Gene description for RPA3
Gene name replication protein A3, 14kDa
Gene symbol RPA3
Other names/aliases REPA3
Species Homo sapiens
 Database cross references - RPA3
ExoCarta ExoCarta_6119
Vesiclepedia VP_6119
Entrez Gene 6119
HGNC 10291
MIM 179837
UniProt P35244  
 RPA3 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RPA3
Molecular Function
    damaged DNA binding GO:0003684 IBA
    damaged DNA binding GO:0003684 IDA
    single-stranded DNA binding GO:0003697 IBA
    single-stranded DNA binding GO:0003697 IDA
    protein binding GO:0005515 IPI
Biological Process
    telomere maintenance GO:0000723 NAS
    telomere maintenance GO:0000723 TAS
    double-strand break repair via homologous recombination GO:0000724 IBA
    double-strand break repair via homologous recombination GO:0000724 IMP
    double-strand break repair via homologous recombination GO:0000724 NAS
    DNA replication GO:0006260 IBA
    DNA replication GO:0006260 IMP
    DNA replication GO:0006260 NAS
    DNA repair GO:0006281 IDA
    base-excision repair GO:0006284 IBA
    base-excision repair GO:0006284 IDA
    nucleotide-excision repair GO:0006289 IBA
    nucleotide-excision repair GO:0006289 IMP
    nucleotide-excision repair GO:0006289 NAS
    mismatch repair GO:0006298 IBA
    mismatch repair GO:0006298 IMP
    regulation of mitotic cell cycle GO:0007346 IEA
    regulation of cell population proliferation GO:0042127 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    DNA replication factor A complex GO:0005662 IBA
    DNA replication factor A complex GO:0005662 IDA
    DNA replication factor A complex GO:0005662 IPI
    site of double-strand break GO:0035861 IBA
 Experiment description of studies that identified RPA3 in exosomes
1
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RPA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Proximity Label-MS Homo sapiens
2 EIF3A 8661
Proximity Label-MS Homo sapiens
3 NPEPPS 9520
Proximity Label-MS Homo sapiens
4 HOOK1  
Proximity Label-MS Homo sapiens
5 CARS2 79587
Proximity Label-MS Homo sapiens
6 GMDS 2762
Proximity Label-MS Homo sapiens
7 C1orf27 54953
Proximity Label-MS Homo sapiens
8 EXOSC3 51010
Proximity Label-MS Homo sapiens
9 TBC1D4  
Proximity Label-MS Homo sapiens
10 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
11 TOP2B 7155
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
12 GDI1 2664
Proximity Label-MS Homo sapiens
13 KIAA2013 90231
Proximity Label-MS Homo sapiens
14 GLB1 2720
Proximity Label-MS Homo sapiens
15 CAP1 10487
Proximity Label-MS Homo sapiens
16 GAK 2580
Proximity Label-MS Homo sapiens
17 TUBB8 347688
Proximity Label-MS Homo sapiens
18 STK39 27347
Proximity Label-MS Homo sapiens
19 SMG7  
Proximity Label-MS Homo sapiens
20 NAA16 79612
Proximity Label-MS Homo sapiens
21 UBE3A 7337
Proximity Label-MS Homo sapiens
22 UBC 7316
Affinity Capture-MS Homo sapiens
23 THOC7 80145
Affinity Capture-MS Homo sapiens
24 VHL  
Affinity Capture-MS Homo sapiens
25 INTS1 26173
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
26 METTL5  
Proximity Label-MS Homo sapiens
27 GCN1L1 10985
Proximity Label-MS Homo sapiens
28 MRPS24 64951
