Gene description for POLD2
Gene name polymerase (DNA directed), delta 2, accessory subunit
Gene symbol POLD2
Other names/aliases -
Species Homo sapiens
 Database cross references - POLD2
ExoCarta ExoCarta_5425
Vesiclepedia VP_5425
Entrez Gene 5425
HGNC 9176
MIM 600815
UniProt P49005  
 POLD2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for POLD2
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    DNA-directed DNA polymerase activity GO:0003887 IEA
Biological Process
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    DNA replication GO:0006260 TAS
    nucleotide-excision repair GO:0006289 TAS
    mitotic cell cycle GO:0000278 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    base-excision repair GO:0006284 TAS
    mismatch repair GO:0006298 TAS
    translesion synthesis GO:0019985 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    telomere maintenance GO:0000723 TAS
    telomere maintenance via recombination GO:0000722 TAS
    DNA repair GO:0006281 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified POLD2 in exosomes
1
Experiment ID 234
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLDIP2 26073
Affinity Capture-MS Homo sapiens
2 LSM6 11157
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 POLDIP3 84271
Affinity Capture-MS Homo sapiens
4 KCTD13 253980
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
5 CDKN1A  
Affinity Capture-MS Homo sapiens
6 PCNA 5111
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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