Gene description for PCNA
Gene name proliferating cell nuclear antigen
Gene symbol PCNA
Other names/aliases ATLD2
Species Homo sapiens
 Database cross references - PCNA
ExoCarta ExoCarta_5111
Entrez Gene 5111
HGNC 8729
MIM 176740
UniProt P12004  
 PCNA identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PCNA
Molecular Function
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 IDA
    MutLalpha complex binding GO:0032405 IDA
    damaged DNA binding GO:0003684 IDA
    dinucleotide insertion or deletion binding GO:0032139 IDA
    identical protein binding GO:0042802 IPI
    histone acetyltransferase binding GO:0035035 IPI
    purine-specific mismatch base pair DNA N-glycosylase activity GO:0000701 IDA
    DNA polymerase processivity factor activity GO:0030337 IBA
    DNA polymerase binding GO:0070182 IPI
    receptor tyrosine kinase binding GO:0030971 IPI
Biological Process
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    cell proliferation GO:0008283 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    positive regulation of deoxyribonuclease activity GO:0032077 IDA
    telomere maintenance GO:0000723 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    mismatch repair GO:0006298 TAS
    error-prone translesion synthesis GO:0042276 TAS
    epithelial cell differentiation GO:0030855 IEP
    DNA damage response, detection of DNA damage GO:0042769 TAS
    regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0000083 TAS
    error-free translesion synthesis GO:0070987 TAS
    response to lipid GO:0033993 IEA
    telomere maintenance via recombination GO:0000722 TAS
    leading strand elongation GO:0006272 IBA
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    nucleotide-excision repair GO:0006289 TAS
    translesion synthesis GO:0019985 TAS
    DNA repair GO:0006281 TAS
    cellular response to UV GO:0034644 IDA
    response to cadmium ion GO:0046686 IEA
    positive regulation of DNA repair GO:0045739 IMP
    heart development GO:0007507 IEA
    mitotic cell cycle GO:0000278 TAS
    positive regulation of DNA replication GO:0045740 IMP
    base-excision repair GO:0006284 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    DNA replication factor C complex GO:0005663 TAS
    centrosome GO:0005813 IDA
    nuclear replication fork GO:0043596 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    PCNA complex GO:0043626 IDA
    extracellular exosome GO:0070062 IDA
    PCNA-p21 complex GO:0070557 IDA
 Experiment description of studies that identified PCNA in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PCNA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YBX1 4904
Invitro Homo sapiens
Invivo Homo sapiens
2 Gadd45b  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
3 PARP1 142
Affinity Capture-Western Homo sapiens
4 HUS1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 MSH2 4436
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 MSH6 2956
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
7 PCNA 5111
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
8 PTMA 5757
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
9 SUB1 10923
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 FEN1 2237
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 KCTD13  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
12 EXO1  
Affinity Capture-MS Homo sapiens
13 GADD45G  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
14 GADD45A  
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 POLD3  
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
16 CHTF18 63922
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
17 ERCC5  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 RPA1 6117
Invitro Homo sapiens
19 POLD1 5424
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 DCC1  
Affinity Capture-MS Homo sapiens
21 GADD45B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
22 UNG  
Affinity Capture-MS Homo sapiens
23 CDC6  
Two-hybrid Homo sapiens
24 RFC5 5985
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
25 RUVBL2 10856
Invitro Homo sapiens
26 CDKN1A  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
27 CDK2 1017
Invitro Homo sapiens
28 CDK5 1020
Invitro Homo sapiens
29 MCL1 4170
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
30 CDK6 1021
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
31 WRN  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
32 MSH3 4437
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
33 PRKDC 5591
Invitro Homo sapiens
34 CDK1 983
Invitro Homo sapiens
35 NONO 4841
Affinity Capture-MS Homo sapiens
36 RFC1 5981
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
37 MUTYH  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
38 HDAC1 3065
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 APEX1 328
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
40 RAD9A  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
41 POLH  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
42 CDC25C  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
43 CCNB1 891
Invivo Homo sapiens
44 ING1  
Invivo Homo sapiens
Invitro Homo sapiens
45 RFC2 5982
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
46 RFC3 5983
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
47 DNTT 1791
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
48 CDK4 1019
Affinity Capture-Western Homo sapiens
49 CHAF1A  
Invitro Homo sapiens
Two-hybrid Homo sapiens
50 POLDIP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
51 XRCC5 7520
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
52 UNG2  
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
53 LIG1 3978
Invitro Homo sapiens
54 CCNA2 890
Affinity Capture-Western Homo sapiens
55 XRCC6 2547
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
56 POLL  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
57 TCOF1 6949
Affinity Capture-MS Homo sapiens
58 RFC4 5984
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
59 DHX9 1660
Invitro Homo sapiens
60 XRCC1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
61 APEX2  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
62 ANXA2 302
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
63 POLE  
Invitro Homo sapiens
64 CCND1 595
Reconstituted Complex Homo sapiens
65 DNMT1 1786
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
66 MYBBP1A 10514
Invitro Homo sapiens
67 POLD2 5425
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
68 CDKN1C  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
69 EP300 2033
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
70 CCND3  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
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