Gene description for ATP6V0C
Gene name ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c
Gene symbol ATP6V0C
Other names/aliases ATP6C
ATP6L
ATPL
VATL
VPPC
Vma3
Species Homo sapiens
 Database cross references - ATP6V0C
ExoCarta ExoCarta_527
Vesiclepedia VP_527
Entrez Gene 527
HGNC 855
MIM 108745
UniProt P27449  
 ATP6V0C identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for ATP6V0C
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 TAS
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
Biological Process
    vacuolar acidification GO:0007035 NAS
    lysosomal lumen acidification GO:0007042 NAS
    proton motive force-driven ATP synthesis GO:0015986 IEA
    regulation of macroautophagy GO:0016241 NAS
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    endosomal lumen acidification GO:0048388 NAS
    intracellular pH reduction GO:0051452 NAS
    Golgi lumen acidification GO:0061795 NAS
    synaptic vesicle lumen acidification GO:0097401 IEA
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 ISS
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    endosome membrane GO:0010008 NAS
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    phagocytic vesicle membrane GO:0030670 TAS
    synaptic vesicle membrane GO:0030672 IEA
    proton-transporting V-type ATPase complex GO:0033176 NAS
    azurophil granule membrane GO:0035577 TAS
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified ATP6V0C in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP6V0C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 HBB 3043
Affinity Capture-MS Homo sapiens
4 ATPIF1 93974
Affinity Capture-MS Homo sapiens
5 CMTM8  
Affinity Capture-MS Homo sapiens
6 KIAA2013 90231
Affinity Capture-MS Homo sapiens
7 ASGR2  
Two-hybrid Homo sapiens
8 BSCL2  
Affinity Capture-MS Homo sapiens
9 MDFI  
Two-hybrid Homo sapiens
10 LGALS1 3956
Affinity Capture-MS Homo sapiens
11 TMEM186  
Affinity Capture-MS Homo sapiens
12 PI16  
Two-hybrid Homo sapiens
13 CD79A  
Two-hybrid Homo sapiens
14 ATP5L 10632
Affinity Capture-MS Homo sapiens
15 MGST1 4257
Co-fractionation Homo sapiens
16 C10orf54 64115
Two-hybrid Homo sapiens
17 OPRM1 4988
Two-hybrid Homo sapiens
18 KLRC1  
Two-hybrid Homo sapiens
19 C10orf35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MTUS2 23281
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CLEC14A  
Two-hybrid Homo sapiens
22 ATP8  
Affinity Capture-MS Homo sapiens
23 ATP5O 539
Affinity Capture-MS Homo sapiens
24 ATP5C1 509
Affinity Capture-MS Homo sapiens
25 ERMP1 79956
Affinity Capture-MS Homo sapiens
26 ATP5D 513
Affinity Capture-MS Homo sapiens
27 KIF2C 11004
Affinity Capture-MS Homo sapiens
28 CCDC115 84317
Affinity Capture-MS Homo sapiens
29 FNDC1 84624
Affinity Capture-MS Homo sapiens
30 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ATP6V0A4 50617
Affinity Capture-MS Homo sapiens
32 ATP6AP1 537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 STX12 23673
Affinity Capture-MS Homo sapiens
34 TMEM87A 25963
Affinity Capture-MS Homo sapiens
35 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
36 MCEMP1  
Two-hybrid Homo sapiens
37 CAMK2A 815
Affinity Capture-MS Homo sapiens
38 NEDD8-MDP1 100528064
Affinity Capture-MS Homo sapiens
39 ATP5A1 498
Affinity Capture-MS Homo sapiens
40 GPR25  
Two-hybrid Homo sapiens
41 ATP6V1G2  
Affinity Capture-MS Homo sapiens
42 STOM 2040
Two-hybrid Homo sapiens
43 CERS3  
Affinity Capture-MS Homo sapiens
