Gene description for MBP
Gene name myelin basic protein
Gene symbol MBP
Other names/aliases -
Species Homo sapiens
 Database cross references - MBP
ExoCarta ExoCarta_4155
Vesiclepedia VP_4155
Entrez Gene 4155
HGNC 6925
MIM 159430
UniProt P02686  
 MBP identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for MBP
Molecular Function
    protease binding GO:0002020 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IPI
    lipid binding GO:0008289 EXP
Biological Process
    MAPK cascade GO:0000165 IDA
    immune response GO:0006955 TAS
    chemical synaptic transmission GO:0007268 TAS
    central nervous system development GO:0007417 TAS
    sensory perception of sound GO:0007605 IEA
    axon ensheathment GO:0008366 TAS
    response to toxic substance GO:0009636 IEA
    substantia nigra development GO:0021762 HEP
    positive regulation of interleukin-6 production GO:0032755 IDA
    negative regulation of heterotypic cell-cell adhesion GO:0034115 IDA
    maintenance of blood-brain barrier GO:0035633 IDA
    myelination GO:0042552 IBA
    membrane organization GO:0061024 IEA
    positive regulation of metalloendopeptidase activity GO:1904685 IDA
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    cell surface GO:0009986 ISS
    protein-containing complex GO:0032991 IDA
    internode region of axon GO:0033269 IBA
    neuronal cell body GO:0043025 IBA
    myelin sheath GO:0043209 IDA
    compact myelin GO:0043218 IBA
    synapse GO:0045202 IEA
    cell periphery GO:0071944 IBA
 Experiment description of studies that identified MBP in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZER1 10444
Affinity Capture-MS Homo sapiens
2 DUSP22  
Affinity Capture-MS Homo sapiens
3 ATXN1 6310
Two-hybrid Homo sapiens
4 LIMK1 3984
Biochemical Activity Homo sapiens
5 STK3 6788
Biochemical Activity Homo sapiens
6 MUS81  
Synthetic Growth Defect Homo sapiens
7 NMT1 4836
Affinity Capture-MS Homo sapiens
8 TLK1  
Biochemical Activity Homo sapiens
9 PLP1 5354
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
10 CTDSP1 58190
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CDK9 1025
Biochemical Activity Homo sapiens
12 HLA-DRA 3122
Co-crystal Structure Homo sapiens
13 MAPK1 5594
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
14 TSSK6 83983
Biochemical Activity Homo sapiens
15 Tak1  
Biochemical Activity Drosophila melanogaster
16 ADRB2  
Two-hybrid Homo sapiens
17 IRAK4 51135
Biochemical Activity Homo sapiens
18 MAPK13 5603
Biochemical Activity Homo sapiens
19 STK39 27347
Biochemical Activity Homo sapiens
20 PRKCZ 5590
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
21 MAPK3 5595
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
22 PLEKHF2 79666
Two-hybrid Homo sapiens
23 Gtf3c2  
Affinity Capture-MS Mus musculus
24 Prkca  
Biochemical Activity Rattus norvegicus
25 ABL1 25
Biochemical Activity Homo sapiens
26 MAPK12  
Biochemical Activity Homo sapiens
27 MAP3K1 4214
Biochemical Activity Homo sapiens
28 MAP3K5 4217
Biochemical Activity Homo sapiens
29 CDK5 1020
Biochemical Activity Homo sapiens
30 PRKCB 5579
Biochemical Activity Homo sapiens
31 ATN1  
Two-hybrid Homo sapiens
32 DDX58 23586
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 PRKDC 5591
Biochemical Activity Homo sapiens
34 CDK1 983
Biochemical Activity Homo sapiens
35 Map3k5  
Biochemical Activity Mus musculus
36 PRKCI 5584
Biochemical Activity Homo sapiens
37 IDE 3416
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TBK1 29110
Biochemical Activity Homo sapiens
39 PRMT5 10419
Biochemical Activity Homo sapiens
40 NLN 57486
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CTDSP2 10106
Affinity Capture-MS Homo sapiens
42 CCR1  
Two-hybrid Homo sapiens
43 LRRK2 120892
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
44 PRKCA 5578
Biochemical Activity Homo sapiens
45 PPP2R5C 5527
Biochemical Activity Homo sapiens
46 TRPM7 54822
Biochemical Activity Homo sapiens
47 ULK1  
Biochemical Activity Homo sapiens
48 Map2k1 170851
Biochemical Activity Rattus norvegicus
49 MAPK14 1432
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
50 Prkaca 25636
Biochemical Activity Rattus norvegicus
51 APTX  
Two-hybrid Homo sapiens
52 MAP3K3 4215
Biochemical Activity Homo sapiens
53 MAPK8 5599
Biochemical Activity Homo sapiens
54 LIMK2 3985
Biochemical Activity Homo sapiens
55 IFNA21  
Affinity Capture-MS Homo sapiens
56 CTDSPL 10217
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 IKBKE 9641
Biochemical Activity Homo sapiens
58 KCNH5  
Affinity Capture-MS Homo sapiens
59 MYH6 4624
Affinity Capture-MS Homo sapiens
60 PTAFR  
Two-hybrid Homo sapiens
61 NEIL3  
Protein-RNA Homo sapiens
62 RPS6KA6 27330
Co-fractionation Homo sapiens
63 MAPK11  
Biochemical Activity Homo sapiens
View the network image/svg+xml



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