Gene description for SERPINB2
Gene name serpin peptidase inhibitor, clade B (ovalbumin), member 2
Gene symbol SERPINB2
Other names/aliases HsT1201
PAI
PAI-2
PAI2
PLANH2
Species Homo sapiens
 Database cross references - SERPINB2
ExoCarta ExoCarta_5055
Vesiclepedia VP_5055
Entrez Gene 5055
HGNC 8584
MIM 173390
UniProt P05120  
 SERPINB2 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for SERPINB2
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 TAS
Biological Process
    negative regulation of endopeptidase activity GO:0010951 IBA
    fibrinolysis GO:0042730 TAS
    negative regulation of apoptotic process GO:0043066 IEA
Subcellular Localization
    cornified envelope GO:0001533 IEA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified SERPINB2 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SERPINB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLC2 64837
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SEP15 9403
Affinity Capture-MS Homo sapiens
3 RBL2 5934
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 METTL7A 25840
Affinity Capture-MS Homo sapiens
5 FKBP15 23307
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CMPK1 51727
Co-fractionation Homo sapiens
7 UBE3A 7337
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
8 TAB1 10454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SOX2  
Affinity Capture-MS Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 PI4KA 5297
Affinity Capture-MS Homo sapiens
12 CHST10  
Affinity Capture-MS Homo sapiens
13 RALGAPB 57148
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GPATCH2L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PLAU 5328
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
17 IFT74 80173
Affinity Capture-MS Homo sapiens
18 CA8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 OSBP 5007
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 YES1 7525
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 IFT81 28981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 LPP 4026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GDF5 8200
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PHF8  
Affinity Capture-MS Homo sapiens
25 PHF11 51131
Affinity Capture-MS Homo sapiens
26 GOLGA4  
Affinity Capture-MS Homo sapiens
27 C18orf21  
Affinity Capture-MS Homo sapiens
28 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C21orf59 56683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RB1 5925
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 VAV1 7409
Affinity Capture-MS Homo sapiens
33 PRPF8 10594
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
34 SERPINB1 1992
Co-fractionation Homo sapiens
35 C17orf75 64149
Affinity Capture-MS Homo sapiens
36 ZDHHC19  
Affinity Capture-MS Homo sapiens
37 RNF115  
Affinity Capture-MS Homo sapiens
38 PSMB1 5689
Affinity Capture-Western Homo sapiens
39 TAF5L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SRRT 51593
Affinity Capture-MS Homo sapiens
41 CCDC51 79714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MMAB 326625
Affinity Capture-MS Homo sapiens
43 SARAF  
Affinity Capture-MS Homo sapiens
44 PIGT 51604
Affinity Capture-MS Homo sapiens
45 SLC25A32 81034
Affinity Capture-MS Homo sapiens
46 SSUH2  
Affinity Capture-MS Homo sapiens
47 AQP12B  
Affinity Capture-MS Homo sapiens
48 SCN3B  
Affinity Capture-MS Homo sapiens
49 DNM1L 10059
Co-fractionation Homo sapiens
50 CHMP3 51652
Affinity Capture-MS Homo sapiens
51 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PLAT 5327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
54 AGPAT1 10554
Affinity Capture-MS Homo sapiens
55 CCNYL1 151195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ZC3HC1  
Affinity Capture-MS Homo sapiens
57 NUP54 53371
Affinity Capture-MS Homo sapiens
58 PTK2 5747
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RBMS1 5937
Co-fractionation Homo sapiens
60 NUP214 8021
Affinity Capture-MS Homo sapiens
61 ADH1C 126
Affinity Capture-MS Homo sapiens
62 KDM3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PI4KAP1  
Affinity Capture-MS Homo sapiens
64 SIK1  
Affinity Capture-MS Homo sapiens
65 DIS3 22894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 TBL1X 6907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HDLBP 3069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SLC37A4 2542
Affinity Capture-MS Homo sapiens
69 PPFIA1 8500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 STX17 55014
Affinity Capture-MS Homo sapiens
71 DNAJC13 23317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 FNDC5  
Affinity Capture-MS Homo sapiens
73 TAF5  
Affinity Capture-MS Homo sapiens
74 SPICE1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 MAP2K2 5605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 KIAA0907  
Affinity Capture-MS Homo sapiens
77 SLC25A6 293
Affinity Capture-MS Homo sapiens
78 SYAP1 94056
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 RBM24 221662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 EEA1 8411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 POLR2L 5441
Affinity Capture-MS Homo sapiens
82 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MICU3  
Affinity Capture-MS Homo sapiens
84 RSPRY1 89970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 CCR1  
Affinity Capture-MS Homo sapiens
86 C1orf35  
Affinity Capture-MS Homo sapiens
87 SSBP2  
Affinity Capture-MS Homo sapiens
88 HNRNPA3 220988
Co-fractionation Homo sapiens
89 SELO 83642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 KIR3DS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ITPA 3704
Co-fractionation Homo sapiens
92 PINK1  
Affinity Capture-MS Homo sapiens
93 GOT1 2805
Affinity Capture-MS Homo sapiens
94 DDX42 11325
Affinity Capture-MS Homo sapiens
95 OR2A4 79541
Affinity Capture-MS Homo sapiens
96 ARHGAP35 2909
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 RAPGEF6 51735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 ERP27  
Affinity Capture-MS Homo sapiens
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