Gene description for PKP4
Gene name plakophilin 4
Gene symbol PKP4
Other names/aliases p0071
Species Homo sapiens
 Database cross references - PKP4
ExoCarta ExoCarta_8502
Vesiclepedia VP_8502
Entrez Gene 8502
HGNC 9026
MIM 604276
UniProt Q99569  
 PKP4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PKP4
Molecular Function
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 IBA
Biological Process
    cell-cell junction assembly GO:0007043 ISS
    cell-cell signaling GO:0007267 NAS
    regulation of cell adhesion GO:0030155 NAS
    positive regulation of cytokinesis GO:0032467 IMP
    positive regulation of GTPase activity GO:0043547 IDA
    cell-cell adhesion GO:0098609 IBA
Subcellular Localization
    spindle pole GO:0000922 IDA
    cornified envelope GO:0001533 TAS
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoskeleton GO:0005856 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IBA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    postsynaptic density GO:0014069 IEA
    cell junction GO:0030054 IDA
    desmosome GO:0030057 IDA
    midbody GO:0030496 IDA
    midbody GO:0030496 IDA
    cell-cell contact zone GO:0044291 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    spindle midzone GO:0051233 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified PKP4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PKP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 CCL3  
Affinity Capture-MS Homo sapiens
4 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 PFDN1 5201
Affinity Capture-MS Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 PAXIP1  
Two-hybrid Homo sapiens
9 MARCKS 4082
Proximity Label-MS Homo sapiens
10 Cdh1 12550
Affinity Capture-MS Mus musculus
11 GJA1 2697
Proximity Label-MS Homo sapiens
12 RACGAP1 29127
Affinity Capture-MS Homo sapiens
13 CDH5  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TRIM66  
Affinity Capture-MS Homo sapiens
15 APBA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 STX4 6810
Proximity Label-MS Homo sapiens
17 SOX2  
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 TAX1BP3 30851
Affinity Capture-MS Homo sapiens
20 FERMT3 83706
Affinity Capture-MS Homo sapiens
21 MLLT4 4301
Proximity Label-MS Homo sapiens
22 MCM2 4171
Affinity Capture-MS Homo sapiens
23 CDH2 1000
Two-hybrid Homo sapiens
24 SCRIB 23513
Affinity Capture-Western Homo sapiens
25 BRPF3  
Affinity Capture-MS Homo sapiens
26 STX6 10228
Proximity Label-MS Homo sapiens
27 BACE2 25825
Affinity Capture-MS Homo sapiens
28 GPRC5B 51704
Affinity Capture-MS Homo sapiens
29 GTSE1 51512
Affinity Capture-MS Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 USP7 7874
Affinity Capture-MS Homo sapiens
32 EBAG9 9166
Proximity Label-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 NINL  
Proximity Label-MS Homo sapiens
35 CAMK2A 815
Affinity Capture-MS Homo sapiens
36 CXADR 1525
Proximity Label-MS Homo sapiens
37 ARMC8 25852
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 LYN 4067
Proximity Label-MS Homo sapiens
39 Sec24c  
Affinity Capture-MS Mus musculus
40 MCAM 4162
Proximity Label-MS Homo sapiens
41 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 DNAJC5 80331
Proximity Label-MS Homo sapiens
43 UBASH3B 84959
Two-hybrid Homo sapiens
44 CDH1 999
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
45 CDH8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PARD3 56288
Proximity Label-MS Homo sapiens
47 DIRAS3  
Proximity Label-MS Homo sapiens
48 Prkacb 18749
Affinity Capture-MS Mus musculus
49 DTX2 113878
Proximity Label-MS Homo sapiens
50 CTNNB1 1499
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MOV10 4343
Affinity Capture-RNA Homo sapiens
52 CLEC11A 6320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
54 APBA1  
Affinity Capture-MS Homo sapiens
55 CTNNA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MRAP2  
Affinity Capture-MS Homo sapiens
57 CDH12  
Affinity Capture-MS Homo sapiens
58 TMEM52B 120939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 OGT 8473
Reconstituted Complex Homo sapiens
60 EFNA4  
Affinity Capture-MS Homo sapiens
61 PSEN1 5663
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
62 KIF20A 10112
Affinity Capture-MS Homo sapiens
63 USP15 9958
Affinity Capture-MS Homo sapiens
64 ERBB2IP 55914
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
65 EPB41L4A 64097
Proximity Label-MS Homo sapiens
66 LAMP1 3916
Proximity Label-MS Homo sapiens
67 CLEC4A  
Affinity Capture-MS Homo sapiens
68 OSGEP 55644
Two-hybrid Homo sapiens
69 ARF6 382
Proximity Label-MS Homo sapiens
70 SNX27 81609
Affinity Capture-MS Homo sapiens
71 Evpl  
Affinity Capture-MS Mus musculus
72 NXF1 10482
Affinity Capture-RNA Homo sapiens
73 FLRT1  
Affinity Capture-MS Homo sapiens
74 LZTS2 84445
Two-hybrid Homo sapiens
75 KCNE3  
Affinity Capture-MS Homo sapiens
76 FGFR2 2263
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 DSC3 1825
Two-hybrid Homo sapiens
78 ABTB2 25841
Affinity Capture-MS Homo sapiens
79 NIN 51199
Proximity Label-MS Homo sapiens
80 SAV1  
Proximity Label-MS Homo sapiens
81 ATPAF2 91647
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
82 MATR3 9782
Affinity Capture-MS Homo sapiens
83 RHOB 388
Proximity Label-MS Homo sapiens
84 CLTC 1213
Affinity Capture-MS Homo sapiens
85 CTNNA1 1495
Proximity Label-MS Homo sapiens
86 Mad2l1 56150
Affinity Capture-MS Mus musculus
87 KCNJ5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 FLOT1 10211
Proximity Label-MS Homo sapiens
89 DSP 1832
Two-hybrid Homo sapiens
90 NES 10763
Affinity Capture-MS Homo sapiens
91 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
92 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 LAMTOR1 55004
Proximity Label-MS Homo sapiens
94 PDZD2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
95 CCDC85A  
Affinity Capture-MS Homo sapiens
96 Akap8  
Affinity Capture-MS Mus musculus
97 AMHR2  
Affinity Capture-MS Homo sapiens
98 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 RYK 6259
Affinity Capture-MS Homo sapiens
100 LCK 3932
Proximity Label-MS Homo sapiens
101 CAPZA2 830
Affinity Capture-MS Homo sapiens
102 LATS2 26524
Proximity Label-MS Homo sapiens
103 C11orf52 91894
Proximity Label-MS Homo sapiens
104 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 LATS1  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
106 Tuba3a 22144
Affinity Capture-MS Mus musculus
107 RAB2A 5862
Proximity Label-MS Homo sapiens
108 TRIM23 373
Two-hybrid Homo sapiens
109 C16orf54 283897
Affinity Capture-MS Homo sapiens
110 RAB11A 8766
Proximity Label-MS Homo sapiens
111 EPHA2 1969
Proximity Label-MS Homo sapiens
112 PTPRK 5796
Proximity Label-MS Homo sapiens
113 KRAS 3845
Proximity Label-MS Homo sapiens
114 CCDC96  
Affinity Capture-MS Homo sapiens
115 CAV1 857
Proximity Label-MS Homo sapiens
116 LNX1  
Two-hybrid Homo sapiens
View the network image/svg+xml



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