23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 P2RY1  
Affinity Capture-MS Homo sapiens
29 Sesn2  
Affinity Capture-MS Mus musculus
30 SSSCA1 10534
Affinity Capture-MS Homo sapiens
31 WIPI1 55062
Affinity Capture-MS Homo sapiens
32 LHFPL1  
Affinity Capture-MS Homo sapiens
33 HGS 9146
Reconstituted Complex Homo sapiens
34 VIPR1  
Affinity Capture-MS Homo sapiens
35 PIGH  
Affinity Capture-MS Homo sapiens
36 OSBPL8 114882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 IPO8 10526
Affinity Capture-MS Homo sapiens
38 PLD1 5337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here