Gene description for STK39
Gene name serine threonine kinase 39
Gene symbol STK39
Other names/aliases DCHT
PASK
SPAK
Species Homo sapiens
 Database cross references - STK39
ExoCarta ExoCarta_27347
Vesiclepedia VP_27347
Entrez Gene 27347
HGNC 17717
MIM 607648
UniProt Q9UEW8  
 STK39 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for STK39
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 ISS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    response to dietary excess GO:0002021 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISS
    cell volume homeostasis GO:0006884 IDA
    inflammatory response GO:0006954 IEA
    signal transduction GO:0007165 IDA
    signal transduction GO:0007165 IMP
    regulation of blood pressure GO:0008217 IEA
    positive regulation of T cell chemotaxis GO:0010820 IBA
    positive regulation of T cell chemotaxis GO:0010820 IMP
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 IMP
    peptidyl-threonine phosphorylation GO:0018107 IDA
    peptidyl-threonine phosphorylation GO:0018107 IMP
    peptidyl-threonine phosphorylation GO:0018107 ISS
    intracellular chloride ion homeostasis GO:0030644 IEA
    positive regulation of ion transmembrane transporter activity GO:0032414 ISS
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    sodium ion transmembrane transport GO:0035725 IEA
    cellular response to potassium ion GO:0035865 IEA
    maintenance of lens transparency GO:0036438 ISS
    chemokine (C-X-C motif) ligand 12 signaling pathway GO:0038146 IMP
    macrophage activation GO:0042116 IEA
    protein autophosphorylation GO:0046777 IMP
    protein autophosphorylation GO:0046777 ISS
    regulation of inflammatory response GO:0050727 IEA
    renal sodium ion absorption GO:0070294 IDA
    cellular hyperosmotic response GO:0071474 IBA
    cellular hyperosmotic response GO:0071474 IDA
    cellular hypotonic response GO:0071476 IDA
    negative regulation of pancreatic juice secretion GO:0090188 IEA
    positive regulation of p38MAPK cascade GO:1900745 IMP
    negative regulation of potassium ion transmembrane transporter activity GO:1901017 IDA
    negative regulation of potassium ion transmembrane transport GO:1901380 IDA
    response to aldosterone GO:1904044 IEA
    negative regulation of creatine transmembrane transporter activity GO:1905408 IDA
    cellular response to chemokine GO:1990869 IMP
    negative regulation of sodium ion transmembrane transporter activity GO:2000650 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IMP
    cytosol GO:0005829 ISS
    cell cortex GO:0005938 ISS
    basolateral plasma membrane GO:0016323 IEA
    apical plasma membrane GO:0016324 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    cell body GO:0044297 IEA
 Experiment description of studies that identified STK39 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STK39
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 GSN 2934
Two-hybrid Homo sapiens
3 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RELL1 768211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CSTL1  
Affinity Capture-MS Homo sapiens
6 WNK4  
Two-hybrid Homo sapiens
7 BAGE2  
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 AATK  
Two-hybrid Homo sapiens
10 TMEM132D  
Two-hybrid Homo sapiens
11 RAMP2  
Affinity Capture-MS Homo sapiens
12 EFNA4  
Affinity Capture-MS Homo sapiens
13 DEFB136  
Affinity Capture-MS Homo sapiens
14 LGI1  
Affinity Capture-MS Homo sapiens
15 PSG1  
Affinity Capture-MS Homo sapiens
16 EHD1 10938
Co-fractionation Homo sapiens
17 CRLF2 64109
Affinity Capture-MS Homo sapiens
18 PCNA 5111
Co-fractionation Homo sapiens
19 HCST  
Affinity Capture-MS Homo sapiens
20 GYPA  
Affinity Capture-MS Homo sapiens
21 SPP1 6696
Two-hybrid Homo sapiens
22 ATL2 64225
Affinity Capture-MS Homo sapiens
23 CST8  
Affinity Capture-MS Homo sapiens
24 STK39 27347
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
25 PML 5371
Affinity Capture-MS Homo sapiens
26 NOL3 8996
Co-fractionation Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 CDC25B 994
Affinity Capture-MS Homo sapiens
29 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 XPNPEP1 7511
Co-fractionation Homo sapiens
31 FKBP9 11328
Co-fractionation Homo sapiens
32 CHCHD4  
Affinity Capture-MS Homo sapiens
33 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 METTL21B  
Affinity Capture-MS Homo sapiens
35 SLC12A2 6558
Two-hybrid Homo sapiens
36 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
37 RNF7  
Affinity Capture-MS Homo sapiens
38 HEXA 3073
Co-fractionation Homo sapiens
39 TOMM22 56993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RELL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SLC18A2  
Affinity Capture-MS Homo sapiens
42 TSC22D4 81628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 API5 8539
Co-fractionation Homo sapiens
44 SLC12A6 9990
Affinity Capture-MS Homo sapiens
45 OSTM1 28962
Affinity Capture-MS Homo sapiens
46 IL12RB1  
Affinity Capture-MS Homo sapiens
47 SV2A 9900
Affinity Capture-MS Homo sapiens
48 RELT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MCM2 4171
Affinity Capture-MS Homo sapiens
50 KLK15  
Affinity Capture-MS Homo sapiens
51 PLK1 5347
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
52 BRD1 23774
Affinity Capture-MS Homo sapiens
53 NUBP1 4682
Co-fractionation Homo sapiens
54 TSC22D2 9819
Two-hybrid Homo sapiens
55 DHFRL1  
Affinity Capture-MS Homo sapiens
56 PRKY  
Affinity Capture-MS Homo sapiens
57 NUP210P1  
Affinity Capture-MS Homo sapiens
58 HSPH1 10808
Two-hybrid Homo sapiens
59 MAPK14 1432
Affinity Capture-Western Homo sapiens
60 BANF2  
Affinity Capture-MS Homo sapiens
61 SLC22A4 6583
Affinity Capture-MS Homo sapiens
62 RHOA 387
Affinity Capture-MS Homo sapiens
63 CCT8L2  
Affinity Capture-MS Homo sapiens
64 RPA3 6119
Proximity Label-MS Homo sapiens
65 ARX  
Affinity Capture-MS Homo sapiens
66 MBP 4155
Biochemical Activity Homo sapiens
67 EP300 2033
Affinity Capture-MS Homo sapiens
68 OTOF  
Two-hybrid Homo sapiens
69 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 HIF1A 3091
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which STK39 is involved
No pathways found





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