ExoCarta: Gene summary

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Gene description for OCLN
Gene name	occludin
Gene symbol	OCLN
Other names/aliases	BLCPMG PPP1R115
Species	Homo sapiens
Database cross references - OCLN
ExoCarta	ExoCarta_100506658
Vesiclepedia	VP_100506658
Entrez Gene	100506658
HGNC	8104
MIM	602876
UniProt	Q16625
OCLN identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Colorectal cancer cells	23161513
Colorectal cancer cells	34887515
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Prostate cancer cells	25844599

Gene ontology annotations for OCLN

Molecular Function
	protein binding	GO:0005515	IPI
	protein domain specific binding	GO:0019904	IPI
Biological Process
	negative regulation of protein phosphorylation	GO:0001933	IMP
	positive regulation of lamellipodium assembly	GO:0010592	ISS
	positive regulation of gene expression	GO:0010628	IMP
	negative regulation of gene expression	GO:0010629	IMP
	regulation of D-glucose transmembrane transport	GO:0010827	IMP
	positive regulation of microtubule polymerization	GO:0031116	ISS
	maintenance of blood-brain barrier	GO:0035633	NAS
	cell-cell junction organization	GO:0045216	IMP
	positive regulation of D-glucose import	GO:0046326	IMP
	protein-containing complex assembly	GO:0065003	TAS
	bicellular tight junction assembly	GO:0070830	IBA
	bicellular tight junction assembly	GO:0070830	IMP
	positive regulation of wound healing	GO:0090303	ISS
	protein localization to cell leading edge	GO:1902463	ISS
	positive regulation of blood-brain barrier permeability	GO:1905605	IMP
Subcellular Localization
	lysosomal membrane	GO:0005765	IDA
	plasma membrane	GO:0005886	IDA
	plasma membrane	GO:0005886	TAS
	cell-cell junction	GO:0005911	IDA
	bicellular tight junction	GO:0005923	IBA
	bicellular tight junction	GO:0005923	IDA
	apical plasma membrane	GO:0016324	IBA
	apicolateral plasma membrane	GO:0016327	IEA
	cell junction	GO:0030054	IDA
	cell junction	GO:0030054	TAS
	endocytic vesicle	GO:0030139	IEA
	cell leading edge	GO:0031252	ISS
	cytoplasmic vesicle	GO:0031410	IBA
	cytoplasmic vesicle	GO:0031410	IDA
	protein-containing complex	GO:0032991	IDA
	tight junction	GO:0070160	IDA
	tight junction	GO:0070160	IMP

Experiment description of studies that identified OCLN in exosomes

Experiment ID

207

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DKO-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

208

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

Dks-8

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

209

MISEV standards
✘	Biophysical techniques
✔ TSG101\|HSP70\|FLOT1	Enriched markers
✔ VDAC	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23161513

Organism

Homo sapiens

Experiment description

Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.

Authors

"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."

Journal name

Mol Cell Proteomics

Publication year

2012

Sample

Colorectal cancer cells

Sample name

DLD-1

Isolation/purification methods

Differential centrifugation
Filtration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

275

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|RAB5A\|CD9\|CD82\|CD63\|CD81	Enriched markers
✔ AIF	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25844599

Organism

Homo sapiens

Experiment description

Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.

Authors

"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."

Journal name

Oncotarget

Publication year

2015

Sample

Prostate cancer cells

Sample name

DU145 - Docetaxel sensitive

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.12-1.19 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry/Flow cytometry/Western blotting

