Gene description for Vps4b
Gene name vacuolar protein sorting 4b (yeast)
Gene symbol Vps4b
Other names/aliases 8030489C12Rik
Skd1
Species Mus musculus
 Database cross references - Vps4b
ExoCarta ExoCarta_20479
Vesiclepedia VP_20479
Entrez Gene 20479
UniProt P46467  
 Vps4b identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps4b
Molecular Function
    identical protein binding GO:0042802 ISO
    protein C-terminus binding GO:0008022 ISO
    ATPase activity GO:0016887 ISO
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
    protein homodimerization activity GO:0042803 ISO
    ATPase activity, coupled GO:0042623 ISO
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IEA
Biological Process
    regulation of mitotic spindle assembly GO:1901673 ISO
    protein transport GO:0015031 IEA
    protein depolymerization GO:0051261 ISO
    endosome to lysosome transport via multivesicular body sorting pathway GO:0032510 ISO
    regulation of viral process GO:0050792 ISO
    positive regulation of centriole elongation GO:1903724 ISO
    response to lipid GO:0033993 ISO
    cell cycle GO:0007049 IEA
    vacuole organization GO:0007033 IBA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    negative regulation of cell death GO:0060548 ISO
    potassium ion transport GO:0006813 IDA
    transport GO:0006810 IEA
    cell separation after cytokinesis GO:0000920 ISO
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    viral release from host cell GO:0019076 ISO
    endosomal transport GO:0016197 ISO
    cell division GO:0051301 IEA
    mitotic metaphase plate congression GO:0007080 ISO
    endosome organization GO:0007032 IMP
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    negative regulation of exosomal secretion GO:1903542 ISO
    nucleus organization GO:0006997 ISO
    positive regulation of viral release from host cell GO:1902188 ISO
    cholesterol transport GO:0030301 IMP
    positive regulation of exosomal secretion GO:1903543 ISO
    positive regulation of viral life cycle GO:1903902 ISO
    regulation of centrosome duplication GO:0010824 ISO
    positive regulation of viral process GO:0048524 ISO
Subcellular Localization
    endosome GO:0005768 ISO
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 IDA
    Flemming body GO:0090543 ISO
    endosome membrane GO:0010008 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    nucleus GO:0005634 ISO
    spindle pole GO:0000922 ISO
 Experiment description of studies that identified Vps4b in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vps4b is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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