Gene description for GSK3B
Gene name glycogen synthase kinase 3 beta
Gene symbol GSK3B
Other names/aliases -
Species Homo sapiens
 Database cross references - GSK3B
ExoCarta ExoCarta_2932
Entrez Gene 2932
HGNC 4617
MIM 605004
UniProt P49841  
 GSK3B identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for GSK3B
Molecular Function
    integrin binding GO:0005178 IEA
    tau protein binding GO:0048156 IEA
    NF-kappaB binding GO:0051059 IPI
    ionotropic glutamate receptor binding GO:0035255 IEA
    p53 binding GO:0002039 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    RNA polymerase II transcription factor binding GO:0001085 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    tau-protein kinase activity GO:0050321 IDA
    kinase activity GO:0016301 TAS
    beta-catenin binding GO:0008013 IPI
    ATP binding GO:0005524 IEA
    protein kinase A catalytic subunit binding GO:0034236 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
Biological Process
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IEA
    negative regulation of glycogen (starch) synthase activity GO:2000466 TAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    cell migration GO:0016477 IEA
    response to lithium ion GO:0010226 IEA
    negative regulation of protein binding GO:0032091 IDA
    hypermethylation of CpG island GO:0044027 IEA
    fat cell differentiation GO:0045444 IEA
    negative regulation of protein complex assembly GO:0031333 IMP
    organ morphogenesis GO:0009887 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    positive regulation of protein catabolic process GO:0045732 IC
    axon guidance GO:0007411 TAS
    protein phosphorylation GO:0006468 IDA
    cellular response to mechanical stimulus GO:0071260 IEA
    innate immune response GO:0045087 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    glycogen metabolic process GO:0005977 IDA
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 ISS
    regulation of microtubule-based process GO:0032886 IMP
    canonical Wnt signaling pathway GO:0060070 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    canonical Wnt signaling pathway involved in positive regulation of apoptotic process GO:0044337 IEA
    establishment of cell polarity GO:0030010 IEA
    circadian rhythm GO:0007623 ISS
    epithelial to mesenchymal transition GO:0001837 IMP
    protein autophosphorylation GO:0046777 IDA
    positive regulation of protein complex assembly GO:0031334 IDA
    negative regulation of dendrite morphogenesis GO:0050774 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 ISS
    re-entry into mitotic cell cycle GO:0000320 IEA
    negative regulation of neuron maturation GO:0014043 IEA
    positive regulation of axon extension GO:0045773 IEA
    regulation of cellular response to heat GO:1900034 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    positive regulation of cell-matrix adhesion GO:0001954 IMP
    cellular response to heat GO:0034605 TAS
    response to drug GO:0042493 IEA
    regulation of gene expression by genetic imprinting GO:0006349 IEA
    ER overload response GO:0006983 IDA
    negative regulation of glycogen biosynthetic process GO:0045719 TAS
    positive regulation of protein binding GO:0032092 ISS
    protein export from nucleus GO:0006611 IEA
    positive regulation of stem cell differentiation GO:2000738 IEA
    myoblast fusion GO:0007520 IEA
    hippocampus development GO:0021766 IMP
    superior temporal gyrus development GO:0071109 IMP
    negative regulation of type B pancreatic cell development GO:2000077 TAS
    negative regulation of NFAT protein import into nucleus GO:0051534 IMP
    intracellular signal transduction GO:0035556 IDA
    negative regulation of cardiac muscle hypertrophy GO:0010614 IEA
    positive regulation of GTPase activity GO:0043547 IMP
    positive regulation of protein export from nucleus GO:0046827 IDA
    negative regulation of MAP kinase activity GO:0043407 IEA
    protein localization to microtubule GO:0035372 IEA
    cellular response to interleukin-3 GO:0036016 ISS
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IEA
    regulation of neuronal synaptic plasticity GO:0048168 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    plasma membrane GO:0005886 IDA
    beta-catenin destruction complex GO:0030877 TAS
    cytoplasm GO:0005737 IDA
    growth cone GO:0030426 IEA
    ribonucleoprotein complex GO:0030529 IEA
    neuronal cell body GO:0043025 IEA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    membrane raft GO:0045121 IEA
    dendritic shaft GO:0043198 IEA
    centrosome GO:0005813 IDA
    dendritic spine GO:0043197 IEA
 Experiment description of studies that identified GSK3B in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GSK3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMYD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TSC1  
Affinity Capture-Western Homo sapiens
3 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
4 SNAI1  
Affinity Capture-MS Homo sapiens
5 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DHX36 170506
Affinity Capture-MS Homo sapiens
7 NOTCH2 4853
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
8 SGKL  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 TSC2 7249
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
10 AXIN1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 AKAP11 11215
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 CTNNB1 1499
Affinity Capture-Western Homo sapiens
13 PPP1CA 5499
Affinity Capture-Western Homo sapiens
14 PRKAR2A 5576
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 APC  
Affinity Capture-Western Homo sapiens
16 C14orf129  
Affinity Capture-MS Homo sapiens
17 CCNE1  
Biochemical Activity Homo sapiens
18 DDX20  
Affinity Capture-MS Homo sapiens
19 SMN2 6607
Affinity Capture-MS Homo sapiens
20 PTK2 5747
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
21 PTPN1 5770
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
22 UPF3A  
Two-hybrid Homo sapiens
23 AXIN2 8313
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
24 XPO6 23214
Affinity Capture-MS Homo sapiens
25 STRAP 11171
Affinity Capture-MS Homo sapiens
26 GEMIN4 50628
Affinity Capture-MS Homo sapiens
27 DNM1L 10059
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 SNRNP70 6625
Affinity Capture-MS Homo sapiens
29 NIN 51199
Two-hybrid Homo sapiens
30 PRKCA 5578
Biochemical Activity Homo sapiens
31 TP53  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
32 SF3B1 23451
Affinity Capture-MS Homo sapiens
33 AKT2 208
Co-crystal Structure Homo sapiens
34 NFKB1 4790
Biochemical Activity Homo sapiens
35 MUC1 4582
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 ILK 3611
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
37 EWSR1 2130
Affinity Capture-MS Homo sapiens
38 PRKCB 5579
Affinity Capture-MS Homo sapiens
39 AKT1 207
Reconstituted Complex Homo sapiens
40 ACLY 47
Affinity Capture-MS Homo sapiens
41 NOTCH1 4851
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
42 BXDC1  
Affinity Capture-MS Homo sapiens
43 AR 367
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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