Gene description for PEX5
Gene name peroxisomal biogenesis factor 5
Gene symbol PEX5
Other names/aliases PBD2A
PBD2B
PTS1-BP
PTS1R
PXR1
Species Homo sapiens
 Database cross references - PEX5
ExoCarta ExoCarta_5830
Vesiclepedia VP_5830
Entrez Gene 5830
HGNC 9719
MIM 600414
UniProt P50542  
 PEX5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PEX5
Molecular Function
    peroxisome targeting sequence binding GO:0000268 IDA
    peroxisome matrix targeting signal-1 binding GO:0005052 IBA
    peroxisome matrix targeting signal-1 binding GO:0005052 IDA
    peroxisome matrix targeting signal-1 binding GO:0005052 IMP
    peroxisome matrix targeting signal-1 binding GO:0005052 IPI
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    small GTPase binding GO:0031267 IPI
    peroxisome membrane targeting sequence binding GO:0033328 IPI
    protein carrier chaperone GO:0140597 IDA
    protein carrier chaperone GO:0140597 IMP
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IEA
    pexophagy GO:0000425 IDA
    neuron migration GO:0001764 IEA
    protein targeting to peroxisome GO:0006625 IDA
    protein targeting to peroxisome GO:0006625 IMP
    fatty acid beta-oxidation GO:0006635 IEA
    mitochondrial membrane organization GO:0007006 IEA
    endoplasmic reticulum organization GO:0007029 IEA
    protein import into peroxisome matrix GO:0016558 IDA
    protein import into peroxisome matrix GO:0016558 IGI
    protein import into peroxisome matrix GO:0016558 IMP
    protein import into peroxisome matrix GO:0016558 NAS
    protein import into peroxisome matrix, docking GO:0016560 IBA
    protein import into peroxisome matrix, docking GO:0016560 IDA
    protein import into peroxisome matrix, translocation GO:0016561 IDA
    protein import into peroxisome matrix, receptor recycling GO:0016562 IDA
    cerebral cortex cell migration GO:0021795 IEA
    cerebral cortex neuron differentiation GO:0021895 IEA
    negative regulation of protein-containing complex assembly GO:0031333 IDA
    cellular response to reactive oxygen species GO:0034614 IDA
    positive regulation of multicellular organism growth GO:0040018 IEA
    protein import into peroxisome matrix, substrate release GO:0044721 IC
    protein import into peroxisome matrix, substrate release GO:0044721 IDA
    protein import into peroxisome matrix, substrate release GO:0044721 ISS
    protein import into peroxisome membrane GO:0045046 IMP
    cell development GO:0048468 IEA
    neuromuscular process GO:0050905 IEA
    protein tetramerization GO:0051262 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IEA
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 TAS
    peroxisomal matrix GO:0005782 IC
    peroxisomal matrix GO:0005782 IDA
    peroxisomal matrix GO:0005782 IDA
    peroxisomal matrix GO:0005782 IMP
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified PEX5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PEX5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
Two-hybrid Homo sapiens
2 PEX13  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
3 PRR13  
Two-hybrid Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
5 HOOK1  
Proximity Label-MS Homo sapiens
6 MTCH1 23787
Proximity Label-MS Homo sapiens
7 IDH1 3417
Protein-peptide Homo sapiens
8 NUDT7  
Protein-peptide Homo sapiens
9 FBXO7 25793
Affinity Capture-MS Homo sapiens
10 PEX7  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
11 LDHB 3945
Two-hybrid Homo sapiens
12 HOXA7  
Two-hybrid Homo sapiens
13 ACAA1 30
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 S100A6 6277
Two-hybrid Homo sapiens
15 RAMP1  
