Gene ontology annotations for JUP
Experiment description of studies that identified JUP in sEVs
1
Experiment ID
76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
2
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
6
Experiment ID
412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
7
Experiment ID
414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
8
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Osteoarthritic cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
496
MISEV standards
✘
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Healthy cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
12
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
13
Experiment ID
498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
14
Experiment ID
20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
15
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
17
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
18
Experiment ID
282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
19
Experiment ID
283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
20
Experiment ID
285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
21
Experiment ID
286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
22
Experiment ID
1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
23
Experiment ID
407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
24
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
26
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
27
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
28
Experiment ID
405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
29
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
30
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
31
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-C3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
33
Experiment ID
236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
34
Experiment ID
266
MISEV standards
✘
Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Wild type
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
35
Experiment ID
267
MISEV standards
✘
Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
36
Experiment ID
268
MISEV standards
✘
Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
37
Experiment ID
417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
38
Experiment ID
411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
39
Experiment ID
258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
40
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
41
Experiment ID
488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
42
Experiment ID
418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
43
Experiment ID
418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
44
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
45
Experiment ID
413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
46
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
47
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
48
Experiment ID
406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
49
Experiment ID
415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
50
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
51
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
52
Experiment ID
408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
53
Experiment ID
409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
54
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
55
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
56
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
57
Experiment ID
416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
