ExoCarta: Gene summary

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Gene description for AGPAT1
Gene name	1-acylglycerol-3-phosphate O-acyltransferase 1
Gene symbol	AGPAT1
Other names/aliases	1-AGPAT1 G15 LPAAT-alpha LPAATA
Species	Homo sapiens
Database cross references - AGPAT1
ExoCarta	ExoCarta_10554
Vesiclepedia	VP_10554
Entrez Gene	10554
HGNC	324
MIM	603099
UniProt	Q99943
AGPAT1 identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Hepatocytes	26054723

Gene ontology annotations for AGPAT1

Molecular Function
	1-acylglycerol-3-phosphate O-acyltransferase activity	GO:0003841	IBA
	1-acylglycerol-3-phosphate O-acyltransferase activity	GO:0003841	IDA
	1-acylglycerol-3-phosphate O-acyltransferase activity	GO:0003841	IGI
	1-acylglycerol-3-phosphate O-acyltransferase activity	GO:0003841	TAS
	protein binding	GO:0005515	IPI
Biological Process
	positive regulation of cytokine production	GO:0001819	IMP
	positive regulation of cytokine-mediated signaling pathway	GO:0001961	IC
	phospholipid metabolic process	GO:0006644	TAS
	phosphatidic acid biosynthetic process	GO:0006654	IBA
	phosphatidic acid biosynthetic process	GO:0006654	IGI
	phosphatidic acid biosynthetic process	GO:0006654	TAS
	CDP-diacylglycerol biosynthetic process	GO:0016024	IEA
Subcellular Localization
	endoplasmic reticulum	GO:0005783	IBA
	endoplasmic reticulum	GO:0005783	IDA
	endoplasmic reticulum membrane	GO:0005789	TAS
	membrane	GO:0016020	HDA

Experiment description of studies that identified AGPAT1 in exosomes

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

237

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	Enriched markers
✔ HSP90B1	Negative markers
✔ qNano	Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocytes

Sample name

MIHA

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RNA Sequencing

Protein-protein interactions for AGPAT1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

CD47

961

Affinity Capture-MS

Homo sapiens

HMOX1

3162

Affinity Capture-MS

Homo sapiens

RAMP3

Affinity Capture-MS

Homo sapiens

GJD3

Proximity Label-MS

Homo sapiens

C5AR2

Affinity Capture-MS

Homo sapiens

KLK15

Affinity Capture-MS

Homo sapiens

CLIP1

6249

Affinity Capture-MS

Homo sapiens

AKR1B10

57016

Affinity Capture-MS

Homo sapiens

COIL

Proximity Label-MS

Homo sapiens

METTL7A

25840

Proximity Label-MS

Homo sapiens

CFHR1

Affinity Capture-MS

Homo sapiens

NAPA

8775

Affinity Capture-MS

Homo sapiens

MNDA

4332

Affinity Capture-MS

Homo sapiens

IL36G

56300

Affinity Capture-MS

Homo sapiens

GSTM5

2949

Affinity Capture-MS

Homo sapiens

2638

Affinity Capture-MS

Homo sapiens

HK3

Affinity Capture-MS

Homo sapiens

ANXA8

653145

Affinity Capture-MS

Homo sapiens

IGHM

3507

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATP12A

479

Affinity Capture-MS

Homo sapiens

MFAP4

4239

Affinity Capture-MS

Homo sapiens

LGALS7B

653499

Affinity Capture-MS

Homo sapiens

HSD17B11

51170

Proximity Label-MS

Homo sapiens

ITIH2

3698

Affinity Capture-MS

Homo sapiens

IL36RN

Affinity Capture-MS

Homo sapiens

IGLC2

3538

Affinity Capture-MS

Homo sapiens

IL36A

Affinity Capture-MS

Homo sapiens

PLG

5340

Affinity Capture-MS

Homo sapiens

ALOX12B

Affinity Capture-MS

Homo sapiens

SBDS

51119

Affinity Capture-MS

Homo sapiens

EVPL

2125

Affinity Capture-MS

Homo sapiens

HAL

3034

Affinity Capture-MS

Homo sapiens

HPX

3263

Affinity Capture-MS

Homo sapiens

APEX1

328

Affinity Capture-RNA

Homo sapiens

KIAA1429

25962

Affinity Capture-MS

Homo sapiens

WTAP

9589

Affinity Capture-MS

Homo sapiens

PPL

5493

Affinity Capture-MS

Homo sapiens

CLIC3

9022

Affinity Capture-MS

Homo sapiens

3240

Affinity Capture-MS

Homo sapiens

B3GAT1

Proximity Label-MS

Homo sapiens

GSDMA

284110

Affinity Capture-MS

Homo sapiens

SBSN

374897

Affinity Capture-MS

Homo sapiens

ORM1

5004

Affinity Capture-MS

Homo sapiens

718

Affinity Capture-MS

Homo sapiens

NAGA

4668

Affinity Capture-MS

Homo sapiens

NDRG2

57447

Affinity Capture-MS

Homo sapiens

KLK10

5655

Affinity Capture-MS

Homo sapiens

STAT3

6774

Affinity Capture-MS

Homo sapiens

S100A9

6280

Affinity Capture-MS

Homo sapiens

KLRC1

Affinity Capture-MS

Homo sapiens

SSR1

6745

Proximity Label-MS

Homo sapiens

OBSL1

23363

Affinity Capture-MS

Homo sapiens

NT5C3A

51251

Affinity Capture-MS

Homo sapiens

HIST2H3PS2

440686

Affinity Capture-MS

Homo sapiens

RHOA

387

Affinity Capture-MS

Homo sapiens

ITIH4

3700

Affinity Capture-MS

Homo sapiens

C3orf52

Affinity Capture-MS

Homo sapiens

DSG3

1830

Affinity Capture-MS

Homo sapiens

IGHA2

3494

Affinity Capture-MS

Homo sapiens

SFN

2810

Affinity Capture-MS

Homo sapiens

NAGK

55577

Affinity Capture-MS

Homo sapiens

SERPINA1

5265

Affinity Capture-MS

Homo sapiens

PRTN3

5657

Affinity Capture-MS

Homo sapiens

TFCP2

7024

Affinity Capture-MS

Homo sapiens

BTNL2

Affinity Capture-MS

Homo sapiens

APOH

350

Affinity Capture-MS

Homo sapiens

PAPL

Affinity Capture-MS

Homo sapiens

ITGAM

3684

Affinity Capture-MS

Homo sapiens

TMEM87A

25963

Affinity Capture-MS

Homo sapiens

MARCKS

4082

Proximity Label-MS

Homo sapiens

ARHGDIB

397

Affinity Capture-MS

Homo sapiens

SERPINF2

5345

Affinity Capture-MS

Homo sapiens

CBR1

873

Affinity Capture-MS

Homo sapiens

VTN

7448

Affinity Capture-MS

Homo sapiens

SERPING1

710

Affinity Capture-MS

Homo sapiens

CHMP4C

92421

Affinity Capture-MS

Homo sapiens

EFNA2

Affinity Capture-MS

Homo sapiens

SCEL

8796

Affinity Capture-MS

Homo sapiens

RDH12

145226

Affinity Capture-MS

Homo sapiens

DPEP2

Affinity Capture-MS

Homo sapiens

LCP1

3936

Affinity Capture-MS

Homo sapiens

POF1B

79983

Affinity Capture-MS

Homo sapiens

KLK6

5653

Affinity Capture-MS

Homo sapiens

HRG

3273

Affinity Capture-MS

Homo sapiens

DBNL

28988

Affinity Capture-MS

Homo sapiens

EMD

2010

Proximity Label-MS

Homo sapiens

C1QC

714

Affinity Capture-MS

Homo sapiens

RAB14

51552

Affinity Capture-MS

Homo sapiens

CALML3

810

Affinity Capture-MS

Homo sapiens

FGA

2243

Affinity Capture-MS

Homo sapiens

STOM

2040

Affinity Capture-MS

Homo sapiens

HIST1H2AG

8969

Affinity Capture-MS

Homo sapiens

A2M

Affinity Capture-MS

Homo sapiens

727

Affinity Capture-MS

Homo sapiens

LRG1

116844

Affinity Capture-MS

Homo sapiens

TNFRSF17

Affinity Capture-MS

Homo sapiens

PLD3

23646

Affinity Capture-MS

Homo sapiens

IDS

Affinity Capture-MS

Homo sapiens

CYB5A

1528

Affinity Capture-MS

Homo sapiens

100

YAP1

10413

Affinity Capture-MS

Homo sapiens

101

FIBIN

Affinity Capture-MS

Homo sapiens

102

IGHG1

3500

Affinity Capture-MS

Homo sapiens

103

APOA2

336

Affinity Capture-MS

Homo sapiens

104

GGH

8836

Affinity Capture-MS

Homo sapiens

105

C1orf54

Affinity Capture-MS

Homo sapiens

106

IFI16

3428

Affinity Capture-MS

Homo sapiens

107

CRP

Affinity Capture-MS

Homo sapiens

108

TTR

7276

Affinity Capture-MS

Homo sapiens

109

MEOX2

Two-hybrid

Homo sapiens

110

CD160

Affinity Capture-MS

Homo sapiens

111

ALDH3A1

218

Affinity Capture-MS

Homo sapiens

112

RPN1

6184

Proximity Label-MS

Homo sapiens

113

TGM1

7051

Affinity Capture-MS

Homo sapiens

114

STEAP4

79689

Affinity Capture-MS

Homo sapiens

115

SERPINB7

8710

Affinity Capture-MS

Homo sapiens

116

FGG

2266

Affinity Capture-MS

Homo sapiens

117

SERPINA3

Affinity Capture-MS

Homo sapiens

118

ELOVL5

60481

Proximity Label-MS

Homo sapiens

119

SERPINB13

5275

Affinity Capture-MS

Homo sapiens

120

SEC61B

10952

Proximity Label-MS

Homo sapiens

121

ENDOU

Affinity Capture-MS

Homo sapiens

122

EHD1

10938

Affinity Capture-MS

Homo sapiens

123

LY86

Affinity Capture-MS

Homo sapiens

124

BAG3

9531

Affinity Capture-Luminescence

Homo sapiens

125

LY6H

Affinity Capture-MS

Homo sapiens

126

735

Affinity Capture-MS

Homo sapiens

127

MPO

4353

Affinity Capture-MS

Homo sapiens

128

S100P

6286

Affinity Capture-MS

Homo sapiens

129

FAM129B

64855

Affinity Capture-MS

Homo sapiens

130

IVL

3713

Affinity Capture-MS

Homo sapiens

131

RPN2

6185

Proximity Label-MS

Homo sapiens

132

TYMP

1890

Affinity Capture-MS

Homo sapiens

133

IGHG4

3503

Affinity Capture-MS

Homo sapiens

134

HEPHL1

Affinity Capture-MS

Homo sapiens

135

IGHA1

3493

Affinity Capture-MS

Homo sapiens

136

IL1RN

3557

Affinity Capture-MS

Homo sapiens

137

RAP1B

5908

Affinity Capture-MS

Homo sapiens

138

CRYAB

1410

Affinity Capture-MS

Homo sapiens

139

C4BPA

722

Affinity Capture-MS

Homo sapiens

140

IGKC

3514

Affinity Capture-MS

Homo sapiens

141

SERPINA9

327657

Affinity Capture-MS

Homo sapiens

142

BCAP31

10134

Proximity Label-MS

Homo sapiens

143

HSD3B7

80270

Proximity Label-MS

Homo sapiens

144

UGP2

7360

Affinity Capture-MS

Homo sapiens

145

S100A8

6279

Affinity Capture-MS

Homo sapiens

146

1356

Affinity Capture-MS

Homo sapiens

147

KLRD1

Affinity Capture-MS

Homo sapiens

148

ARF6

382

Proximity Label-MS

Homo sapiens

149

S100A7A

338324

Affinity Capture-MS

Homo sapiens

150

CFI

3426

Affinity Capture-MS

Homo sapiens

151

FN1

2335

Affinity Capture-MS

Homo sapiens

152

APCS

325

Affinity Capture-MS

Homo sapiens

153

NXF1

10482

Affinity Capture-RNA

Homo sapiens

154

Hoxa1

Two-hybrid

Mus musculus

155

CTSG

1511

Affinity Capture-MS

Homo sapiens

156

HIST1H1B

3009

Affinity Capture-MS

Homo sapiens

157

TJP2

9414

Affinity Capture-MS

Homo sapiens

158

SERPINF1

5176

Affinity Capture-MS

Homo sapiens

159

EPS8L1

54869

Affinity Capture-MS

Homo sapiens

160

RPA3

6119

Proximity Label-MS

Homo sapiens

161

ATP2A1

487

Proximity Label-MS

Homo sapiens

162

RPS4Y1

6192

Affinity Capture-MS

Homo sapiens

163

PSCA

8000

Affinity Capture-MS

Homo sapiens

164

TIGAR

57103

Affinity Capture-MS

Homo sapiens

165

KNG1

3827

Affinity Capture-MS

Homo sapiens

166

C8G

733

Affinity Capture-MS

Homo sapiens

167

ENPP7

339221

Affinity Capture-MS

Homo sapiens

168

ISLR

3671

Affinity Capture-MS

Homo sapiens

169

SDR9C7

Affinity Capture-MS

Homo sapiens

170

P2RX2

Affinity Capture-MS

Homo sapiens

171

H2AFY

9555

Affinity Capture-MS

Homo sapiens

172

UPF2

26019

Two-hybrid

Homo sapiens

173

A2ML1

144568

Affinity Capture-MS

Homo sapiens

174

CFB

629

Affinity Capture-MS

Homo sapiens

175

H2AFX

3014

Affinity Capture-MS

Homo sapiens

176

TMEM40

55287

Affinity Capture-MS

Homo sapiens

177

FERMT1

55612

Affinity Capture-MS

Homo sapiens

178

JMJD7-PLA2G4B

Affinity Capture-MS

Homo sapiens

179

CLPP

8192

Proximity Label-MS

Homo sapiens

180

RNASE7

84659

Affinity Capture-MS

Homo sapiens

181

RAB5B

5869

Affinity Capture-MS

Homo sapiens

182

TRIM29

23650

Affinity Capture-MS

Homo sapiens

183

TCTN1

Affinity Capture-MS

Homo sapiens

184

IGHD

Affinity Capture-MS

Homo sapiens

185

LDLRAD1

Affinity Capture-MS

Homo sapiens

186

CALML5

51806

Affinity Capture-MS

Homo sapiens

187

PYCARD

29108

Affinity Capture-MS

Homo sapiens

188

IGLL5

100423062

Affinity Capture-MS

Homo sapiens

189

ALPPL2

251

Affinity Capture-MS

Homo sapiens

190

RAB38

23682

Affinity Capture-MS

Homo sapiens

191

CFH

3075

Affinity Capture-MS

Homo sapiens

192

HAVCR2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

193

EFNA1

Affinity Capture-MS

Homo sapiens

194

FGB

2244

Affinity Capture-MS

Homo sapiens

195

SERPINB4

6318

Affinity Capture-MS

Homo sapiens

196

OXSR1

9943

Affinity Capture-MS

Homo sapiens

197

ELAVL1

1994

Affinity Capture-RNA

Homo sapiens

198

IGHG2

3501

Affinity Capture-MS

Homo sapiens

199

TREX2

Affinity Capture-MS

Homo sapiens

200

S100A7

6278

Affinity Capture-MS

Homo sapiens

201

ERGIC1

57222

Proximity Label-MS

Homo sapiens

202

C11orf52

91894

Proximity Label-MS

Homo sapiens

203

GPX3

2878

Affinity Capture-MS

Homo sapiens

204

SERPINB3

6317

Affinity Capture-MS

Homo sapiens

205

PRSS27

Affinity Capture-MS

Homo sapiens

206

7018

Affinity Capture-MS

Homo sapiens

207

SFTPC

Affinity Capture-MS

Homo sapiens

208

RNASEH2A

10535

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

209

IDH2

3418

Affinity Capture-MS

Homo sapiens

210

TMEM25

Affinity Capture-MS

Homo sapiens

211

ITIH1

3697

Affinity Capture-MS

Homo sapiens

212

MOV10

4343

Affinity Capture-RNA

Homo sapiens

213

TGM3

7053

Affinity Capture-MS

Homo sapiens

214

SEC62

7095

Proximity Label-MS

Homo sapiens

215

GM2A

2760

Affinity Capture-MS

Homo sapiens

216

2147

Affinity Capture-MS

Homo sapiens

217

APOBEC3A

200315

Affinity Capture-MS

Homo sapiens

218

ZNF185

7739

Affinity Capture-MS

Homo sapiens

219

SERPIND1

3053

Affinity Capture-MS

Homo sapiens

220

PLA2G4D

Affinity Capture-MS

Homo sapiens

221

SERPINB2

5055

Affinity Capture-MS

Homo sapiens

222

KYNU

Affinity Capture-MS

Homo sapiens

223

NCCRP1

342897

Affinity Capture-MS

Homo sapiens

224

IGHG3

3502

Affinity Capture-MS

Homo sapiens

225

CST11

Affinity Capture-MS

Homo sapiens

226

CYB5R2

51700

Affinity Capture-MS

Homo sapiens

227

RAB5C

5878

Proximity Label-MS

Homo sapiens

228

ALB

213

Affinity Capture-MS

Homo sapiens

229

LCN2

3934

Affinity Capture-MS

Homo sapiens

230

LTF

4057

Affinity Capture-MS

Homo sapiens

231

CKAP4

10970

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

232

VIM

7431

Affinity Capture-MS

Homo sapiens

233

PTCH1

Affinity Capture-MS

Homo sapiens

234

AHNAK2

113146

Affinity Capture-MS

Homo sapiens

235

CLEC4E

Affinity Capture-MS

Homo sapiens

236

S100A2

6273

Affinity Capture-MS

Homo sapiens

237

SULT2B1

6820

Affinity Capture-MS

Homo sapiens

238

AIM1

202

Affinity Capture-MS

Homo sapiens

239

APOA1

335

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which AGPAT1 is involved

Pathway	Evidence	Source
ChREBP activates metabolic gene expression	TAS	Reactome
Glycerophospholipid biosynthesis	TAS	Reactome
Integration of energy metabolism	TAS	Reactome
Metabolism	TAS	Reactome
Metabolism of lipids	TAS	Reactome
Phospholipid metabolism	TAS	Reactome
Synthesis of PA	TAS	Reactome

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