Proximity Label-MS Homo sapiens
29 MAP1LC3B 81631
Proximity Label-MS Homo sapiens
30 PTRH2 51651
Proximity Label-MS Homo sapiens
31 MASTL  
Proximity Label-MS Homo sapiens
32 WDR76  
Affinity Capture-MS Homo sapiens
33 SMG9 56006
Proximity Label-MS Homo sapiens
34 CDK5 1020
Proximity Label-MS Homo sapiens
35 TRMU  
Proximity Label-MS Homo sapiens
36 GFPT1 2673
Proximity Label-MS Homo sapiens
37 RINT1 60561
Proximity Label-MS Homo sapiens
38 CIAPIN1 57019
Proximity Label-MS Homo sapiens
39 COX7A2L 9167
Proximity Label-MS Homo sapiens
40 ACTR3 10096
Proximity Label-MS Homo sapiens
41 POLR2A 5430
Proximity Label-MS Homo sapiens
42 BRCC3 79184
Proximity Label-MS Homo sapiens
43 ABCD3 5825
Proximity Label-MS Homo sapiens
44 ATAD5  
Proximity Label-MS Homo sapiens
45 RPS9 6203
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
46 SCARB1 949
Proximity Label-MS Homo sapiens
47 EPT1 85465
Proximity Label-MS Homo sapiens
48 KPRP 448834
Proximity Label-MS Homo sapiens
49 ZC3HAV1 56829
Proximity Label-MS Homo sapiens
50 SFN 2810
Proximity Label-MS Homo sapiens
51 NSFL1C 55968
Proximity Label-MS Homo sapiens
52 TUBB6 84617
Proximity Label-MS Homo sapiens
53 TFRC 7037
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
54 FAM120A 23196
Proximity Label-MS Homo sapiens
55 NTMT1 28989
Proximity Label-MS Homo sapiens
56 NOL11  
Proximity Label-MS Homo sapiens
57 EMC1 23065
Proximity Label-MS Homo sapiens
58 EIF3C 8663
Proximity Label-MS Homo sapiens
59 AGO2 27161
Proximity Label-MS Homo sapiens
60 UNK  
Affinity Capture-RNA Homo sapiens
61 AARS 16
Proximity Label-MS Homo sapiens
62 LDHB 3945
Proximity Label-MS Homo sapiens
63 TCOF1 6949
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
64 ANXA7 310
Proximity Label-MS Homo sapiens
65 TCP1 6950
Proximity Label-MS Homo sapiens
66 UTP14A 10813
Proximity Label-MS Homo sapiens
67 HINT1 3094
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
68 SRPRB 58477
Proximity Label-MS Homo sapiens
69 ACADVL 37
Proximity Label-MS Homo sapiens
70 CSTF2 1478
Proximity Label-MS Homo sapiens
71 BRE 9577
Proximity Label-MS Homo sapiens
72 TUFM 7284
Proximity Label-MS Homo sapiens
73 KIF13B 23303
Proximity Label-MS Homo sapiens
74 TUBB2B 347733
Proximity Label-MS Homo sapiens
75 ARG1 383
Affinity Capture-MS Homo sapiens
76 EEF1E1 9521
Proximity Label-MS Homo sapiens
77 MB21D2  
Proximity Label-MS Homo sapiens
78 SPR 6697
Proximity Label-MS Homo sapiens
79 NOC4L 79050
Proximity Label-MS Homo sapiens
80 NAA10 8260
Co-fractionation Homo sapiens
81 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
82 NOB1  
Proximity Label-MS Homo sapiens
83 RFC4 5984
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
84 CCDC22 28952
Proximity Label-MS Homo sapiens
85 ANAPC4 29945
Proximity Label-MS Homo sapiens
86 ALG12  
Proximity Label-MS Homo sapiens
87 CUL2 8453
Proximity Label-MS Homo sapiens
88 RRP1B 23076
Proximity Label-MS Homo sapiens
89 PEBP1 5037
Proximity Label-MS Homo sapiens
90 GTPBP4 23560
Proximity Label-MS Homo sapiens
91 IQGAP2 10788
Proximity Label-MS Homo sapiens
92 METTL1 4234
Proximity Label-MS Homo sapiens
93 CACYBP 27101
Proximity Label-MS Homo sapiens
