Gene description for IRAK4
Gene name interleukin-1 receptor-associated kinase 4
Gene symbol IRAK4
Other names/aliases IPD1
IRAK-4
NY-REN-64
REN64
Species Homo sapiens
 Database cross references - IRAK4
ExoCarta ExoCarta_51135
Vesiclepedia VP_51135
Entrez Gene 51135
HGNC 17967
MIM 606883
UniProt Q9NWZ3  
 IRAK4 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for IRAK4
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein serine/threonine kinase activity GO:0004674 EXP
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    interleukin-1 receptor binding GO:0005149 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 IDA
    protein kinase binding GO:0019901 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    toll-like receptor signaling pathway GO:0002224 TAS
    neutrophil mediated immunity GO:0002446 IMP
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    chromatin remodeling GO:0006338 IEA
    JNK cascade GO:0007254 IEA
    Toll signaling pathway GO:0008063 IBA
    cytokine-mediated signaling pathway GO:0019221 IBA
    lipopolysaccharide-mediated signaling pathway GO:0031663 IBA
    toll-like receptor 4 signaling pathway GO:0034142 IC
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    intracellular signal transduction GO:0035556 IBA
    interleukin-33-mediated signaling pathway GO:0038172 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    innate immune response GO:0045087 TAS
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    interleukin-1-mediated signaling pathway GO:0070498 IDA
    interleukin-1-mediated signaling pathway GO:0070498 TAS
    neutrophil migration GO:1990266 IMP
Subcellular Localization
    extracellular space GO:0005615 IEA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IC
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    endosome membrane GO:0010008 TAS
    extrinsic component of plasma membrane GO:0019897 IC
 Experiment description of studies that identified IRAK4 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IRAK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MECP2 4204
Affinity Capture-MS Homo sapiens
2 IRAK2  
Affinity Capture-MS Homo sapiens
3 PELI2 57161
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
4 IL1R1 3554
Affinity Capture-Western Homo sapiens
5 TRIM7  
Two-hybrid Homo sapiens
6 HIST1H1D 3007
Biochemical Activity Homo sapiens
7 IKBKG 8517
Reconstituted Complex Homo sapiens
8 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 SQSTM1 8878
Proximity Label-MS Homo sapiens
10 IRAK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
PCA Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
11 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 IL36RN  
Two-hybrid Homo sapiens
13 LTN1 26046
Co-fractionation Homo sapiens
14 HIST1H1T 3010
Biochemical Activity Homo sapiens
15 HIST1H1B 3009
Biochemical Activity Homo sapiens
16 SASH1 23328
Affinity Capture-Western Homo sapiens
17 PELI1 57162
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
18 MBP 4155
Biochemical Activity Homo sapiens
19 HIST1H1C 3006
Biochemical Activity Homo sapiens
20 MYD88 4615
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 HIST1H1E 3008
Biochemical Activity Homo sapiens
22 ARNT 405
Two-hybrid Homo sapiens
23 HIST1H1A 3024
Biochemical Activity Homo sapiens
24 KIF11 3832
Affinity Capture-MS Homo sapiens
25 ZNF597  
Two-hybrid Homo sapiens
26 PELI3  
Affinity Capture-Western Homo sapiens
27 EIF4EBP1  
Biochemical Activity Homo sapiens
28 TLR4  
Affinity Capture-Western Homo sapiens
29 IRAK3 11213
Affinity Capture-MS Homo sapiens
30 IL1RL1 9173
Affinity Capture-Western Homo sapiens
31 TIRAP  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
PCA Homo sapiens
32 TNFRSF13B  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which IRAK4 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Diseases associated with the TLR signaling cascade TAS Reactome
Diseases of Immune System TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interleukin-1 family signaling TAS Reactome
Interleukin-1 signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRAK4 deficiency (TLR2/4) TAS Reactome
IRAK4 deficiency (TLR5) TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Signal Transduction TAS Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling TAS Reactome





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