Gene description for NAGA
Gene name N-acetylgalactosaminidase, alpha-
Gene symbol NAGA
Other names/aliases D22S674
GALB
Species Homo sapiens
 Database cross references - NAGA
ExoCarta ExoCarta_4668
Entrez Gene 4668
HGNC 7631
MIM 104170
UniProt P17050  
 NAGA identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NAGA
Molecular Function
    alpha-galactosidase activity GO:0004557 IBA
    alpha-N-acetylgalactosaminidase activity GO:0008456 IDA
    protein homodimerization activity GO:0042803 IPI
Biological Process
    glycoside catabolic process GO:0016139 IBA
    glycolipid catabolic process GO:0019377 IMP
    glycosylceramide catabolic process GO:0046477 IBA
    oligosaccharide metabolic process GO:0009311 IBA
    carbohydrate catabolic process GO:0016052 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    lysosome GO:0005764 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified NAGA in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NAGA
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NAGA is involved
No pathways found





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