Gene description for RPA2
Gene name replication protein A2, 32kDa
Gene symbol RPA2
Other names/aliases REPA2
RP-A p32
RP-A p34
RPA32
Species Homo sapiens
 Database cross references - RPA2
ExoCarta ExoCarta_6118
Entrez Gene 6118
HGNC 10290
MIM 179836
UniProt P15927  
 RPA2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for RPA2
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IPI
    protein N-terminus binding GO:0047485 IEA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    damaged DNA binding GO:0003684 IDA
    protein phosphatase binding GO:0019903 IPI
    single-stranded DNA binding GO:0003697 ISS
Biological Process
    DNA damage response, detection of DNA damage GO:0042769 TAS
    nucleotide-excision repair, DNA damage removal GO:0000718 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    regulation of DNA damage checkpoint GO:2000001 IMP
    regulation of cellular response to heat GO:1900034 TAS
    mismatch repair GO:0006298 TAS
    error-free translesion synthesis GO:0070987 TAS
    translesion synthesis GO:0019985 TAS
    base-excision repair GO:0006284 TAS
    mitotic cell cycle GO:0000278 TAS
    double-strand break repair GO:0006302 TAS
    DNA recombinase assembly GO:0000730 TAS
    mitotic G1 DNA damage checkpoint GO:0031571 IMP
    telomere maintenance GO:0000723 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    double-strand break repair via homologous recombination GO:0000724 TAS
    regulation of double-strand break repair via homologous recombination GO:0010569 IMP
    telomere maintenance via recombination GO:0000722 TAS
    DNA replication GO:0006260 TAS
    error-prone translesion synthesis GO:0042276 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    DNA repair GO:0006281 TAS
    nucleotide-excision repair GO:0006289 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    cellular response to heat GO:0034605 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    PML body GO:0016605 IDA
    DNA replication factor A complex GO:0005662 IPI
    nucleoplasm GO:0005654 TAS
    site of double-strand break GO:0035861 IDA
    chromosome, telomeric region GO:0000781 IDA
 Experiment description of studies that identified RPA2 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TP53BP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 TUBB2A 7280
Two-hybrid Homo sapiens
3 MCM3 4172
Two-hybrid Homo sapiens
4 ORC1L  
Two-hybrid Homo sapiens
5 TLE1  
Two-hybrid Homo sapiens
6 ORC2L  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
7 RIF1  
Two-hybrid Homo sapiens
8 DKFZP564O0523  
Two-hybrid Homo sapiens
9 KIN  
Co-fractionation Homo sapiens
10 COPS6 10980
Two-hybrid Homo sapiens
11 UNG2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 RPA1 6117
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
13 MEN1 4221
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
14 RAD52  
Affinity Capture-Western Homo sapiens
15 UTP14A 10813
Two-hybrid Homo sapiens
16 XPA  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 UNG  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 ORC5L  
Two-hybrid Homo sapiens
19 UNC119  
Two-hybrid Homo sapiens
20 MCM4 4173
Two-hybrid Homo sapiens
21 KIAA1377  
Two-hybrid Homo sapiens
22 PRKDC 5591
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 RPA3  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
24 MCM5 4174
Two-hybrid Homo sapiens
25 GAPDH 2597
Two-hybrid Homo sapiens
26 ACP5  
Two-hybrid Homo sapiens
27 MCM2 4171
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
28 ORC4L  
Two-hybrid Homo sapiens
29 XRCC6 2547
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 RPA4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
31 RPLP1 6176
Two-hybrid Homo sapiens
32 STAT3 6774
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 GOLM1 51280
Two-hybrid Homo sapiens
34 MED31  
Two-hybrid Homo sapiens
35 SERTAD3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
36 HERPUD1  
Two-hybrid Homo sapiens
37 CRMP1 1400
Two-hybrid Homo sapiens
38 EEF1A1 1915
Two-hybrid Homo sapiens
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