Gene description for POLD1
Gene name polymerase (DNA directed), delta 1, catalytic subunit
Gene symbol POLD1
Other names/aliases CDC2
CRCS10
MDPL
POLD
Species Homo sapiens
 Database cross references - POLD1
ExoCarta ExoCarta_5424
Entrez Gene 5424
HGNC 9175
MIM 174761
UniProt P28340  
 POLD1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for POLD1
Molecular Function
    DNA binding GO:0003677 IDA
    DNA-directed DNA polymerase activity GO:0003887 IMP
    3'-5'-exodeoxyribonuclease activity GO:0008296 IBA
    chromatin binding GO:0003682 IDA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    damaged DNA binding GO:0003684 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    response to UV GO:0009411 TAS
    DNA-dependent DNA replication GO:0006261 IBA
    small molecule metabolic process GO:0044281 TAS
    DNA replication GO:0006260 IMP
    cellular response to UV GO:0034644 IDA
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    mitotic cell cycle GO:0000278 TAS
    telomere maintenance GO:0000723 TAS
    DNA synthesis involved in DNA repair GO:0000731 IMP
    nucleic acid phosphodiester bond hydrolysis GO:0090305 IEA
    fatty acid homeostasis GO:0055089 IMP
    mismatch repair GO:0006298 TAS
    DNA repair GO:0006281 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    nucleotide-excision repair GO:0006289 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    base-excision repair, gap-filling GO:0006287 IDA
    base-excision repair GO:0006284 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    translesion synthesis GO:0019985 TAS
    DNA replication proofreading GO:0045004 IBA
    telomere maintenance via recombination GO:0000722 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
    aggresome GO:0016235 IDA
    nucleotide-excision repair complex GO:0000109 IDA
    delta DNA polymerase complex GO:0043625 IBA
 Experiment description of studies that identified POLD1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Protein-peptide Homo sapiens
2 RFC1 5981
Invivo Homo sapiens
Invitro Homo sapiens
3 PCNA 5111
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
4 POLA  
Invivo Homo sapiens
Invitro Homo sapiens
5 CHGB  
Two-hybrid Homo sapiens
6 PTP4A3  
Affinity Capture-MS Homo sapiens
7 TREX2  
Invitro Homo sapiens
8 KIAA1377  
Two-hybrid Homo sapiens
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