Gene description for RSPRY1
Gene name ring finger and SPRY domain containing 1
Gene symbol RSPRY1
Other names/aliases -
Species Homo sapiens
 Database cross references - RSPRY1
ExoCarta ExoCarta_89970
Vesiclepedia VP_89970
Entrez Gene 89970
HGNC 29420
UniProt Q96DX4  
 RSPRY1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RSPRY1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis involved in protein catabolic process GO:0051603 IBA
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified RSPRY1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RSPRY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GCNT3 9245
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NES 10763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SLC15A1  
Affinity Capture-MS Homo sapiens
6 EFNA4  
Affinity Capture-MS Homo sapiens
7 SPATA19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TMPRSS11E  
Affinity Capture-MS Homo sapiens
9 CLEC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CUL3 8452
Affinity Capture-MS Homo sapiens
11 NPHP3 27031
Affinity Capture-MS Homo sapiens
12 NODAL  
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
15 COX4I2 84701
Affinity Capture-MS Homo sapiens
16 MRPL17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PRIMPOL  
Affinity Capture-MS Homo sapiens
18 SLC22A4 6583
Affinity Capture-MS Homo sapiens
19 SPINK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 WNT7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 GFAP 2670
Affinity Capture-MS Homo sapiens
22 EMID1  
Affinity Capture-MS Homo sapiens
23 VDAC1 7416
Affinity Capture-MS Homo sapiens
24 KLRK1  
Affinity Capture-MS Homo sapiens
25 HTR3D  
Affinity Capture-MS Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 CPQ 10404
Affinity Capture-MS Homo sapiens
28 SLC5A6 8884
Affinity Capture-MS Homo sapiens
29 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CDK5 1020
Affinity Capture-MS Homo sapiens
31 CCNJL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 AGK 55750
Affinity Capture-MS Homo sapiens
33 NEFM 4741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ITM2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PDE9A  
Affinity Capture-MS Homo sapiens
36 ADAMTSL1 92949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 EDN3  
Affinity Capture-MS Homo sapiens
39 XAGE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CDK5R1  
Affinity Capture-MS Homo sapiens
41 ISM2  
Affinity Capture-MS Homo sapiens
42 UBE2D2 7322
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
43 PARK2  
Affinity Capture-MS Homo sapiens
44 IBSP  
Affinity Capture-MS Homo sapiens
45 PLD3 23646
Affinity Capture-MS Homo sapiens
46 INA 9118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C5orf30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 NEFL 4747
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 IL17B 27190
Affinity Capture-MS Homo sapiens
50 INF2 64423
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PCDHGA9  
Affinity Capture-MS Homo sapiens
52 UBE2D3 7323
Two-hybrid Homo sapiens
53 MALL  
Affinity Capture-MS Homo sapiens
54 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CFB 629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 FBXW7  
Synthetic Lethality Homo sapiens
57 UNC119 9094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SLC18A1  
Affinity Capture-MS Homo sapiens
59 NDUFA10 4705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PRND  
Affinity Capture-MS Homo sapiens
62 DDX58 23586
Affinity Capture-RNA Homo sapiens
63 VIM 7431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ITM2C 81618
Affinity Capture-MS Homo sapiens
66 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 GALNT3 2591
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CACNG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 DKK2  
Affinity Capture-MS Homo sapiens
70 TMPRSS11A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RSPRY1 is involved
No pathways found





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