Gene description for ATPIF1
Gene name ATPase inhibitory factor 1
Gene symbol ATPIF1
Other names/aliases ATPI
ATPIP
IP
Species Homo sapiens
 Database cross references - ATPIF1
ExoCarta ExoCarta_93974
Vesiclepedia VP_93974
Entrez Gene 93974
HGNC 871
MIM 614981
UniProt Q9UII2  
 ATPIF1 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for ATPIF1
Molecular Function
    enzyme inhibitor activity GO:0004857 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 ISS
    enzyme binding GO:0019899 IPI
    ATPase inhibitor activity GO:0042030 IBA
    ATPase inhibitor activity GO:0042030 IDA
    ATPase inhibitor activity GO:0042030 IMP
    ATPase inhibitor activity GO:0042030 ISS
    identical protein binding GO:0042802 ISS
    angiostatin binding GO:0043532 IDA
    ATPase binding GO:0051117 IBA
    ATPase binding GO:0051117 IDA
    ATPase binding GO:0051117 ISS
    mitochondrial proton-transporting ATP synthase complex binding GO:0140260 IDA
Biological Process
    angiogenesis GO:0001525 TAS
    negative regulation of endothelial cell proliferation GO:0001937 IDA
    generation of precursor metabolites and energy GO:0006091 TAS
    heme biosynthetic process GO:0006783 IBA
    erythrocyte differentiation GO:0030218 IBA
    negative regulation of ATP-dependent activity GO:0032780 IMP
    negative regulation of hydrolase activity GO:0051346 IDA
    mitochondrial depolarization GO:0051882 IMP
    reactive oxygen species metabolic process GO:0072593 IEA
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 IEA
    positive regulation of proteolysis involved in protein catabolic process GO:1903052 IMP
    regulation of protein targeting to mitochondrion GO:1903214 IMP
    regulation of ATP metabolic process GO:1903578 IMP
    positive regulation of type 2 mitophagy GO:1905091 IMP
    negative regulation of mitochondrial ATP synthesis coupled proton transport GO:1905707 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    cell surface GO:0009986 IDA
    protein-containing complex GO:0032991 ISS
 Experiment description of studies that identified ATPIF1 in exosomes
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATPIF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
3 UQCRFS1 7386
Co-fractionation Homo sapiens
4 PTMS 5763
Affinity Capture-MS Homo sapiens
5 MARCKS 4082
Affinity Capture-MS Homo sapiens
6 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
7 LHFPL4  
Affinity Capture-MS Homo sapiens
8 EPB41L5 57669
Affinity Capture-MS Homo sapiens
9 FBXL19  
Affinity Capture-MS Homo sapiens
10 MTG2  
Proximity Label-MS Homo sapiens
11 CALM1 801
Affinity Capture-MS Homo sapiens
12 PEBP1 5037
Affinity Capture-MS Homo sapiens
13 SYDE2  
Affinity Capture-MS Homo sapiens
14 PARK7 11315
Affinity Capture-MS Homo sapiens
15 MTMR9 66036
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LRPPRC 10128
Proximity Label-MS Homo sapiens
17 SGCZ  
Affinity Capture-MS Homo sapiens
18 TAX1BP3 30851
Co-fractionation Homo sapiens
19 PGK1 5230
Affinity Capture-MS Homo sapiens
20 CRYZ 1429
Proximity Label-MS Homo sapiens
21 PMPCA 23203
Proximity Label-MS Homo sapiens
22 ANP32B 10541
Affinity Capture-MS Homo sapiens
23 CLIP1 6249
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
24 SLC25A12 8604
Proximity Label-MS Homo sapiens
25 TIAM1  
Affinity Capture-MS Homo sapiens
26 MRPS26 64949
Proximity Label-MS Homo sapiens
27 ATP8  
Affinity Capture-MS Homo sapiens
28 ATP5O 539
Affinity Capture-MS Homo sapiens
29 ARHGAP19  
Affinity Capture-MS Homo sapiens
30 PDIA6 10130
Affinity Capture-MS Homo sapiens
31 CCDC18  
Cross-Linking-MS (XL-MS) Homo sapiens
32 RRP8  
Affinity Capture-MS Homo sapiens
33 RNASET2  
Co-fractionation Homo sapiens
34 ATP5H 10476
Affinity Capture-MS Homo sapiens
35 CALR 811
Affinity Capture-MS Homo sapiens
36 THAP8  
Affinity Capture-MS Homo sapiens
37 SPAG17  
Cross-Linking-MS (XL-MS) Homo sapiens
38 TACO1  
Affinity Capture-MS Homo sapiens
39 CHD3 1107
Two-hybrid Homo sapiens
40 UBQLN4 56893
Two-hybrid Homo sapiens
41 NEDD4 4734
Affinity Capture-MS Homo sapiens
42 SOD1 6647
Affinity Capture-MS Homo sapiens
43 BFAR  
Affinity Capture-MS Homo sapiens
44 GSK3B 2932
Two-hybrid Homo sapiens
45 MDH2 4191
Proximity Label-MS Homo sapiens
46 TUFM 7284
Proximity Label-MS Homo sapiens
47 UNK  
Affinity Capture-RNA Homo sapiens
48 PFN1 5216
Affinity Capture-MS Homo sapiens
49 HN1L 90861
Cross-Linking-MS (XL-MS) Homo sapiens
50 PPIB 5479
Affinity Capture-MS Homo sapiens
51 PGAM1 5223
Affinity Capture-MS Homo sapiens
52 C6orf203  
Proximity Label-MS Homo sapiens
53 ANTXR1 84168
