Gene description for MCM7
Gene name minichromosome maintenance complex component 7
Gene symbol MCM7
Other names/aliases CDC47
MCM2
P1.1-MCM3
P1CDC47
P85MCM
PNAS146
PPP1R104
Species Homo sapiens
 Database cross references - MCM7
ExoCarta ExoCarta_4176
Entrez Gene 4176
HGNC 6950
MIM 600592
UniProt P33993  
 MCM7 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MCM7
Molecular Function
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    ATP-dependent DNA helicase activity GO:0004003 IDA
    single-stranded DNA binding GO:0003697 IEA
    DNA binding GO:0003677 TAS
Biological Process
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    DNA replication initiation GO:0006270 IEA
    DNA replication GO:0006260 TAS
    cell proliferation GO:0008283 IEA
    DNA unwinding involved in DNA replication GO:0006268 IEA
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    mitotic cell cycle GO:0000278 TAS
    regulation of phosphorylation GO:0042325 IMP
    response to drug GO:0042493 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
    cellular response to DNA damage stimulus GO:0006974 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    chromatin GO:0000785 TAS
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 IDA
    MCM complex GO:0042555 IMP
    cytosol GO:0005829 IEA
 Experiment description of studies that identified MCM7 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
4
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCM7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBL1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
2 ASK  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 CDK7  
Invivo Homo sapiens
5 MCM8 84515
Affinity Capture-Western Homo sapiens
6 ORC1L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 ATR  
Affinity Capture-Western Homo sapiens
8 GIPC1 10755
Affinity Capture-MS Homo sapiens
9 CCNH  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
10 ORC2L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 RBM8A 9939
Two-hybrid Homo sapiens
12 NEK6 10783
Affinity Capture-MS Homo sapiens
13 NELFCD 51497
Affinity Capture-MS Homo sapiens
14 MCM5 4174
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
15 MCM3 4172
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 RPA1 6117
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 TREX1  
Affinity Capture-MS Homo sapiens
18 TRAF6 7189
Affinity Capture-MS Homo sapiens
19 UBE3A 7337
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 CDC6  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 ORC5L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 PELO 53918
Affinity Capture-MS Homo sapiens
23 MCM4 4173
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
24 HIST3H3 8290
Invitro Homo sapiens
25 RB1 5925
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
26 MCM10 55388
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
27 MBIP  
Two-hybrid Homo sapiens
28 FHL2  
Two-hybrid Homo sapiens
29 MYC  
Affinity Capture-MS Homo sapiens
30 CNOT8  
Affinity Capture-MS Homo sapiens
31 CDC45L  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
32 MCM2 4171
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
33 RBL2 5934
Reconstituted Complex Homo sapiens
34 ORC4L  
Affinity Capture-MS Homo sapiens
35 GSTK1 373156
Affinity Capture-MS Homo sapiens
36 ATRIP  
Affinity Capture-Western Homo sapiens
37 MNAT1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 CDC7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 MCM6 4175
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
40 ORC3L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
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