44 PDCD1LG2 80380
Two-hybrid Homo sapiens
45 CERS2 29956
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
46 KDSR 2531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PILRB 29990
Affinity Capture-MS Homo sapiens
48 CD53 963
Two-hybrid Homo sapiens
49 PTPRE 5791
Affinity Capture-MS Homo sapiens
50 CYB5A 1528
Co-fractionation Homo sapiens
51 TMEM259 91304
Affinity Capture-MS Homo sapiens
52 CREB3  
Two-hybrid Homo sapiens
53 SLC35E3  
Two-hybrid Homo sapiens
54 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
55 VMA21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TECR 9524
Affinity Capture-MS Homo sapiens
57 SCN3B  
Two-hybrid Homo sapiens
58 ATP5B 506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 YIF1B 90522
Affinity Capture-MS Homo sapiens
60 SLC7A1 6541
Two-hybrid Homo sapiens
61 TMEM185A  
Affinity Capture-MS Homo sapiens
62 PSMA3 5684
Two-hybrid Homo sapiens
63 TMEM199  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 HADHB 3032
Co-fractionation Homo sapiens
65 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CANX 821
Affinity Capture-MS Homo sapiens
67 FFAR2  
Two-hybrid Homo sapiens
68 LRRC25  
Two-hybrid Homo sapiens
69 ATP6  
Affinity Capture-MS Homo sapiens
70 VAMP3 9341
Affinity Capture-MS Homo sapiens
71 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ATP5F1 515
Affinity Capture-MS Homo sapiens
73 SLC34A2 10568
Affinity Capture-MS Homo sapiens
74 TMEM139  
Two-hybrid Homo sapiens
75 HSD17B13  
Two-hybrid Homo sapiens
76 SUSD3 203328
Two-hybrid Homo sapiens
77 TCIRG1 10312
Affinity Capture-MS Homo sapiens
78 CD27  
Affinity Capture-MS Homo sapiens
79 GPR42  
Two-hybrid Homo sapiens
80 FAM134C 162427
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
81 HTR2C  
Two-hybrid Homo sapiens
82 CPLX4  
Two-hybrid Homo sapiens
83 EVI2A  
Two-hybrid Homo sapiens
84 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 ZCCHC12  
Two-hybrid Homo sapiens
86 DRD2  
Two-hybrid Homo sapiens
87 SLC14A1  
Affinity Capture-MS Homo sapiens
88 GBAS 2631
Co-fractionation Homo sapiens
89 SSMEM1  
Two-hybrid Homo sapiens
90 MARK3 4140
Two-hybrid Homo sapiens
91 ARRDC5  
Affinity Capture-MS Homo sapiens
92 SPACA1 81833
Two-hybrid Homo sapiens
93 SMIM3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
94 CLEC4A  
Affinity Capture-MS Homo sapiens
95 CLIC1 1192
Two-hybrid Homo sapiens
96 TMEM9 252839
Two-hybrid Homo sapiens
97 CD69  
Two-hybrid Homo sapiens
98 ATP5J 522
Affinity Capture-MS Homo sapiens
99 ERH 2079
Two-hybrid Homo sapiens
100 SH2D3A  
Affinity Capture-MS Homo sapiens
101 GPR152  
Two-hybrid Homo sapiens
102 DDX58 23586
Affinity Capture-RNA Homo sapiens
103 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
104 ATP5G1  
Affinity Capture-MS Homo sapiens
105 XKR4  
Affinity Capture-MS Homo sapiens
106 EDA  
Two-hybrid Homo sapiens
107 TAPBP 6892
Affinity Capture-MS Homo sapiens
108 MSR1  
Two-hybrid Homo sapiens
109 RNF182  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
110 CYB5R3 1727
Affinity Capture-MS Homo sapiens
111 EDA2R  
Two-hybrid Homo sapiens
112 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 DNAJC1 64215
Two-hybrid Homo sapiens
114 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
115 TMEM31  
Two-hybrid Homo sapiens
116 C17orf59 54785
Affinity Capture-MS Homo sapiens
117 FAM210B  
Two-hybrid Homo sapiens
118 CLEC17A  
Two-hybrid Homo sapiens
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