Gene description for NAGLU
Gene name N-acetylglucosaminidase, alpha
Gene symbol NAGLU
Other names/aliases MPS-IIIB
MPS3B
NAG
UFHSD
Species Homo sapiens
 Database cross references - NAGLU
ExoCarta ExoCarta_4669
Entrez Gene 4669
HGNC 7632
MIM 609701
UniProt P54802  
 NAGLU identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 21082674    
 Gene ontology annotations for NAGLU
Molecular Function
    alpha-N-acetylglucosaminidase activity GO:0004561 IEA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    retinal rod cell development GO:0046548 IEA
    cerebellar Purkinje cell layer development GO:0021680 IEA
    lysosome organization GO:0007040 IEA
    glycosaminoglycan catabolic process GO:0006027 TAS
    nervous system development GO:0007399 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    carbohydrate metabolic process GO:0005975 TAS
    inner ear receptor cell development GO:0060119 IEA
    middle ear morphogenesis GO:0042474 IEA
    locomotor rhythm GO:0045475 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    lysosome GO:0005764 TAS
    lysosomal lumen GO:0043202 TAS
 Experiment description of studies that identified NAGLU in exosomes
1
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 102
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS.
Authors Zhang Y, Li Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
 Protein-protein interactions for NAGLU
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NAGLU is involved
PathwayEvidenceSource
HS-GAG degradation TAS Reactome
MPS IIIB - Sanfilippo syndrome B TAS Reactome





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