Gene description for ENPP7
Gene name ectonucleotide pyrophosphatase/phosphodiesterase 7
Gene symbol ENPP7
Other names/aliases ALK-SMase
E-NPP 7
NPP-7
NPP7
Species Homo sapiens
 Database cross references - ENPP7
ExoCarta ExoCarta_339221
Vesiclepedia VP_339221
Entrez Gene 339221
HGNC 23764
UniProt Q6UWV6  
 ENPP7 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for ENPP7
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 IDA
    sphingomyelin phosphodiesterase activity GO:0004767 TAS
    protein binding GO:0005515 IPI
    phosphoric diester hydrolase activity GO:0008081 IDA
    zinc ion binding GO:0008270 IDA
Biological Process
    sphingomyelin metabolic process GO:0006684 IDA
    negative regulation of DNA replication GO:0008156 IDA
    negative regulation of cell population proliferation GO:0008285 IDA
    lipid digestion GO:0044241 ISS
    positive regulation of intestinal cholesterol absorption GO:0045797 ISS
    glycosphingolipid catabolic process GO:0046479 TAS
    fatty acid homeostasis GO:0055089 ISS
    regulation of intestinal lipid absorption GO:1904729 ISS
    positive regulation of ceramide biosynthetic process GO:2000304 ISS
    positive regulation of sphingomyelin catabolic process GO:2000755 ISS
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 IDA
    membrane GO:0016020 NAS
 Experiment description of studies that identified ENPP7 in exosomes
1
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 274
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for ENPP7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOMO1 23420
Affinity Capture-MS Homo sapiens
2 IL17RA 23765
Affinity Capture-MS Homo sapiens
3 EPDR1 54749
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NAAA  
Affinity Capture-MS Homo sapiens
5 TTC17 55761
Affinity Capture-MS Homo sapiens
6 AGPAT1 10554
Affinity Capture-MS Homo sapiens
7 MKS1 54903
Affinity Capture-MS Homo sapiens
8 PDIA4 9601
Affinity Capture-MS Homo sapiens
9 KDELC1 79070
Affinity Capture-MS Homo sapiens
10 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
11 TUBB8 347688
Affinity Capture-MS Homo sapiens
12 ERGIC3 51614
Affinity Capture-MS Homo sapiens
13 TUBB3 10381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 SLC25A15  
Affinity Capture-MS Homo sapiens
15 LRRC73  
Two-hybrid Homo sapiens
16 KRTAP5-9  
Two-hybrid Homo sapiens
17 TUBB1 81027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NAGLU 4669
Affinity Capture-MS Homo sapiens
19 GOLGA2 2801
Two-hybrid Homo sapiens
20 ERGIC2 51290
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RHEB 6009
Affinity Capture-MS Homo sapiens
22 KDELC2 143888
Affinity Capture-MS Homo sapiens
23 NAGA 4668
Affinity Capture-MS Homo sapiens
24 CD109 135228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MTUS2 23281
Two-hybrid Homo sapiens
26 BACH2 60468
Two-hybrid Homo sapiens
27 PDF  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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