58
Experiment ID
275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
59
Experiment ID
274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
60
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
61
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
62
Experiment ID
66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
63
Experiment ID
410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
64
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
65
Experiment ID
63
MISEV standards
✘
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for JUP
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
GJD3
Proximity Label-MS
Homo sapiens
2
PRR35
Two-hybrid
Homo sapiens
3
HDAC2
3066
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
4
ATG9A
79065
Proximity Label-MS
Homo sapiens
5
NRSN1
Affinity Capture-MS
Homo sapiens
6
PKP2
5318
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
7
CDH5
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
8
BRCA1
672
Two-hybrid
Homo sapiens
9
KIF20A
10112
Affinity Capture-MS
Homo sapiens
10
CSTA
1475
Affinity Capture-MS
Homo sapiens
11
VHL
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
12
ST7
Affinity Capture-MS
Homo sapiens
13
PRKDC
5591
Co-fractionation
Homo sapiens
14
Nhsl1
Affinity Capture-MS
Mus musculus
15
APEX1
328
Affinity Capture-MS
Homo sapiens
16
PTRH2
51651
Co-fractionation
Homo sapiens
17
IARS2
55699
Co-fractionation
Homo sapiens
18
CDH2
1000
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
19
NUDT3
11165
Affinity Capture-MS
Homo sapiens
20
HSPH1
10808
Affinity Capture-MS
Homo sapiens
21
DYNLL1
8655
Affinity Capture-MS
Homo sapiens
22
KPRP
448834
Affinity Capture-MS
Homo sapiens
23
BRF2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
24
BTRC
8945
Affinity Capture-Western
Homo sapiens
25
ZFYVE9
Two-hybrid
Homo sapiens
26
GMCL1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
27
MYL6
4637
Affinity Capture-MS
Homo sapiens
28
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
29
PIP4K2C
79837
Co-fractionation
Homo sapiens
30
RAD51AP1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
31
ARG1
383
Affinity Capture-MS
Homo sapiens
32
CDH1
999
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Proximity Label-MS
Homo sapiens
Co-fractionation
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
33
YAP1
10413
Affinity Capture-MS
Homo sapiens
34
MYH9
4627
Affinity Capture-MS
Homo sapiens
35
TRIM21
6737
Affinity Capture-MS
Homo sapiens
36
SLX4
Affinity Capture-MS
Homo sapiens
37
PIGT
51604
Affinity Capture-MS
Homo sapiens
38
DTX2
113878
Proximity Label-MS
Homo sapiens
39
OR2A4
79541
Affinity Capture-MS
Homo sapiens
40
GJB1
2705
Affinity Capture-Western
Homo sapiens
41
Coro1c
23790
Affinity Capture-MS
Mus musculus
42
CUL2
8453
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
43
DDX39B
7919
Affinity Capture-MS
Homo sapiens
44
VDAC2
7417
Co-fractionation
Homo sapiens
45
VCP
7415
Affinity Capture-MS
Homo sapiens
46
YWHAE
7531
Affinity Capture-MS
Homo sapiens
47
ANLN
54443
Affinity Capture-MS
Homo sapiens
48
PHLPP1
Proximity Label-MS
Homo sapiens
49
BIRC2
Two-hybrid
Homo sapiens
50
CTNNA2
1496
Co-fractionation
Homo sapiens
51
HSPA5
3309
Affinity Capture-MS
Homo sapiens
52
PPP2R2B
5521
Affinity Capture-MS
Homo sapiens
53
GMPPB
29925
Co-fractionation
Homo sapiens
54
LMNB1
4001
Co-fractionation
Homo sapiens
55
EPAS1
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-localization
Homo sapiens
56
GOLT1B
51026
Affinity Capture-MS
Homo sapiens
57
ATP6AP2
10159
Affinity Capture-MS
Homo sapiens
58
DES
1674
Co-fractionation
Homo sapiens
59
TBP
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
60
SOAT1
