Gene description for DDX19B
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B
Gene symbol DDX19B
Other names/aliases DBP5
DDX19
RNAh
Species Homo sapiens
 Database cross references - DDX19B
ExoCarta ExoCarta_11269
Entrez Gene 11269
HGNC 2742
MIM 605812
UniProt Q9UMR2  
 DDX19B identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for DDX19B
Molecular Function
    protein binding GO:0005515 IPI
    ATP-dependent RNA helicase activity GO:0004004 IBA
    ATP binding GO:0005524 IEA
    RNA binding GO:0003723 IEA
    helicase activity GO:0004386 TAS
Biological Process
    protein transport GO:0015031 IEA
    mRNA export from nucleus GO:0006406 TAS
    RNA secondary structure unwinding GO:0010501 IBA
Subcellular Localization
    nuclear pore GO:0005643 IEA
    extracellular exosome GO:0070062 IDA
    nuclear envelope GO:0005635 IDA
    nuclear membrane GO:0031965 IEA
    cytoplasm GO:0005737 IEA
    membrane GO:0016020 IDA
 Experiment description of studies that identified DDX19B in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DDX19B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUP214  
Invitro Homo sapiens
2 GNB2L1 10399
Two-hybrid Homo sapiens
3 DCK 1633
Two-hybrid Homo sapiens
4 XPO7 23039
Two-hybrid Homo sapiens
5 KLHDC2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DDX19B is involved
No pathways found





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