Affinity Capture-MS Homo sapiens
16 GSTK1 373156
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
17 BRCA1 672
Two-hybrid Homo sapiens
18 DAO  
Protein-peptide Homo sapiens
19 TRIM33 51592
Affinity Capture-MS Homo sapiens
20 USP9X 8239
Biochemical Activity Homo sapiens
21 MAD2L1BP  
Affinity Capture-MS Homo sapiens
22 RPL14 9045
Two-hybrid Homo sapiens
23 ATM 472
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 PTPN1 5770
Proximity Label-MS Homo sapiens
25 PHYH 5264
Affinity Capture-MS Homo sapiens
26 CAPRIN2  
Two-hybrid Homo sapiens
27 LONP2 83752
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
28 TMEM154  
Affinity Capture-MS Homo sapiens
29 FIS1 51024
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
30 DECR2 26063
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
31 FAM57A  
Affinity Capture-MS Homo sapiens
32 TIMMDC1  
Two-hybrid Homo sapiens
33 CCDC14  
Two-hybrid Homo sapiens
34 NUDT3 11165
Two-hybrid Homo sapiens
35 SLC27A2 11001
Protein-peptide Homo sapiens
36 TOMM7 54543
Two-hybrid Homo sapiens
37 MARCH5  
Proximity Label-MS Homo sapiens
38 TINF2  
Two-hybrid Homo sapiens
39 DDO  
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
40 ACOT8 10005
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
41 EXD2  
Proximity Label-MS Homo sapiens
42 MAP3K6  
Affinity Capture-MS Homo sapiens
43 POT1  
Two-hybrid Homo sapiens
44 USP2  
Biochemical Activity Homo sapiens
45 MAVS 57506
Proximity Label-MS Homo sapiens
46 HACL1 26061
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
47 HSD17B4 3295
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
48 NPAS1  
Affinity Capture-MS Homo sapiens
49 PSAP 5660
Two-hybrid Homo sapiens
50 VWA8 23078
Proximity Label-MS Homo sapiens
51 GNPAT 8443
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
52 FAS 355
Proximity Label-MS Homo sapiens
53 CACNB3  
Affinity Capture-MS Homo sapiens
54 ZADH2 284273
Affinity Capture-MS Homo sapiens
55 GARS 2617
Affinity Capture-MS Homo sapiens
56 KRT8 3856
Proximity Label-MS Homo sapiens
57 CROT 54677
Protein-peptide Homo sapiens
58 GLMN 11146
Two-hybrid Homo sapiens
59 MTCH2 23788
Proximity Label-MS Homo sapiens
60 IDE 3416
Protein-peptide Homo sapiens
61 PRDX5 25824
Protein-peptide Homo sapiens
62 PEX12  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
63 SNAP91 9892
Affinity Capture-MS Homo sapiens
64 B4GALT2  
Affinity Capture-MS Homo sapiens
65 ACOT2 10965
Protein-peptide Homo sapiens
66 AMACR 23600
Protein-peptide Homo sapiens
67 IFNG 3458
Affinity Capture-MS Homo sapiens
68 PEX5L  
Negative Genetic Homo sapiens
69 HLX  
Affinity Capture-MS Homo sapiens
70 KIAA1683  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 Sidt2  
Affinity Capture-MS Mus musculus
72 MYH9 4627
Affinity Capture-MS Homo sapiens
73 CGREF1 10669
Affinity Capture-MS Homo sapiens
74 GDPD5 81544
Two-hybrid Homo sapiens
75 ENTPD1 953
Affinity Capture-MS Homo sapiens
76 PEX14 5195
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
77 NOS2  
Protein-peptide Homo sapiens
78 HMGCL 3155
Protein-peptide Homo sapiens
79 ACOX2  
Protein-peptide Homo sapiens
80 ACOT4  
Protein-peptide Homo sapiens
81 HCN1  
Affinity Capture-MS Homo sapiens
82 TECR 9524
Affinity Capture-MS Homo sapiens
83 AQP12B  
Affinity Capture-MS Homo sapiens
84 ACAD11 84129
Affinity Capture-MS Homo sapiens
85 AGPS 8540
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 RANBP6 26953
Co-fractionation Homo sapiens
87 RHOT2 89941
Proximity Label-MS Homo sapiens
88 FGFR1OP  
Affinity Capture-MS Homo sapiens
89 CD93  
Affinity Capture-MS Homo sapiens
90 RMDN3 55177
Proximity Label-MS Homo sapiens
