Gene description for RPA3
Gene name replication protein A3, 14kDa
Gene symbol RPA3
Other names/aliases REPA3
Species Homo sapiens
 Database cross references - RPA3
ExoCarta ExoCarta_6119
Vesiclepedia VP_6119
Entrez Gene 6119
HGNC 10291
MIM 179837
UniProt P35244  
 RPA3 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RPA3
Molecular Function
    protein binding GO:0005515 IPI
    damaged DNA binding GO:0003684 IDA
    single-stranded DNA binding GO:0003697 IDA
Biological Process
    DNA replication GO:0006260 TAS
    error-free translesion synthesis GO:0070987 TAS
    base-excision repair GO:0006284 TAS
    nucleotide-excision repair GO:0006289 TAS
    DNA recombinase assembly GO:0000730 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    regulation of mitotic cell cycle GO:0007346 IEA
    double-strand break repair via homologous recombination GO:0000724 TAS
    mismatch repair GO:0006298 TAS
    DNA repair GO:0006281 TAS
    regulation of cellular response to heat GO:1900034 TAS
    cellular response to heat GO:0034605 TAS
    translesion synthesis GO:0019985 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    double-strand break repair GO:0006302 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    telomere maintenance via recombination GO:0000722 TAS
    error-prone translesion synthesis GO:0042276 TAS
    mitotic cell cycle GO:0000278 TAS
    telomere maintenance GO:0000723 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    regulation of cell proliferation GO:0042127 IEA
    nucleotide-excision repair, DNA damage removal GO:0000718 TAS
Subcellular Localization
    DNA replication factor A complex GO:0005662 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RPA3 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RPA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARP3 10039
Affinity Capture-MS Homo sapiens
2 TFE3  
Affinity Capture-MS Homo sapiens
3 RPA1 6117
Invitro Homo sapiens
4 CNBP 7555
Affinity Capture-MS Homo sapiens
5 ERCC6L 54821
Affinity Capture-MS Homo sapiens
6 PKIG  
Affinity Capture-MS Homo sapiens
7 PAK7  
Affinity Capture-MS Homo sapiens
8 TSC22D1 8848
Affinity Capture-MS Homo sapiens
9 RPA2 6118
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 GC20  
Affinity Capture-MS Homo sapiens
11 RPA3 6119
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 VHL  
Affinity Capture-MS Homo sapiens
13 MCM2 4171
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
14 TIMM13 26517
Affinity Capture-MS Homo sapiens
15 CTNNBIP1 56998
Affinity Capture-MS Homo sapiens
16 RPA4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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