Affinity Capture-MS Homo sapiens
54 NBR1 4077
Affinity Capture-MS Homo sapiens
55 GPR35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CERS2 29956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ATP6V0C 527
Affinity Capture-MS Homo sapiens
58 Atp5a1 11946
Affinity Capture-MS Mus musculus
59 YAP1 10413
Affinity Capture-MS Homo sapiens
60 HMGN1  
Affinity Capture-MS Homo sapiens
61 SUMO2 6613
Affinity Capture-MS Homo sapiens
62 API5 8539
Cross-Linking-MS (XL-MS) Homo sapiens
63 NELL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CSNK1E 1454
Affinity Capture-MS Homo sapiens
65 B4GALT3 8703
Affinity Capture-MS Homo sapiens
66 MTRF1L  
Proximity Label-MS Homo sapiens
67 TMEM70  
Proximity Label-MS Homo sapiens
68 ATP5B 506
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
69 GPS2  
Affinity Capture-MS Homo sapiens
70 RELL1 768211
Affinity Capture-MS Homo sapiens
71 DST 667
Cross-Linking-MS (XL-MS) Homo sapiens
72 CAT 847
Co-fractionation Homo sapiens
73 WBSCR16  
Proximity Label-MS Homo sapiens
74 MRRF  
Proximity Label-MS Homo sapiens
75 MRPS12  
Proximity Label-MS Homo sapiens
76 ATP5I 521
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 GUCY2C  
Affinity Capture-MS Homo sapiens
78 MTIF3  
Proximity Label-MS Homo sapiens
79 ATP6  
Affinity Capture-MS Homo sapiens
80 SURF6  
Affinity Capture-MS Homo sapiens
81 P2RY2 5029
Affinity Capture-MS Homo sapiens
82 ATP5F1 515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 PDIA3 2923
Affinity Capture-MS Homo sapiens
84 GAA 2548
Co-fractionation Homo sapiens
85 ABCB1 5243
Cross-Linking-MS (XL-MS) Homo sapiens
86 CORO1C 23603
Cross-Linking-MS (XL-MS) Homo sapiens
87 BASP1 10409
Affinity Capture-MS Homo sapiens
88 BLK 640
Affinity Capture-MS Homo sapiens
89 CD27  
Affinity Capture-MS Homo sapiens
90 ERICH6B  
Cross-Linking-MS (XL-MS) Homo sapiens
91 CEP250 11190
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
92 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
93 ARHGAP29 9411
Affinity Capture-MS Homo sapiens
94 ATP5E 514
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
95 PHF6  
Affinity Capture-MS Homo sapiens
96 ZDHHC22  
Affinity Capture-MS Homo sapiens
97 ACKR2  
Affinity Capture-MS Homo sapiens
98 PIK3CA 5290
Two-hybrid Homo sapiens
99 Abr 109934
Affinity Capture-MS Mus musculus
100 HSPA4 3308
Cross-Linking-MS (XL-MS) Homo sapiens
101 ACAD9 28976
Proximity Label-MS Homo sapiens
102 STMN1 3925
Affinity Capture-MS Homo sapiens
103 TMEM101  
Affinity Capture-MS Homo sapiens
104 VIPR1  
Affinity Capture-MS Homo sapiens
105 MSN 4478
Cross-Linking-MS (XL-MS) Homo sapiens
106 METTL15  
Proximity Label-MS Homo sapiens
107 UBXN1 51035
Cross-Linking-MS (XL-MS) Homo sapiens
108 FAM133A 286499
Affinity Capture-MS Homo sapiens
109 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
110 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
111 FBXL6  
Proximity Label-MS Homo sapiens
112 PRKCSH 5589
Affinity Capture-MS Homo sapiens
113 TSFM 10102
Proximity Label-MS Homo sapiens
114 UBXN4 23190
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
115 ATP5J 522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 HMGB1 3146
Affinity Capture-MS Homo sapiens
117 ZFYVE27 118813
Affinity Capture-MS Homo sapiens
118 PDIA4 9601
Affinity Capture-MS Homo sapiens
119 ASPSCR1 79058
Cross-Linking-MS (XL-MS) Homo sapiens
120 ATP5G3 518
Affinity Capture-MS Homo sapiens
121 RPS21 6227
Affinity Capture-MS Homo sapiens
122 PDHA1 5160
Proximity Label-MS Homo sapiens
123 CYTH2 9266
Affinity Capture-MS Homo sapiens
124 COX8A  
Proximity Label-MS Homo sapiens
125 TKT 7086
Affinity Capture-MS Homo sapiens
126 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
127 C21orf33  
Proximity Label-MS Homo sapiens
128 MIF 4282
Affinity Capture-MS Homo sapiens
129 C10orf88  
Affinity Capture-MS Homo sapiens
130 TBRG4 9238
Proximity Label-MS Homo sapiens
131 AARS2  
Proximity Label-MS Homo sapiens
132 ARL3 403
Co-fractionation Homo sapiens
133 CEP135  
Cross-Linking-MS (XL-MS) Homo sapiens
134 RDX 5962
Cross-Linking-MS (XL-MS) Homo sapiens
135 ANXA5 308
Affinity Capture-MS Homo sapiens
136 C1QBP 708
Proximity Label-MS Homo sapiens
137 EI24  
Affinity Capture-MS Homo sapiens
138 PRPF4 9128
Cross-Linking-MS (XL-MS) Homo sapiens
139 ATP5C1 509
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 METTL17  
Proximity Label-MS Homo sapiens
141 DDX28  
Proximity Label-MS Homo sapiens
142 ATP5A1 498
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATPIF1 is involved
No pathways found





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