Gene description for ACAA1
Gene name acetyl-CoA acyltransferase 1
Gene symbol ACAA1
Other names/aliases ACAA
PTHIO
THIO
Species Homo sapiens
 Database cross references - ACAA1
ExoCarta ExoCarta_30
Entrez Gene 30
HGNC 82
MIM 604054
UniProt P09110  
 ACAA1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for ACAA1
Molecular Function
    protein binding GO:0005515 IPI
    palmitoyl-CoA oxidase activity GO:0016401 IMP
    acetyl-CoA C-acyltransferase activity GO:0003988 IEA
Biological Process
    fatty acid beta-oxidation GO:0006635 IMP
    cellular lipid metabolic process GO:0044255 TAS
    unsaturated fatty acid metabolic process GO:0033559 TAS
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 TAS
    small molecule metabolic process GO:0044281 TAS
    alpha-linolenic acid metabolic process GO:0036109 TAS
    very long-chain fatty acid metabolic process GO:0000038 IMP
    bile acid metabolic process GO:0008206 IMP
Subcellular Localization
    peroxisome GO:0005777 IDA
    peroxisomal matrix GO:0005782 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified ACAA1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACAA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ACAA1 is involved
PathwayEvidenceSource
alpha-linolenic acid (ALA) metabolism TAS Reactome
Beta-oxidation of very long chain fatty acids TAS Reactome





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