Protein-protein interactions for OCLN

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

ITGB1

3688

Proximity Label-MS

Homo sapiens

GJD3

Proximity Label-MS

Homo sapiens

ARHGAP1

392

Proximity Label-MS

Homo sapiens

WDR6

11180

Proximity Label-MS

Homo sapiens

UTRN

7402

Proximity Label-MS

Homo sapiens

RASSF8

11228

Proximity Label-MS

Homo sapiens

ATG9A

79065

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

LLGL1

3996

Proximity Label-MS

Homo sapiens

PKP2

5318

Proximity Label-MS

Homo sapiens

CSNK1A1

1452

Reconstituted Complex

Homo sapiens

JPH1

56704

Proximity Label-MS

Homo sapiens

SYNE4

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens

C9orf78

51759

Affinity Capture-MS

Homo sapiens

C1orf226

Proximity Label-MS

Homo sapiens

MAP4K4

9448

Proximity Label-MS

Homo sapiens

LRRN4CL

221091

Affinity Capture-MS

Homo sapiens

MON2

23041

Proximity Label-MS

Homo sapiens

CFAP97

Proximity Label-MS

Homo sapiens

PLCH1

Proximity Label-MS

Homo sapiens

UNC5B

219699

Proximity Label-MS

Homo sapiens

ABI1

10006

Proximity Label-MS

Homo sapiens

MAN1A1

4121

Affinity Capture-MS

Homo sapiens

GPX8

493869

Two-hybrid

Homo sapiens

SLC12A7

10723

Proximity Label-MS

Homo sapiens

MPZL2

10205

Affinity Capture-MS

Homo sapiens

TMEM87A

25963

Proximity Label-MS

Homo sapiens

KIF14

9928

Proximity Label-MS

Homo sapiens

NOTCH2

4853

Proximity Label-MS

Homo sapiens

C19orf38

Affinity Capture-MS

Homo sapiens

SH3BP4

23677

Proximity Label-MS

Homo sapiens

LCTL

Affinity Capture-MS

Homo sapiens

EFR3B

Proximity Label-MS

Homo sapiens

PDXDC1

23042

Proximity Label-MS

Homo sapiens

COMTD1

118881

Affinity Capture-MS

Homo sapiens

CDH1

999

Proximity Label-MS

Homo sapiens

TANC1

85461

Proximity Label-MS

Homo sapiens

BACE2

25825

Affinity Capture-MS

Homo sapiens

GORASP2

26003

Proximity Label-MS

Homo sapiens

GJB1

2705

Affinity Capture-Western

Homo sapiens

CSNK1E

1454

Affinity Capture-Western		Homo sapiens
Biochemical Activity		Homo sapiens
Two-hybrid		Homo sapiens
Reconstituted Complex		Homo sapiens
Co-localization		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MPP7

143098

Proximity Label-MS

Homo sapiens

FLOT2

2319

Proximity Label-MS

Homo sapiens

PICALM

8301

Proximity Label-MS

Homo sapiens

TMEM171

Affinity Capture-MS

Homo sapiens

IQGAP2

10788

Proximity Label-MS

Homo sapiens

DST

667

Proximity Label-MS

Homo sapiens

PALM2

Proximity Label-MS

Homo sapiens

EPB41L1

2036

Proximity Label-MS

Homo sapiens

ARHGAP32

Proximity Label-MS

Homo sapiens

EPS15

2060

Affinity Capture-Western

Homo sapiens

FAM129B

64855

Proximity Label-MS

Homo sapiens

SIGLECL1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SPECC1

92521

Proximity Label-MS

Homo sapiens

CDCA3

83461

Proximity Label-MS

Homo sapiens

NBEA

Proximity Label-MS

Homo sapiens

DUOXA2

Affinity Capture-MS

Homo sapiens

ADD3

120

Proximity Label-MS

Homo sapiens

ERBB2IP

55914

Proximity Label-MS

Homo sapiens

DLG1

1739

Proximity Label-MS

Homo sapiens

LMTK2

22853

Proximity Label-MS

Homo sapiens

KLRG2

346689

Affinity Capture-MS

Homo sapiens

KCNB2

Proximity Label-MS

Homo sapiens

SNX9

51429

Proximity Label-MS

Homo sapiens

TJP2

9414

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Proximity Label-MS		Homo sapiens