94 PARP3 10039
Affinity Capture-MS Homo sapiens
95 ANLN 54443
Proximity Label-MS Homo sapiens
96 TRIP13 9319
Proximity Label-MS Homo sapiens
97 CISD1 55847
Proximity Label-MS Homo sapiens
98 HSPA5 3309
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
99 RBM39 9584
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
100 SLC2A1 6513
Proximity Label-MS Homo sapiens
101 SUGP1  
Proximity Label-MS Homo sapiens
102 SMARCE1 6605
Proximity Label-MS Homo sapiens
103 DCAF7 10238
Proximity Label-MS Homo sapiens
104 AHCYL1 10768
Proximity Label-MS Homo sapiens
105 LRRC59 55379
Proximity Label-MS Homo sapiens
106 LMNB1 4001
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
107 SCAMP1 9522
Proximity Label-MS Homo sapiens
108 LMO7 4008
Affinity Capture-MS Homo sapiens
109 NT5DC1 221294
Proximity Label-MS Homo sapiens
110 BCAP31 10134
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
111 MTAP 4507
Proximity Label-MS Homo sapiens
112 HSPA14 51182
Proximity Label-MS Homo sapiens
113 ZNF250  
Proximity Label-MS Homo sapiens
114 ATRIP  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
115 AAAS 8086
Proximity Label-MS Homo sapiens
116 EPRS 2058
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
117 EIF2S2 8894
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
118 ZC3H11A  
Proximity Label-MS Homo sapiens
119 NIP7 51388
Proximity Label-MS Homo sapiens
120 NTHL1  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
121 STRAP 11171
Proximity Label-MS Homo sapiens
122 PITPNA 5306
Proximity Label-MS Homo sapiens
123 CYP51A1 1595
Proximity Label-MS Homo sapiens
124 TUBA4A 7277
Affinity Capture-MS Homo sapiens
125 TJP2 9414
Proximity Label-MS Homo sapiens
126 CMSS1  
Proximity Label-MS Homo sapiens
127 ESD 2098
Proximity Label-MS Homo sapiens
128 GSTK1 373156
Proximity Label-MS Homo sapiens
129 TIGAR 57103
Proximity Label-MS Homo sapiens
130 SLC30A7 148867
Proximity Label-MS Homo sapiens
131 RFWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
132 STARD9 57519
Proximity Label-MS Homo sapiens
133 OCIAD1 54940
Proximity Label-MS Homo sapiens
134 AKT1 207
Proximity Label-MS Homo sapiens
135 UPF2 26019
Proximity Label-MS Homo sapiens
136 SEPT2 4735
Proximity Label-MS Homo sapiens
137 NT5C3B 115024
Proximity Label-MS Homo sapiens
138 RRAGC 64121
Proximity Label-MS Homo sapiens
139 NADK2 133686
Proximity Label-MS Homo sapiens
140 YIF1B 90522
Proximity Label-MS Homo sapiens
141 STUB1 10273
Proximity Label-MS Homo sapiens
142 HK2 3099
Proximity Label-MS Homo sapiens
143 DDX46 9879
Affinity Capture-MS Homo sapiens
144 NOSIP 51070
Proximity Label-MS Homo sapiens
145 YBX1 4904
Proximity Label-MS Homo sapiens
146 CHML  
Proximity Label-MS Homo sapiens
147 TUBGCP2 10844
Proximity Label-MS Homo sapiens
148 IDH3B 3420
Proximity Label-MS Homo sapiens
149 CADM1 23705
Proximity Label-MS Homo sapiens
150 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
151 RPL6 6128
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
152 RRS1 23212
Proximity Label-MS Homo sapiens
153 MACF1 23499
Proximity Label-MS Homo sapiens
154 MTA1 9112
Proximity Label-MS Homo sapiens
155 TCERG1 10915