91 HAO2  
Protein-peptide Homo sapiens
92 ACD  
Two-hybrid Homo sapiens
93 CAT 847
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
94 ECH1 1891
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
95 FAM3D  
Affinity Capture-MS Homo sapiens
96 ZFAND3  
Two-hybrid Homo sapiens
97 CUL3 8452
Affinity Capture-MS Homo sapiens
98 XPO1 7514
Affinity Capture-MS Homo sapiens
99 SDHA 6389
Affinity Capture-MS Homo sapiens
100 C11orf49  
Affinity Capture-MS Homo sapiens
101 SSX3  
Affinity Capture-MS Homo sapiens
102 MLYCD 23417
Protein-peptide Homo sapiens
103 CRELD1 78987
Affinity Capture-MS Homo sapiens
104 ERLEC1 27248
Affinity Capture-MS Homo sapiens
105 SYTL5 94122
Affinity Capture-MS Homo sapiens
106 PEX10  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
107 FKBP8 23770
Proximity Label-MS Homo sapiens
108 DHRS4 10901
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
109 TYSND1  
Affinity Capture-MS Homo sapiens
110 DUSP16  
Affinity Capture-MS Homo sapiens
111 OCIAD1 54940
Proximity Label-MS Homo sapiens
112 NXF1 10482
Affinity Capture-RNA Homo sapiens
113 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 ECI2 10455
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
115 CRAT 1384
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
116 RPA3 6119
Proximity Label-MS Homo sapiens
117 GOLGA1  
Proximity Label-MS Homo sapiens
118 ACOX3 8310
Protein-peptide Homo sapiens
119 HSPA1A 3303
Affinity Capture-MS Homo sapiens
120 PIPOX  
Protein-peptide Homo sapiens
121 TGFA  
Affinity Capture-MS Homo sapiens
122 TM6SF1  
Two-hybrid Homo sapiens
123 PEX2  
Affinity Capture-MS Homo sapiens
124 SCRN2 90507
Two-hybrid Homo sapiens
125 PDZK1 5174
Two-hybrid Homo sapiens
126 PEX3 8504
Proximity Label-MS Homo sapiens
127 SIRT3  
Two-hybrid Homo sapiens
128 FTSJ3 117246
Affinity Capture-MS Homo sapiens
129 HSDL2 84263
Affinity Capture-MS Homo sapiens
130 ECE2  
Affinity Capture-MS Homo sapiens
131 CCDC8  
Affinity Capture-MS Homo sapiens
132 ACBD5 91452
Proximity Label-MS Homo sapiens
133 EP400NL  
Two-hybrid Homo sapiens
134 Pex14  
Protein-peptide Rattus norvegicus
Reconstituted Complex Rattus norvegicus
135 CCND2  
Affinity Capture-MS Homo sapiens
136 ZNF772  
Two-hybrid Homo sapiens
137 EPHX2 2053
Protein-peptide Homo sapiens
138 SNUPN 10073
Two-hybrid Homo sapiens
139 BAAT  
Protein-peptide Homo sapiens
140 PAOX  
Protein-peptide Homo sapiens
141 EHHADH 1962
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
142 POLR3H  
Affinity Capture-MS Homo sapiens
143 SQSTM1 8878
Affinity Capture-Western Homo sapiens
144 MKRN3  
Two-hybrid Homo sapiens
145 DCTN2 10540
Proximity Label-MS Homo sapiens
146 HAO1  
Protein-peptide Homo sapiens
147 NUDT19 390916
Protein-peptide Homo sapiens
148 MAGEA9  
Affinity Capture-MS Homo sapiens
149 DDX58 23586
Affinity Capture-RNA Homo sapiens
150 PXMP2  
Proximity Label-MS Homo sapiens
151 MOV10 4343
Affinity Capture-RNA Homo sapiens
152 ACOX1 51
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
153 PECR 55825
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
154 PXN 5829
Proximity Label-MS Homo sapiens
155 KXD1 79036
Affinity Capture-MS Homo sapiens
156 STK11 6794
Affinity Capture-MS Homo sapiens
157 RNF145  
Affinity Capture-MS Homo sapiens
158 NEU2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 SCP2 6342
Protein-peptide Homo sapiens
160 CCDC96  
Affinity Capture-MS Homo sapiens
161 KIAA0408  
Affinity Capture-MS Homo sapiens
162 AGXT  
Protein-peptide Homo sapiens
163 MPV17 4358
Protein-peptide Homo sapiens
View the network image/svg+xml



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