Gene description for DSC3
Gene name desmocollin 3
Gene symbol DSC3
Other names/aliases CDHF3
DSC
DSC1
DSC2
DSC4
HT-CP
Species Homo sapiens
 Database cross references - DSC3
ExoCarta ExoCarta_1825
Vesiclepedia VP_1825
Entrez Gene 1825
HGNC 3037
MIM 600271
UniProt Q14574  
 DSC3 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DSC3
Molecular Function
    calcium ion binding GO:0005509 IBA
    gamma-catenin binding GO:0045295 IPI
Biological Process
    in utero embryonic development GO:0001701 IEA
    cell adhesion GO:0007155 TAS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    protein stabilization GO:0050821 IDA
    cell-cell adhesion GO:0098609 IBA
Subcellular Localization
    cornified envelope GO:0001533 TAS
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 TAS
    membrane GO:0016020 TAS
    cell junction GO:0030054 IDA
    desmosome GO:0030057 IBA
 Experiment description of studies that identified DSC3 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DSC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RYK 6259
Affinity Capture-MS Homo sapiens
3 JUP 3728
Affinity Capture-Western Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 CLEC4A  
Affinity Capture-MS Homo sapiens
6 LAMTOR1 55004
Proximity Label-MS Homo sapiens
7 C18orf21  
Affinity Capture-MS Homo sapiens
8 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
9 CANX 821
Affinity Capture-MS Homo sapiens
10 PTPRD 5789
Proximity Label-MS Homo sapiens
11 METTL7A 25840
Proximity Label-MS Homo sapiens
12 ATG9A 79065
Proximity Label-MS Homo sapiens
13 CCR1  
Affinity Capture-MS Homo sapiens
14 FCGRT 2217
Affinity Capture-MS Homo sapiens
15 CTNNB1 1499
Affinity Capture-Western Homo sapiens
16 TMEM30B 161291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSD3B7 80270
Proximity Label-MS Homo sapiens
18 RNF38  
Affinity Capture-MS Homo sapiens
19 NRSN1  
Affinity Capture-MS Homo sapiens
20 TP53 7157
Affinity Capture-MS Homo sapiens
21 ZC3HC1  
Affinity Capture-MS Homo sapiens
22 FBXO6 26270
Affinity Capture-MS Homo sapiens
23 ZDHHC19  
Affinity Capture-MS Homo sapiens
24 STX4 6810
Proximity Label-MS Homo sapiens
25 MBNL1 4154
Affinity Capture-MS Homo sapiens
26 SOX2  
Affinity Capture-MS Homo sapiens
27 DNAJC5 80331
Proximity Label-MS Homo sapiens
28 MAPK6  
Affinity Capture-MS Homo sapiens
29 EPHA2 1969
Proximity Label-MS Homo sapiens
30 B3GAT1  
Proximity Label-MS Homo sapiens
31 CDH1 999
Proximity Label-MS Homo sapiens
32 EZH2  
Affinity Capture-MS Homo sapiens
33 FANCG 2189
Affinity Capture-MS Homo sapiens
34 PKP4 8502
Two-hybrid Homo sapiens
35 PIGT 51604
Affinity Capture-MS Homo sapiens
36 PINK1  
Affinity Capture-MS Homo sapiens
37 SSUH2  
Affinity Capture-MS Homo sapiens
38 ALDH3A1 218
Affinity Capture-MS Homo sapiens
39 RHOB 388
Proximity Label-MS Homo sapiens
40 DDX39B 7919
Affinity Capture-MS Homo sapiens
41 CAV1 857
Proximity Label-MS Homo sapiens
42 ELOVL5 60481
Proximity Label-MS Homo sapiens
43 MCM2 4171
Affinity Capture-MS Homo sapiens
44 CYLD  
Affinity Capture-MS Homo sapiens
45 CUL4A 8451
Affinity Capture-MS Homo sapiens
46 ITGA8 8516
Affinity Capture-MS Homo sapiens
47 PKP3 11187
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
48 UCHL5 51377
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which DSC3 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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