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
156 LARS2 23395
Proximity Label-MS Homo sapiens
157 DCTN2 10540
Proximity Label-MS Homo sapiens
158 TTK 7272
Proximity Label-MS Homo sapiens
159 NUDT19 390916
Proximity Label-MS Homo sapiens
160 GTF2B 2959
Proximity Label-MS Homo sapiens
161 ELMO2 63916
Proximity Label-MS Homo sapiens
162 SSBP1 6742
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 PSMA1 5682
Proximity Label-MS Homo sapiens
164 RHEB 6009
Proximity Label-MS Homo sapiens
165 LARP7 51574
Proximity Label-MS Homo sapiens
166 CLASP1 23332
Proximity Label-MS Homo sapiens
167 EIF4B 1975
Proximity Label-MS Homo sapiens
168 DDX6 1656
Proximity Label-MS Homo sapiens
169 AIMP2 7965
Proximity Label-MS Homo sapiens
170 FAM50A 9130
Proximity Label-MS Homo sapiens
171 LIMD1 8994
Proximity Label-MS Homo sapiens
172 POLA1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
173 TMED7 51014
Proximity Label-MS Homo sapiens
174 PPAN 56342
Proximity Label-MS Homo sapiens
175 PRAF2 11230
Proximity Label-MS Homo sapiens
176 PPP2R2A 5520
Proximity Label-MS Homo sapiens
177 GABPA 2551
Proximity Label-MS Homo sapiens
178 DARS 1615
Proximity Label-MS Homo sapiens
179 SCAMP3 10067
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
180 BRI3BP 140707
Proximity Label-MS Homo sapiens
181 EXOSC8  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
182 CTNND1 1500
Proximity Label-MS Homo sapiens
183 PSMD3 5709
Proximity Label-MS Homo sapiens
184 MORC2  
Proximity Label-MS Homo sapiens
185 MCM3 4172
Proximity Label-MS Homo sapiens
186 KIF23 9493
Proximity Label-MS Homo sapiens
187 ACAP2 23527
Proximity Label-MS Homo sapiens
188 AAK1 22848
Proximity Label-MS Homo sapiens
189 TFB2M  
Proximity Label-MS Homo sapiens
190 CTBP2 1488
Proximity Label-MS Homo sapiens
191 DNTTIP1 116092
Affinity Capture-MS Homo sapiens
192 BTF3 689
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
193 ARFGAP2 84364
Proximity Label-MS Homo sapiens
194 NACA 4666
Proximity Label-MS Homo sapiens
195 THRAP3 9967
Proximity Label-MS Homo sapiens
196 USP39 10713
Proximity Label-MS Homo sapiens
197 NDUFB5 4711
Proximity Label-MS Homo sapiens
198 BIVM-ERCC5  
Proximity Label-MS Homo sapiens
199 SURF4 6836
Proximity Label-MS Homo sapiens
200 TRIM33 51592
Proximity Label-MS Homo sapiens
201 FAM21A 387680
Proximity Label-MS Homo sapiens
202 HSP90AB1 3326
Proximity Label-MS Homo sapiens
203 URB2  
Proximity Label-MS Homo sapiens
204 CAND1 55832
Proximity Label-MS Homo sapiens
205 MVK 4598
Proximity Label-MS Homo sapiens
206 DIEXF  
Proximity Label-MS Homo sapiens
207 GK  
Proximity Label-MS Homo sapiens
208 ARL8B 55207
Proximity Label-MS Homo sapiens
209 NDUFS2 4720
Proximity Label-MS Homo sapiens
210 RPS3 6188
Proximity Label-MS Homo sapiens
211 SAFB 6294
Proximity Label-MS Homo sapiens
212 TNRC6C  
Proximity Label-MS Homo sapiens
213 USO1 8615
Proximity Label-MS Homo sapiens
214 PSMA3 5684
Proximity Label-MS Homo sapiens
215 DPYSL2 1808
Proximity Label-MS Homo sapiens
216 DDX24 57062
Proximity Label-MS Homo sapiens
217 H1FX 8971
Proximity Label-MS Homo sapiens
218 COPS6 10980
Proximity Label-MS Homo sapiens
219 DSCR3 10311