Gene description for DMBT1
Gene name deleted in malignant brain tumors 1
Gene symbol DMBT1
Other names/aliases GP340
muclin
Species Homo sapiens
 Database cross references - DMBT1
ExoCarta ExoCarta_1755
Vesiclepedia VP_1755
Entrez Gene 1755
HGNC 2926
MIM 601969
UniProt Q9UGM3  
 DMBT1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 19837982    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells Unpublished / Not applicable
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
 Gene ontology annotations for DMBT1
Molecular Function
    lipopolysaccharide binding GO:0001530 IDA
    DNA binding GO:0003677 IDA
    scavenger receptor activity GO:0005044 IDA
    scavenger receptor activity GO:0005044 NAS
    scavenger receptor activity GO:0005044 TAS
    protein binding GO:0005515 IPI
    zymogen binding GO:0035375 ISS
    pattern recognition receptor activity GO:0038187 IDA
    pattern recognition receptor activity GO:0038187 TAS
    calcium-dependent protein binding GO:0048306 TAS
    lipoteichoic acid binding GO:0070891 IDA
    heparan sulfate binding GO:1904399 IDA
Biological Process
    receptor-mediated endocytosis GO:0006898 IDA
    defense response GO:0006952 IBA
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IEA
    epithelial cell differentiation GO:0030855 NAS
    epithelial cell differentiation GO:0030855 TAS
    induction of bacterial agglutination GO:0043152 IDA
    induction of bacterial agglutination GO:0043152 IMP
    induction of bacterial agglutination GO:0043152 TAS
    innate immune response GO:0045087 TAS
    defense response to Gram-negative bacterium GO:0050829 IMP
    defense response to Gram-negative bacterium GO:0050829 ISS
    defense response to Gram-positive bacterium GO:0050830 IDA
    defense response to Gram-positive bacterium GO:0050830 IMP
    defense response to Gram-positive bacterium GO:0050830 ISS
    defense response to virus GO:0051607 IEA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IDA
    phagocytic vesicle membrane GO:0030670 IDA
    extracellular matrix GO:0031012 IBA
    extracellular matrix GO:0031012 ISS
    zymogen granule membrane GO:0042589 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified DMBT1 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
9
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
10
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for DMBT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PMEPA1 56937
Affinity Capture-MS Homo sapiens
2 GNG8  
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FAM122C  
Affinity Capture-MS Homo sapiens
5 ICE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GSK3B 2932
Affinity Capture-MS Homo sapiens
7 TMEM176B 28959
Affinity Capture-MS Homo sapiens
8 SFTPD  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 USB1  
Affinity Capture-MS Homo sapiens
10 MAGEB4  
Affinity Capture-MS Homo sapiens
11 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ARHGAP12  
Affinity Capture-MS Homo sapiens
13 USP36  
Affinity Capture-MS Homo sapiens
14 TIMM10  
Affinity Capture-MS Homo sapiens
15 PCK2 5106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 EBF2  
Affinity Capture-MS Homo sapiens
17 CDK6 1021
Affinity Capture-MS Homo sapiens
18 SPNS3  
Affinity Capture-MS Homo sapiens
19 FNDC5  
Affinity Capture-MS Homo sapiens
20 FKBP14  
Affinity Capture-MS Homo sapiens
21 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
22 APEX1 328
Affinity Capture-RNA Homo sapiens
23 PPCS 79717
Affinity Capture-MS Homo sapiens
24 RIBC1  
Affinity Capture-MS Homo sapiens
25 FRMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 KLK10 5655
Affinity Capture-MS Homo sapiens
27 PRDM16 63976
Affinity Capture-MS Homo sapiens
28 TMEM257  
Affinity Capture-MS Homo sapiens
29 PARD6B 84612
Affinity Capture-MS Homo sapiens
30 SFTPA1  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
31 ITLN2  
Affinity Capture-MS Homo sapiens
32 COPS6 10980
Affinity Capture-MS Homo sapiens
33 HBM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 METTL3  
Affinity Capture-MS Homo sapiens
35 STK11 6794
Affinity Capture-MS Homo sapiens
36 PRKX 5613
Affinity Capture-MS Homo sapiens
37 EID1  
Affinity Capture-MS Homo sapiens
38 TWF1 5756
Affinity Capture-MS Homo sapiens
39 SFXN4  
Affinity Capture-MS Homo sapiens
40 CLCN2  
Affinity Capture-MS Homo sapiens
41 GJB5  
Affinity Capture-MS Homo sapiens
42 TCEB3B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DMBT1 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Surfactant metabolism TAS Reactome





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