Gene description for ATP6V1F
Gene name ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
Gene symbol ATP6V1F
Other names/aliases ATP6S14
VATF
Vma7
Species Homo sapiens
 Database cross references - ATP6V1F
ExoCarta ExoCarta_9296
Entrez Gene 9296
HGNC 16832
MIM 607160
UniProt Q16864  
 ATP6V1F identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for ATP6V1F
Molecular Function
    ATPase activity, coupled to transmembrane movement of ions GO:0042625 IBA
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    hydrogen ion transmembrane transporter activity GO:0015078 NAS
    ATPase activity, uncoupled GO:0042624 NAS
    protein binding GO:0005515 IPI
Biological Process
    ATP hydrolysis coupled proton transport GO:0015991 IEA
    interaction with host GO:0051701 TAS
    metabolic process GO:0008152 NAS
    ion transmembrane transport GO:0034220 TAS
    phagosome maturation GO:0090382 TAS
    cellular iron ion homeostasis GO:0006879 TAS
    transferrin transport GO:0033572 TAS
    proton transport GO:0015992 NAS
    insulin receptor signaling pathway GO:0008286 TAS
    transmembrane transport GO:0055085 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IDA
    proton-transporting V-type ATPase, V1 domain GO:0033180 IEA
    proton-transporting two-sector ATPase complex GO:0016469 NAS
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified ATP6V1F in exosomes
1
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP6V1F
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIAA1377  
Two-hybrid Homo sapiens
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