Gene description for CTSG
Gene name cathepsin G
Gene symbol CTSG
Other names/aliases CATG
CG
Species Homo sapiens
 Database cross references - CTSG
ExoCarta ExoCarta_1511
Vesiclepedia VP_1511
Entrez Gene 1511
HGNC 2532
MIM 116830
UniProt P08311  
 CTSG identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CTSG
Molecular Function
    serine-type endopeptidase activity GO:0004252 EXP
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 TAS
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    peptidase activity GO:0008233 IDA
    peptidase activity GO:0008233 TAS
    serine-type peptidase activity GO:0008236 IMP
    receptor ligand activity GO:0048018 IDA
    caspase binding GO:0089720 IPI
Biological Process
    angiotensin maturation GO:0002003 TAS
    monocyte chemotaxis GO:0002548 IDA
    protein phosphorylation GO:0006468 IDA
    proteolysis GO:0006508 IDA
    proteolysis GO:0006508 IDA
    immune response GO:0006955 TAS
    protein processing GO:0016485 IDA
    cytokine-mediated signaling pathway GO:0019221 TAS
    antibacterial humoral response GO:0019731 IDA
    extracellular matrix disassembly GO:0022617 TAS
    platelet activation GO:0030168 IDA
    purinergic nucleotide receptor signaling pathway GO:0035590 TAS
    neutrophil activation GO:0042119 IDA
    positive regulation of immune response GO:0050778 IEA
    defense response to Gram-negative bacterium GO:0050829 IDA
    defense response to Gram-positive bacterium GO:0050830 IDA
    defense response to fungus GO:0050832 IEA
    negative regulation of T cell activation GO:0050868 IDA
    protein maturation GO:0051604 IBA
    neutrophil-mediated killing of gram-positive bacterium GO:0070946 IEA
    cellular response to lipopolysaccharide GO:0071222 IDA
    biofilm matrix disassembly GO:0098786 IDA
    positive regulation of platelet aggregation GO:1901731 IDA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IDA
    lysosome GO:0005764 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cytoplasmic stress granule GO:0010494 IDA
    membrane GO:0016020 IDA
    secretory granule GO:0030141 IDA
    azurophil granule lumen GO:0035578 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CTSG in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
6
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTSG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HFE  
Affinity Capture-MS Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
3 WIZ 58525
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RPS27A 6233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PPP6R3 55291
Affinity Capture-MS Homo sapiens
6 RHBDD1 84236
Affinity Capture-MS Homo sapiens
7 LRRC17 10234
Affinity Capture-MS Homo sapiens
8 SERPINB4 6318
Reconstituted Complex Homo sapiens
9 PARP1 142
Biochemical Activity Homo sapiens
10 GSK3B 2932
Affinity Capture-MS Homo sapiens
11 AGPAT1 10554
Affinity Capture-MS Homo sapiens
12 SDC1 6382
Reconstituted Complex Homo sapiens
13 YTHDF1 54915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ADAMTS7  
Affinity Capture-MS Homo sapiens
15 OCRL 4952
Affinity Capture-MS Homo sapiens
16 CACNA2D2 9254
Affinity Capture-MS Homo sapiens
17 MRC2 9902
Affinity Capture-MS Homo sapiens
18 GNG8  
Affinity Capture-MS Homo sapiens
19 TUBB8 347688
Affinity Capture-MS Homo sapiens
20 CELSR1 9620
Affinity Capture-MS Homo sapiens
21 DNAJA3 9093
Affinity Capture-MS Homo sapiens
22 FREM2 341640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 KBTBD6  
Affinity Capture-MS Homo sapiens
24 SERPINA3 12
Affinity Capture-MS Homo sapiens
25 KBTBD7  
Affinity Capture-MS Homo sapiens
26 GPRASP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TUBA1A 7846
Affinity Capture-MS Homo sapiens
28 COL6A2 1292
Affinity Capture-MS Homo sapiens
29 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
30 FGFR1 2260
Affinity Capture-MS Homo sapiens
31 MANBA 4126
Affinity Capture-MS Homo sapiens
32 ADAM21  
Affinity Capture-MS Homo sapiens
33 VWDE  
Affinity Capture-MS Homo sapiens
34 FUT11 170384
Affinity Capture-MS Homo sapiens
35 PSMG3 84262
Affinity Capture-MS Homo sapiens
36 CHRNA5  
Affinity Capture-MS Homo sapiens
37 KLK10 5655
Affinity Capture-MS Homo sapiens
38 LRP6 4040
Affinity Capture-MS Homo sapiens
39 TUBB 203068
Affinity Capture-MS Homo sapiens
40 HSPA5 3309
Affinity Capture-MS Homo sapiens
41 CLSTN3 9746
Affinity Capture-MS Homo sapiens
42 NAGA 4668
Affinity Capture-MS Homo sapiens
43 FRAS1 80144
Affinity Capture-MS Homo sapiens
44 EGFL8  
Affinity Capture-MS Homo sapiens
45 LDLR 3949
Affinity Capture-MS Homo sapiens
46 HBB 3043
Affinity Capture-MS Homo sapiens
47 PRKX 5613
Affinity Capture-MS Homo sapiens
48 ICE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 SMIM8  
Affinity Capture-MS Homo sapiens
50 PPP6R1 22870
Affinity Capture-MS Homo sapiens
51 HS1BP3 64342
Affinity Capture-MS Homo sapiens
52 TCF25 22980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CCR1  
Affinity Capture-MS Homo sapiens
54 SDF2L1 23753
Affinity Capture-MS Homo sapiens
55 KRT31 3881
Two-hybrid Homo sapiens
56 CYP3A43  
Affinity Capture-MS Homo sapiens
57 SORL1 6653
Affinity Capture-MS Homo sapiens
58 FAM192A  
Affinity Capture-MS Homo sapiens
59 PASK  
Affinity Capture-MS Homo sapiens
60 RAD21 5885
Affinity Capture-Western Homo sapiens
61 MAEA 10296
Affinity Capture-MS Homo sapiens
62 TCEB3B  
Affinity Capture-MS Homo sapiens
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