Gene description for NAB2
Gene name NGFI-A binding protein 2 (EGR1 binding protein 2)
Gene symbol NAB2
Other names/aliases MADER
Species Homo sapiens
 Database cross references - NAB2
ExoCarta ExoCarta_4665
Vesiclepedia VP_4665
Entrez Gene 4665
HGNC 7627
MIM 602381
UniProt Q15742  
 NAB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NAB2
Molecular Function
    transcription coregulator activity GO:0003712 IBA
    transcription corepressor activity GO:0003714 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    endochondral ossification GO:0001958 IEA
    regulation of DNA-templated transcription GO:0006355 IBA
    Schwann cell differentiation GO:0014037 IEA
    negative regulation of transcription by RNA polymerase III GO:0016480 IDA
    myelination GO:0042552 IEA
    regulation of epidermis development GO:0045682 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified NAB2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NAB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCRS1  
Two-hybrid Homo sapiens
2 MCM7 4176
Two-hybrid Homo sapiens
3 ZMYND19  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 ENKD1  
Two-hybrid Homo sapiens
5 SENP3 26168
Affinity Capture-MS Homo sapiens
6 TNIP1 10318
Affinity Capture-MS Homo sapiens
7 NPRL2  
Affinity Capture-MS Homo sapiens
8 CUEDC2  
Affinity Capture-MS Homo sapiens
9 RBPMS 11030
Two-hybrid Homo sapiens
10 KIFC3 3801
Two-hybrid Homo sapiens
11 AES 166
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 ARL15 54622
Affinity Capture-MS Homo sapiens
13 MDFI  
Two-hybrid Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 ZMAT2  
Two-hybrid Homo sapiens
16 PLOD3 8985
Two-hybrid Homo sapiens
17 RINT1 60561
Two-hybrid Homo sapiens
18 EGR1  
Two-hybrid Homo sapiens
19 NME7 29922
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 TRIM35 23087
Affinity Capture-MS Homo sapiens
21 RFXANK  
Affinity Capture-MS Homo sapiens
22 TLN1 7094
Co-fractionation Homo sapiens
23 LENG1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 HES6  
Affinity Capture-MS Homo sapiens
25 KRT40  
Two-hybrid Homo sapiens
26 QARS 5859
Two-hybrid Homo sapiens
27 TCEA2  
Two-hybrid Homo sapiens
28 KRTAP10-3  
Two-hybrid Homo sapiens
29 DDX6 1656
Two-hybrid Homo sapiens
30 PHF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 NMT1 4836
Co-fractionation Homo sapiens
32 NPAS1  
Affinity Capture-MS Homo sapiens
33 CHD3 1107
Affinity Capture-Western Homo sapiens
34 YPEL3  
Affinity Capture-MS Homo sapiens
35 GOLGA6L9  
Two-hybrid Homo sapiens
36 NEDD9 4739
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 FAM96A  
Affinity Capture-MS Homo sapiens
38 KRT34  
Two-hybrid Homo sapiens
39 TSGA10IP  
Two-hybrid Homo sapiens
40 C1orf86  
Affinity Capture-MS Homo sapiens
41 MAPK3 5595
Two-hybrid Homo sapiens
42 PLBD1 79887
Affinity Capture-MS Homo sapiens
43 RFC5 5985
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 EGR2  
Biochemical Activity Homo sapiens
45 CCDC33 80125
Two-hybrid Homo sapiens
46 SCNM1  
Two-hybrid Homo sapiens
47 TTC19  
Two-hybrid Homo sapiens
48 GCC1 79571
Two-hybrid Homo sapiens
49 FAM9A  
Two-hybrid Homo sapiens
50 NDOR1  
Two-hybrid Homo sapiens
51 WDYHV1  
Two-hybrid Homo sapiens
52 TCHP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 PPARD  
Affinity Capture-MS Homo sapiens
54 DNAJB9 4189
Affinity Capture-MS Homo sapiens
55 TGIF2LY  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 CTNNA3  
Two-hybrid Homo sapiens
57 UTP14C  
Two-hybrid Homo sapiens
58 CEBPA  
Proximity Label-MS Homo sapiens
59 HSPB8 26353
Affinity Capture-MS Homo sapiens
60 UBXN6 80700
Affinity Capture-MS Homo sapiens
61 USP32 84669
Affinity Capture-MS Homo sapiens
62 CTAG1A  
Two-hybrid Homo sapiens
63 PSG11  
Affinity Capture-MS Homo sapiens
64 NOTCH2NL 388677
Two-hybrid Homo sapiens
65 DGCR6L  
Two-hybrid Homo sapiens
66 NBEA  
Affinity Capture-MS Homo sapiens
67 CCDC57 284001
Two-hybrid Homo sapiens
68 KRTAP10-8  
Two-hybrid Homo sapiens
69 PRKAA2 5563
Two-hybrid Homo sapiens
70 METTL21B  
Affinity Capture-MS Homo sapiens
71 MORF4L1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 VBP1 7411
Co-fractionation Homo sapiens
73 G6PD 2539
Co-fractionation Homo sapiens
74 CRYBB3  
Affinity Capture-MS Homo sapiens
75 C12orf74  
Affinity Capture-MS Homo sapiens
76 DNAI2  
Affinity Capture-MS Homo sapiens
77 TCEANC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 MGC50722  
Two-hybrid Homo sapiens
79 DGCR6  
Two-hybrid Homo sapiens
80 ZBED8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
81 SORBS3 10174
Two-hybrid Homo sapiens
82 SUMO1P1  
Two-hybrid Homo sapiens
83 PRKAB2 5565
Two-hybrid Homo sapiens
84 TMEM209 84928
Affinity Capture-MS Homo sapiens
85 ZNF417  
Two-hybrid Homo sapiens
86 TSGA10  
Two-hybrid Homo sapiens
87 GLS 2744
Affinity Capture-MS Homo sapiens
88 GRN 2896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 TRAF2 7186
Two-hybrid Homo sapiens
90 EXOC8 149371
Two-hybrid Homo sapiens
91 CCHCR1  
Two-hybrid Homo sapiens
92 PIN1 5300
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
93 CTAG1B  
Two-hybrid Homo sapiens
94 EIF5A2 56648
Affinity Capture-MS Homo sapiens
95 MORF4L2  
Two-hybrid Homo sapiens
96 CWF19L2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 CHD4 1108
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
98 PHOSPHO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
99 PSMA1 5682
Two-hybrid Homo sapiens
100 GSTM3 2947
Affinity Capture-MS Homo sapiens
101 DHFR 1719
Affinity Capture-MS Homo sapiens
102 DRG2 1819
Two-hybrid Homo sapiens
103 INF2 64423
Affinity Capture-MS Homo sapiens
104 JAKMIP2  
Two-hybrid Homo sapiens
105 TRIM23 373
Two-hybrid Homo sapiens
106 NAB2 4665
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
107 EID3  
Affinity Capture-MS Homo sapiens
108 CBX8 57332
Two-hybrid Homo sapiens
109 ANKRD11 29123
Two-hybrid Homo sapiens
110 ANK3  
Two-hybrid Homo sapiens
111 NAB1 4664
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 HSF2BP  
Two-hybrid Homo sapiens
113 POLD2 5425
Affinity Capture-MS Homo sapiens
114 GALNT9  
Affinity Capture-MS Homo sapiens
115 PIK3R3 8503
Two-hybrid Homo sapiens
View the network image/svg+xml



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