ABCC1

4363

Proximity Label-MS

Homo sapiens

ZER1

10444

Affinity Capture-MS

Homo sapiens

DZIP1

Proximity Label-MS

Homo sapiens

ENTPD2

954

Affinity Capture-MS

Homo sapiens

MAP4K3

8491

Proximity Label-MS

Homo sapiens

CTNNA1

1495

Proximity Label-MS

Homo sapiens

KIDINS220

57498

Proximity Label-MS

Homo sapiens

EFNB2

1948

Proximity Label-MS

Homo sapiens

PDE8A

5151

Proximity Label-MS

Homo sapiens

GOLGB1

2804

Proximity Label-MS

Homo sapiens

SLC4A2

6522

Proximity Label-MS

Homo sapiens

GPRIN3

Proximity Label-MS

Homo sapiens

DVL1

1855

Proximity Label-MS

Homo sapiens

TP53BP2

Proximity Label-MS

Homo sapiens

PSD3

23362

Proximity Label-MS

Homo sapiens

STK16

8576

Affinity Capture-MS

Homo sapiens

SLC18A1

Affinity Capture-MS

Homo sapiens

TGFBR2

7048

Affinity Capture-Western

Homo sapiens

RAB11FIP5

26056

Proximity Label-MS

Homo sapiens

SCYL3

57147

Proximity Label-MS

Homo sapiens

MACF1

23499

Proximity Label-MS

Homo sapiens

CD1B

910

Affinity Capture-MS

Homo sapiens

LCK

3932

Proximity Label-MS

Homo sapiens

SPTBN2

6712

Proximity Label-MS

Homo sapiens

PTPN2

5771

Biochemical Activity

Homo sapiens

DLK1

Affinity Capture-MS

Homo sapiens

SIRT1

Proximity Label-MS

Homo sapiens

TRIP11

9321

Proximity Label-MS

Homo sapiens

ANKRD26

22852

Proximity Label-MS

Homo sapiens

RAB11FIP1

80223

Proximity Label-MS

Homo sapiens

RAB2A

5862

Proximity Label-MS

Homo sapiens

PHACTR4

65979

Proximity Label-MS

Homo sapiens

GOLGA5

9950

Proximity Label-MS

Homo sapiens

HBEGF

1839

Affinity Capture-MS

Homo sapiens

RAB11A

8766

Proximity Label-MS

Homo sapiens

100

PLEKHA7

144100

Proximity Label-MS

Homo sapiens

101

EPHA2

1969

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

102

SNW1

22938

Two-hybrid

Homo sapiens

103

RAB5C

5878

Proximity Label-MS

Homo sapiens

104

ADGRE5

976

Affinity Capture-MS

Homo sapiens

105

VANGL1

81839

Proximity Label-MS

Homo sapiens

106

UNC13B

10497

Proximity Label-MS

Homo sapiens

107

CTNND1

1500

Proximity Label-MS

Homo sapiens

108

SRPRB

58477

Affinity Capture-MS

Homo sapiens

109

USP6NL

9712

Proximity Label-MS

Homo sapiens

110

CCSER2

54462

Proximity Label-MS

Homo sapiens

111

NDRG1

10397

Proximity Label-MS

Homo sapiens

112

CHRNA4

Affinity Capture-MS

Homo sapiens

113

ARHGAP39

Proximity Label-MS

Homo sapiens

114

Tjp1

Reconstituted Complex

Mus musculus

115

LAMP3

Proximity Label-MS

Homo sapiens

116

NOS1AP

Proximity Label-MS

Homo sapiens

117

GJA1

2697

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

118

C1orf198

84886

Proximity Label-MS

Homo sapiens

119

APC

Proximity Label-MS

Homo sapiens

120

NUMBL

9253

Proximity Label-MS

Homo sapiens

121

PACSIN2

11252

Proximity Label-MS

Homo sapiens

122

DTNA

Proximity Label-MS

Homo sapiens

123

ARF3

377

Proximity Label-MS

Homo sapiens

124

PHLDB1

23187

Proximity Label-MS

Homo sapiens

125

FMN2

Proximity Label-MS

Homo sapiens

126

VAMP2

6844

Proximity Label-MS

Homo sapiens

127

IFNGR1

3459

Proximity Label-MS

Homo sapiens

128

FFAR1

Affinity Capture-MS

Homo sapiens

129

TGFBR1

7046

Affinity Capture-Western		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens

130

VANGL2

Proximity Label-MS

Homo sapiens

131

CTLA4

Affinity Capture-MS

Homo sapiens

132

ADAM9

8754

Affinity Capture-MS

Homo sapiens

133

TNIK

23043

Proximity Label-MS

Homo sapiens

134

MAP4K5

11183

Proximity Label-MS

Homo sapiens

135

TMEM182

Affinity Capture-MS

Homo sapiens

136

PCNXL3

399909

Proximity Label-MS

Homo sapiens

137

STEAP3

55240

Proximity Label-MS

Homo sapiens

138

PTPN14

5784

Proximity Label-MS

Homo sapiens

139

PALM

5064

Proximity Label-MS

Homo sapiens

140

EBAG9

9166

Proximity Label-MS

Homo sapiens

141

LMAN1

3998

Proximity Label-MS

Homo sapiens

142

GOPC

57120

Proximity Label-MS

Homo sapiens

143

STAM2

10254

Proximity Label-MS

Homo sapiens

144

SDK2

54549

Proximity Label-MS

Homo sapiens

145

SLC1A5

6510

Proximity Label-MS

Homo sapiens

146

FBLN1

2192

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

147

RAI14

26064

Proximity Label-MS

Homo sapiens

148

C2CD4C

Proximity Label-MS

Homo sapiens

149

NHS

4810

Proximity Label-MS

Homo sapiens

150

LYN

4067

Proximity Label-MS

Homo sapiens

151

ADD2

119

Proximity Label-MS

Homo sapiens

152

SIPA1L2

Proximity Label-MS

Homo sapiens

153

TRPM7

54822

Proximity Label-MS

Homo sapiens

154

LAMP1

3916

Proximity Label-MS

Homo sapiens

155

JAG2

Proximity Label-MS

Homo sapiens

156

PREB

10113

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

157

FAM171A1

221061

Proximity Label-MS

Homo sapiens

158

DNAJC5B

Proximity Label-MS

Homo sapiens

159

TMX1

81542

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

160

MINK1

50488

Proximity Label-MS

Homo sapiens

161

MARVELD2

153562

Proximity Label-MS

Homo sapiens

162

SLC19A1

6573

Proximity Label-MS

Homo sapiens

163

RAB5A

5868

Proximity Label-MS

Homo sapiens

164

TMPRSS11B

132724

Affinity Capture-MS

Homo sapiens

165

TNFSF8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

166

ADCY9

115

Proximity Label-MS

Homo sapiens

167

CDC42EP1

11135

Proximity Label-MS

Homo sapiens

168

TJP1

7082

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens
Proximity Label-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens