Gene description for AGPAT1
Gene name 1-acylglycerol-3-phosphate O-acyltransferase 1
Gene symbol AGPAT1
Other names/aliases 1-AGPAT1
G15
LPAAT-alpha
LPAATA
Species Homo sapiens
 Database cross references - AGPAT1
ExoCarta ExoCarta_10554
Vesiclepedia VP_10554
Entrez Gene 10554
HGNC 324
MIM 603099
UniProt Q99943  
 AGPAT1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocytes 26054723    
 Gene ontology annotations for AGPAT1
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IGI
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cellular metabolic process GO:0031325 TAS
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
    cellular lipid metabolic process GO:0044255 TAS
    glycerophospholipid biosynthetic process GO:0046474 TAS
    phosphatidic acid biosynthetic process GO:0006654 TAS
    phospholipid metabolic process GO:0006644 TAS
    positive regulation of cytokine production GO:0001819 IMP
    energy reserve metabolic process GO:0006112 TAS
    triglyceride biosynthetic process GO:0019432 TAS
    positive regulation of cytokine-mediated signaling pathway GO:0001961 IC
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum GO:0005783 TAS
    membrane GO:0016020 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified AGPAT1 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AGPAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AGPAT1 is involved
PathwayEvidenceSource
ChREBP activates metabolic gene expression TAS Reactome
Synthesis of PA TAS Reactome
Triglyceride Biosynthesis TAS Reactome





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