Gene description for CTSG
Gene name cathepsin G
Gene symbol CTSG
Other names/aliases CATG
CG
Species Homo sapiens
 Database cross references - CTSG
ExoCarta ExoCarta_1511
Vesiclepedia VP_1511
Entrez Gene 1511
HGNC 2532
MIM 116830
UniProt P08311  
 CTSG identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CTSG
Molecular Function
    peptidase activity GO:0008233 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    serine-type endopeptidase activity GO:0004252 IDA
Biological Process
    neutrophil mediated killing of gram-positive bacterium GO:0070946 IEA
    protein phosphorylation GO:0006468 IDA
    angiotensin maturation GO:0002003 TAS
    extracellular matrix organization GO:0030198 TAS
    immune response GO:0006955 TAS
    extracellular matrix disassembly GO:0022617 TAS
    negative regulation of growth of symbiont in host GO:0044130 IEA
    positive regulation of immune response GO:0050778 IEA
    proteolysis GO:0006508 IDA
    protein processing GO:0016485 IBA
    defense response to fungus GO:0050832 IEA
    response to lipopolysaccharide GO:0032496 IEA
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    extracellular space GO:0005615 IDA
    extracellular matrix GO:0031012 IDA
    cell surface GO:0009986 IEA
    secretory granule GO:0030141 IDA
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified CTSG in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
6
Experiment ID 196
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 197
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CTSG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THBS1 7057
Invitro Homo sapiens
2 GP1BA 2811
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 CAMP 820
Invivo Homo sapiens
Invitro Homo sapiens
4 SERPINB13 5275
Invitro Homo sapiens
5 F2RL2  
Invivo Homo sapiens
6 SERPINA3 12
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 PPBP 5473
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
8 SDC1 6382
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
9 KNG1 3827
Invitro Homo sapiens
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