Experiment description of studies that identified COPS6 in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for COPS6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PBX2   Affinity Capture-MS Homo sapiens 2 COPS8 10920 Co-purification Homo sapiens 3 CDH10   Two-hybrid Homo sapiens 4 ROGDI 79641 Two-hybrid Homo sapiens 5 DLEU1   Two-hybrid Homo sapiens 6 TRDMT1   Two-hybrid Homo sapiens 7 PRKRA 8575 Two-hybrid Homo sapiens 8 COX17   Two-hybrid Homo sapiens 9 ZNF24   Two-hybrid Homo sapiens 10 EMD   Two-hybrid Homo sapiens 11 PSAP 5660 Two-hybrid Homo sapiens 12 SMN1 6606 Two-hybrid Homo sapiens 13 LAMA4 3910 Two-hybrid Homo sapiens 14 SULT1E1   Two-hybrid Homo sapiens 15 TK1 7083 Two-hybrid Homo sapiens 16 COX5A   Two-hybrid Homo sapiens 17 PAEP   Two-hybrid Homo sapiens 18 CRELD1   Two-hybrid Homo sapiens 19 C7orf19   Two-hybrid Homo sapiens 20 ERH 2079 Two-hybrid Homo sapiens 21 ZFHX1B   Two-hybrid Homo sapiens 22 PSMD11 5717 Two-hybrid Homo sapiens 23 EDN1   Two-hybrid Homo sapiens 24 HMOX2 3163 Two-hybrid Homo sapiens 25 HSU79303   Two-hybrid Homo sapiens 26 SLC2A1 6513 Two-hybrid Homo sapiens 27 RPL15 6138 Two-hybrid Homo sapiens 28 SAT   Two-hybrid Homo sapiens 29 S100A10 6281 Two-hybrid Homo sapiens 30 RFC5 5985 Two-hybrid Homo sapiens 31 SERPINB9 5272 Two-hybrid Homo sapiens 32 FAU   Two-hybrid Homo sapiens 33 PMF1   Two-hybrid Homo sapiens 34 DIS3L2 129563 Two-hybrid Homo sapiens 35 FLJ10350   Two-hybrid Homo sapiens 36 BTBD2   Two-hybrid Homo sapiens 37 PHYHIP 9796 Two-hybrid Homo sapiens 38 PFKL 5211 Two-hybrid Homo sapiens 39 C1orf174   Two-hybrid Homo sapiens 40 MNAT1   Two-hybrid Homo sapiens 41 RPA2 6118 Two-hybrid Homo sapiens 42 STK40   Two-hybrid Homo sapiens 43 PAFAH1B3 5050 Two-hybrid Homo sapiens 44 PTEN   Two-hybrid Homo sapiens 45 SNRPG 6637 Two-hybrid Homo sapiens 46 TRIB3   Two-hybrid Homo sapiens 47 PDZK1IP1 10158 Two-hybrid Homo sapiens 48 STX5A   Two-hybrid Homo sapiens 49 QTRT1 81890 Two-hybrid Homo sapiens 50 TDGF1   Two-hybrid Homo sapiens 51 SERPINA5 5104 Two-hybrid Homo sapiens 52 C4orf17   Two-hybrid Homo sapiens 53 LPL 4023 Two-hybrid Homo sapiens 54 MYCBP   Two-hybrid Homo sapiens 55 EIF3E 3646 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 56 WIPI-2   Two-hybrid Homo sapiens 57 RAB27A 5873 Two-hybrid Homo sapiens 58 CDKN2C   Two-hybrid Homo sapiens 59 EP300 2033 Two-hybrid Homo sapiens View the network

Pathways in which COPS6 is involved

No pathways found