Gene description for NEFM
Gene name neurofilament, medium polypeptide
Gene symbol NEFM
Other names/aliases NEF3
NF-M
NFM
Species Homo sapiens
 Database cross references - NEFM
ExoCarta ExoCarta_4741
Entrez Gene 4741
HGNC 7734
MIM 162250
UniProt P07197  
 NEFM identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
 Gene ontology annotations for NEFM
Molecular Function
    microtubule binding GO:0008017 TAS
    protein binding GO:0005515 IPI
    structural constituent of cytoskeleton GO:0005200 TAS
Biological Process
    regulation of axon diameter GO:0031133 IEA
    microtubule cytoskeleton organization GO:0000226 IEA
    neurofilament bundle assembly GO:0033693 TAS
    axon cargo transport GO:0008088 IEA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
Subcellular Localization
    neurofilament GO:0005883 IEA
    neurofibrillary tangle GO:0097418 IDA
    axon GO:0030424 TAS
    neuromuscular junction GO:0031594 IEA
 Experiment description of studies that identified NEFM in exosomes
1
Experiment ID 267
ISEV standards
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NEFM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NEFH 4744
Invitro Homo sapiens
2 APOE 348
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which NEFM is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here