Gene description for SRPRB
Gene name signal recognition particle receptor, B subunit
Gene symbol SRPRB
Other names/aliases APMCF1
Species Homo sapiens
 Database cross references - SRPRB
ExoCarta ExoCarta_58477
Entrez Gene 58477
HGNC 24085
UniProt Q9Y5M8  
 SRPRB identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SRPRB
Molecular Function
    GTP binding GO:0005525 IEA
Biological Process
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
    cellular protein metabolic process GO:0044267 TAS
    small GTPase mediated signal transduction GO:0007264 IEA
    IRE1-mediated unfolded protein response GO:0036498 TAS
    gene expression GO:0010467 TAS
    translation GO:0006412 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
    cytoplasmic microtubule GO:0005881 IDA
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified SRPRB in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRPRB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CASP4  
Affinity Capture-MS Homo sapiens
2 PTP4A3  
Affinity Capture-MS Homo sapiens
3 UQCRC1 7384
Affinity Capture-MS Homo sapiens
4 TSC22D1  
Affinity Capture-MS Homo sapiens
5 NDN  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SRPRB is involved
PathwayEvidenceSource
SRP-dependent cotranslational protein targeting to membrane TAS Reactome
XBP1(S) activates chaperone genes TAS Reactome





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