Gene description for DSG3
Gene name desmoglein 3
Gene symbol DSG3
Other names/aliases CDHF6
PVA
Species Homo sapiens
 Database cross references - DSG3
ExoCarta ExoCarta_1830
Vesiclepedia VP_1830
Entrez Gene 1830
HGNC 3050
MIM 169615
UniProt P32926  
 DSG3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for DSG3
Molecular Function
    calcium ion binding GO:0005509 IEA
Biological Process
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    programmed cell death GO:0012501 TAS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    apoptotic process GO:0006915 TAS
Subcellular Localization
    desmosome GO:0030057 IEA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified DSG3 in exosomes
1
Experiment ID 282
MISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 275
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
4
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DSG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PKP3 11187
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 JUP 3728
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DSG3 is involved
PathwayEvidenceSource
Apoptotic cleavage of cell adhesion proteins TAS Reactome





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