6646
Affinity Capture-MS
Homo sapiens
61
RPA3
6119
Proximity Label-MS
Homo sapiens
62
NME1
4830
Affinity Capture-MS
Homo sapiens
63
HSPA4
3308
Co-fractionation
Homo sapiens
64
PSMC5
5705
Affinity Capture-MS
Homo sapiens
65
CTNNA1
1495
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Proximity Label-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-localization
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
66
CSDE1
7812
Co-fractionation
Homo sapiens
67
DPYSL3
1809
Co-fractionation
Homo sapiens
68
METTL14
Affinity Capture-MS
Homo sapiens
69
GRK5
2869
Affinity Capture-MS
Homo sapiens
70
FASN
2194
Affinity Capture-MS
Homo sapiens
71
PLEC
5339
Co-fractionation
Homo sapiens
72
PSAT1
29968
Affinity Capture-MS
Homo sapiens
73
USP1
7398
Affinity Capture-MS
Homo sapiens
74
WWOX
51741
Affinity Capture-MS
Homo sapiens
75
LCK
3932
Proximity Label-MS
Homo sapiens
76
APRT
353
Affinity Capture-MS
Homo sapiens
77
RUVBL2
10856
Affinity Capture-MS
Homo sapiens
78
PTDSS1
9791
Affinity Capture-MS
Homo sapiens
79
HSP90AA1
3320
Affinity Capture-MS
Homo sapiens
80
TARS
6897
Affinity Capture-MS
Homo sapiens
81
ERCC3
Affinity Capture-MS
Homo sapiens
82
DDX3X
1654
Affinity Capture-MS
Homo sapiens
83
TCF4
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
84
GAPDH
2597
Affinity Capture-MS
Homo sapiens
85
Cobll1
319876
Affinity Capture-MS
Mus musculus
86
ZSCAN12
Affinity Capture-MS
Homo sapiens
87
RAB11A
8766
Proximity Label-MS
Homo sapiens
88
EPHA2
1969
Proximity Label-MS
Homo sapiens
89
PDE4DIP
9659
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
90
CUL7
9820
Affinity Capture-MS
Homo sapiens
91
ANXA2
302
Affinity Capture-MS
Homo sapiens
92
GABPA
2551
Affinity Capture-MS
Homo sapiens
93
CTNND1
1500
Affinity Capture-MS
Homo sapiens
94
PTPRF
5792
Reconstituted Complex
Homo sapiens
95
MCM3
4172
Co-fractionation
Homo sapiens
96
KIF23
9493
Affinity Capture-MS
Homo sapiens
97
KLC2
64837
Co-fractionation
Homo sapiens
98
METTL7A
25840
Affinity Capture-MS
Homo sapiens
99
GJA1
2697
Proximity Label-MS
Homo sapiens
100
TARDBP
23435
Affinity Capture-MS
Homo sapiens
101
RPA2
6118
Proximity Label-MS
Homo sapiens
102
CDK15
Affinity Capture-MS
Homo sapiens
103
APC
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-localization
Homo sapiens
104
DBN1
1627
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
105
SPSB4
Affinity Capture-MS
Homo sapiens
106
HSP90AB1
3326
Affinity Capture-MS
Homo sapiens
107
NENF
29937
Co-fractionation
Homo sapiens
108
RABGAP1L
9910
Co-fractionation
Homo sapiens
109
S100A14
57402
Affinity Capture-MS
Homo sapiens
110
B3GNT2
10678
Affinity Capture-MS
Homo sapiens
111
RBM3
5935
Affinity Capture-MS
Homo sapiens
112
TLN1
7094
Co-fractionation
Homo sapiens
113
Dsc1
Affinity Capture-Western
Mus musculus
114
MUC1
4582
Affinity Capture-Western
Homo sapiens
Co-localization
Homo sapiens
115
NPM1
4869
Co-fractionation
Homo sapiens
116
NARS
4677
Co-fractionation
Homo sapiens
117
PRDX2
7001
Affinity Capture-MS
Homo sapiens
118
Myh9
17886
Affinity Capture-MS
Mus musculus
119
PTPN14
5784
Affinity Capture-MS
Homo sapiens
120
TRMT1
55621
Co-fractionation
Homo sapiens
121
DDX6
1656
Two-hybrid
Homo sapiens
122
ABCC2
1244
Co-fractionation
Homo sapiens
123
CCDC130
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
124
OAZ3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
125
EBAG9
9166
Proximity Label-MS
Homo sapiens
126
DSG1
1828
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
127
LYN
4067
Proximity Label-MS
Homo sapiens
128
RPS6KB1
6198
Co-fractionation
Homo sapiens
129
PMAIP1
Affinity Capture-MS
Homo sapiens
130
CTNNB1
1499
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
Co-localization
Homo sapiens
Co-fractionation
Homo sapiens
131
CTNNA3
Two-hybrid
Homo sapiens
132
UPK1A
11045
Affinity Capture-MS
Homo sapiens
133
MRPL9
65005
Affinity Capture-MS
Homo sapiens
134
CYLD
Affinity Capture-MS
Homo sapiens
135
YEATS4
Affinity Capture-MS
Homo sapiens
136
CAT
847
Affinity Capture-MS
Homo sapiens
137
CASP14
23581
Affinity Capture-MS
Homo sapiens
138
RAB7A
7879
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
139
RPS2
6187
Affinity Capture-MS
Homo sapiens
140
RAB5A
5868
Proximity Label-MS
Homo sapiens
141
SLC35F2
54733
Affinity Capture-MS
Homo sapiens
142
CDH3
1001
Affinity Capture-Western
Homo sapiens
Co-purification
Homo sapiens
143
EGFR
1956
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
PCA
Homo sapiens
Biochemical Activity
Homo sapiens
144
HSD17B10
3028
Affinity Capture-MS
Homo sapiens
145
PTK2
5747
Co-fractionation
Homo sapiens
146
LGALS7
3963
Affinity Capture-MS
Homo sapiens
147
C21orf59
56683
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
148
Flot1
14251
Affinity Capture-MS
Mus musculus
149
CDK2
1017
Affinity Capture-MS
Homo sapiens
150
MYC
Affinity Capture-MS
Homo sapiens
151
RIBC2
Two-hybrid
Homo sapiens
152
RHOB
388
Proximity Label-MS
Homo sapiens
153
NFYA
4800
Co-fractionation
Homo sapiens
154
Ppp1cb
19046
Affinity Capture-MS
Mus musculus
155
FHL2
2274
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
156
STX17
55014
Affinity Capture-MS
Homo sapiens
157
RIPPLY3
Affinity Capture-MS
Homo sapiens
158
PHGDH
26227
Affinity Capture-MS
Homo sapiens
159
OST4
Affinity Capture-MS
Homo sapiens
160
DSP
1832
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
161
EEF1A1
1915
Affinity Capture-MS
Homo sapiens
162
SPOP
Affinity Capture-MS
Homo sapiens
163
KIF14
9928
Affinity Capture-MS
Homo sapiens
164
ILF3
3609
Co-fractionation
Homo sapiens
165
MAX
Co-fractionation
Homo sapiens
166
XRCC6
2547
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
167
SAMHD1
25939
Co-fractionation
Homo sapiens
168
DSC1
1823
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
169
MAP2K1
5604
Affinity Capture-MS
Homo sapiens
170
CDK1
983
Affinity Capture-MS
Homo sapiens
171
KLHL11
Affinity Capture-MS
Homo sapiens
172
ESR1
Affinity Capture-MS
Homo sapiens
173
KLF5
Affinity Capture-MS
Homo sapiens
174
COQ2
Affinity Capture-MS
Homo sapiens
175
BCL2L1
598
Affinity Capture-MS
Homo sapiens
176
CALML5
51806
Affinity Capture-MS
Homo sapiens
177
GOT1
2805
Affinity Capture-MS
Homo sapiens
178
C1QBP
708
Co-fractionation
Homo sapiens
179
HSPA4L
22824
Co-fractionation
Homo sapiens
180
BCAR1
9564
Co-fractionation
Homo sapiens
181
PRMT1
3276
Affinity Capture-MS
Homo sapiens
182
FLG2
388698
Affinity Capture-MS
Homo sapiens
183
ATP6V1B2
526
Co-fractionation
Homo sapiens
184
OCLN
100506658
Proximity Label-MS
Homo sapiens
185
MARCKS
4082
Proximity Label-MS
Homo sapiens
186
PARD3
56288
Proximity Label-MS
Homo sapiens
187
Chmp4b
75608
Affinity Capture-MS
Mus musculus
188
RPS19
6223
Affinity Capture-MS
Homo sapiens
189
TCF7L2
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
190
ARCN1
372
Affinity Capture-MS
Homo sapiens
191
STX4
6810
Proximity Label-MS
Homo sapiens
192
PI4KA
5297
Affinity Capture-MS
Homo sapiens
193
ARHGDIA
396
Two-hybrid
Homo sapiens
194
LRPPRC
10128
Affinity Capture-MS
Homo sapiens
195
CAPZB
832
Affinity Capture-MS
Homo sapiens
196
RPL10A
4736
Affinity Capture-MS
Homo sapiens
197
TRIM28
10155
Co-fractionation
Homo sapiens
198
MLLT4
4301
Proximity Label-MS
Homo sapiens
199
IGF1R
3480
Affinity Capture-MS
Homo sapiens
200
MCM2
4171
Affinity Capture-MS
Homo sapiens
201
LIG1
3978
Co-fractionation
Homo sapiens
202
FLNA
2316
Affinity Capture-MS
Homo sapiens
203
DSC2
1824
Affinity Capture-Western
Homo sapiens
204
KRAS
3845
Affinity Capture-MS
Homo sapiens
Proximity Label-MS
Homo sapiens
205
HMGA2
8091
Co-fractionation
Homo sapiens
206
DSG3
1830
Affinity Capture-Western
Homo sapiens
207
RAB8B
51762
Affinity Capture-Western
Homo sapiens
208
B4GALT1
2683
Co-fractionation
Homo sapiens
209
LGALS9
3965
Affinity Capture-MS
Homo sapiens
210
TFCP2
7024
Affinity Capture-MS
Homo sapiens
211
DSC3
1825
Affinity Capture-Western
Homo sapiens
212
INSIG2
Affinity Capture-MS
Homo sapiens
213
DLD
1738
Affinity Capture-MS
Homo sapiens
214
ST6GALNAC6
30815
Affinity Capture-MS
Homo sapiens
215
CUL4B
8450
Affinity Capture-MS
Homo sapiens
216
PARK2
Affinity Capture-MS
Homo sapiens
217
ZDHHC19
Affinity Capture-MS
Homo sapiens
218
PLOD2
5352
Co-fractionation
Homo sapiens
219
SRRT
51593
Affinity Capture-MS
Homo sapiens
220
DNAJC5
80331
Proximity Label-MS
Homo sapiens
221
CCDC51
79714
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
222
CHMP4B
128866
Affinity Capture-MS
Homo sapiens
223
DIRAS3
Proximity Label-MS
Homo sapiens
224
NTRK1
4914
Affinity Capture-MS
Homo sapiens
225
ARNT
405
Affinity Capture-Western
Homo sapiens
226
AQP12B
Affinity Capture-MS
Homo sapiens
227
SCN3B
Affinity Capture-MS
Homo sapiens
228
PECAM1
5175
Affinity Capture-Western
Homo sapiens
229
NFATC4
4776
Two-hybrid
Homo sapiens
230
Rab5c
19345
Affinity Capture-MS
Mus musculus
231
EFNA4
Affinity Capture-MS
Homo sapiens
232
AHCY
191
Affinity Capture-MS
Homo sapiens
233
CDC73
Affinity Capture-MS
Homo sapiens
234
MSH2
4436
Co-fractionation
Homo sapiens
235
ECT2
1894
Affinity Capture-MS
Homo sapiens
236
LILRA3
Affinity Capture-MS
Homo sapiens
237
EEF2
1938
Affinity Capture-MS
Homo sapiens
238
HRNR
388697
Affinity Capture-MS
Homo sapiens
239
LMNB2
84823
Co-fractionation
Homo sapiens
240
RLIM
51132
Affinity Capture-MS
Homo sapiens
241
RPL21
6144
Co-fractionation
Homo sapiens
242
HNRNPA1
3178
Affinity Capture-MS
Homo sapiens
243
ZIC1
Affinity Capture-MS
Homo sapiens
244
SYNPO
11346
Affinity Capture-MS
Homo sapiens
245
PI4KAP1
Affinity Capture-MS
Homo sapiens
246
Flnb
286940
Affinity Capture-MS
Mus musculus
247
RPS13
6207
Affinity Capture-MS
Homo sapiens
248
TOP1
7150
Co-fractionation
Homo sapiens
249
RPL12
6136
Affinity Capture-MS
Homo sapiens
250
PARP1
142
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
251
MSN
4478
Co-fractionation
Homo sapiens
252
FLOT1
10211
Proximity Label-MS
Homo sapiens
253
XRCC5
7520
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
254
HIF1A
3091
Affinity Capture-Western
Homo sapiens
255
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
256
PHB2
11331
Affinity Capture-MS
Homo sapiens
257
TP53
7157
Affinity Capture-MS
Homo sapiens
258
RBM24
221662
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
259
MCM5
4174
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
260
HSPB1
3315
Affinity Capture-MS
Homo sapiens
261
CREBBP
Affinity Capture-Western
Homo sapiens
262
C11orf52
91894
Proximity Label-MS
Homo sapiens
263
MAPK7
Affinity Capture-MS
Homo sapiens
264
NFYB
Co-fractionation
Homo sapiens
265
SERPINB12
89777
Affinity Capture-MS
Homo sapiens
266
TSPAN6
7105
Affinity Capture-MS
Homo sapiens
267
LIPA
3988
Co-fractionation
Homo sapiens
268
RPL28
6158
Affinity Capture-MS
Homo sapiens
269
SERBP1
26135
Affinity Capture-MS
Homo sapiens
270
AR
367
Affinity Capture-MS
Homo sapiens
271
CCR1
Affinity Capture-MS
Homo sapiens
272
PDE3A
Co-fractionation
Homo sapiens
273
CAV1
857
Proximity Label-MS
Homo sapiens
274
EP300
2033
Affinity Capture-Western
Homo sapiens
275
UBE2H
7328
Affinity Capture-MS
Homo sapiens
276
Tpm1
22003
Affinity Capture-MS
Mus musculus
277
RPS3A
6189
Affinity Capture-MS
Homo sapiens
278
Cdh1
12550
Affinity Capture-MS
Mus musculus
Affinity Capture-Western
Mus musculus
279
CDH8
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
280
NFYC
4802
Co-fractionation
Homo sapiens
281
CALM1
801
Affinity Capture-MS
Homo sapiens
282
ILF2
3608
Co-fractionation
Homo sapiens
283
KIAA1429
25962
Affinity Capture-MS
Homo sapiens
284
GPATCH2L
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
285
PRC1
9055
Affinity Capture-MS
Homo sapiens
286
COPS5
10987
Affinity Capture-MS
Homo sapiens
287
FBXW7
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
288
DDOST
1650
Affinity Capture-MS
Homo sapiens
289
ABCE1
6059
Affinity Capture-MS
Homo sapiens
290
STX6
10228
Proximity Label-MS
Homo sapiens
291
EED
Affinity Capture-MS
Homo sapiens
292
CDH10
Two-hybrid
Homo sapiens
293
RAB35
11021
Proximity Label-MS
Homo sapiens
294
MYCN
Affinity Capture-MS
Homo sapiens
295
Myh10
77579
Affinity Capture-MS
Mus musculus
296
ERBB2
2064
Far Western
Homo sapiens
Co-localization
Homo sapiens
297
OBSL1
23363
Affinity Capture-MS
Homo sapiens
298
LAMTOR1
55004
Proximity Label-MS
Homo sapiens
299
TSHZ2
Affinity Capture-MS
Homo sapiens
300
PPP1CA
5499
Co-fractionation
Homo sapiens
301
SDHA
6389
Affinity Capture-MS
Homo sapiens
302
VCL
7414
Co-fractionation
Homo sapiens
303
WDYHV1
Two-hybrid
Homo sapiens
304
SLC25A44
Co-fractionation
Homo sapiens
305
SSUH2
Affinity Capture-MS
Homo sapiens
306
GANAB
23193
Co-fractionation
Homo sapiens
307
BRAF
Affinity Capture-MS
Homo sapiens
308
CTNNBIP1
56998
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
309
ST13
6767
Co-fractionation
Homo sapiens
310
C1orf68
100129271
Affinity Capture-MS
Homo sapiens
311
LIMA1
51474
Affinity Capture-MS
Homo sapiens
312
NFKBIE
Two-hybrid
Homo sapiens
313
ACTN1
87
Co-fractionation
Homo sapiens
314
RPLP2
6181
Affinity Capture-MS
Homo sapiens
315
FANCD2
Affinity Capture-MS
Homo sapiens
316
VPS4B
9525
Affinity Capture-MS
Homo sapiens
317
TRIM31
Affinity Capture-MS
Homo sapiens
318
POLR2H
5437
Co-fractionation
Homo sapiens
319
EPB41L4A
64097
Proximity Label-MS
Homo sapiens
320
LAMP1
3916
Proximity Label-MS
Homo sapiens
321
Bmpr1a
Affinity Capture-MS
Mus musculus
322
NPPA
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
323
ZC3HC1
Affinity Capture-MS
Homo sapiens
324
ARF6
382
Proximity Label-MS
Homo sapiens
325
CFI
3426
Affinity Capture-MS
Homo sapiens
326
MYO6
4646
Affinity Capture-MS
Homo sapiens
327
FABP5
2171
Affinity Capture-MS
Homo sapiens
328
PTPRK
5796
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
329
CLTC
1213
Affinity Capture-MS
Homo sapiens
330
ITGB4
3691
Co-fractionation
Homo sapiens
331
NSMAF
Affinity Capture-MS
Homo sapiens
332
UBXN1
51035
Affinity Capture-MS
Homo sapiens
333
MGEA5
10724
Co-fractionation
Homo sapiens
334
CCDC8
Affinity Capture-MS
Homo sapiens
335
PKP3
11187
Two-hybrid
Homo sapiens
336
SPTAN1
6709
Affinity Capture-MS
Homo sapiens
337
Tmod3
50875
Affinity Capture-MS
Mus musculus
338
SMURF1
57154
Affinity Capture-MS
Homo sapiens
339
HDAC1
3065
Affinity Capture-Western
Homo sapiens
Co-localization
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
340
AXIN1
Affinity Capture-MS
Homo sapiens
341
Dsg1a
Affinity Capture-Western
Mus musculus
342
CAD
790
Affinity Capture-MS
Homo sapiens
343
TRIP4
9325
Affinity Capture-MS
Homo sapiens
344
PXN
5829
Co-fractionation
Homo sapiens
345
PINK1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
346
ZUFSP
221302
Affinity Capture-MS
Homo sapiens
347
ALDOA
226
Affinity Capture-MS
Homo sapiens
348
DSG2
1829
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
349
Apc
Affinity Capture-MS
Mus musculus
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Pathways in which JUP is involved