Gene description for IRAK3
Gene name interleukin-1 receptor-associated kinase 3
Gene symbol IRAK3
Other names/aliases ASRT5
IRAKM
Species Homo sapiens
 Database cross references - IRAK3
ExoCarta ExoCarta_11213
Vesiclepedia VP_11213
Entrez Gene 11213
HGNC 17020
MIM 604459
UniProt Q9Y616  
 IRAK3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IRAK3
Molecular Function
    protein homodimerization activity GO:0042803 IPI
    ATP binding GO:0005524 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    magnesium ion binding GO:0000287 IDA
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    negative regulation of MAP kinase activity GO:0043407 IMP
    negative regulation of innate immune response GO:0045824 ISS
    negative regulation of macrophage cytokine production GO:0010936 ISS
    response to peptidoglycan GO:0032494 ISS
    response to lipopolysaccharide GO:0032496 ISS
    negative regulation of protein catabolic process GO:0042177 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    positive regulation of macrophage tolerance induction GO:0010933 ISS
    negative regulation of interleukin-12 production GO:0032695 ISS
    protein autophosphorylation GO:0046777 IEA
    negative regulation of protein complex disassembly GO:0043242 IMP
    response to virus GO:0009615 IC
    interleukin-1-mediated signaling pathway GO:0070498 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    negative regulation of toll-like receptor signaling pathway GO:0034122 ISS
    negative regulation of interleukin-6 production GO:0032715 ISS
    negative regulation of cytokine-mediated signaling pathway GO:0001960 IC
    response to interleukin-1 GO:0070555 IMP
    intracellular signal transduction GO:0035556 IBA
    protein phosphorylation GO:0006468 IDA
    negative regulation of tumor necrosis factor production GO:0032720 IMP
    response to exogenous dsRNA GO:0043330 ISS
    regulation of protein complex disassembly GO:0043244 ISS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    cytokine-mediated signaling pathway GO:0019221 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified IRAK3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IRAK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYD88 4615
Affinity Capture-Western Homo sapiens
2 TRAF6 7189
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which IRAK3 is involved
PathwayEvidenceSource
Interleukin-1 signaling TAS Reactome
MyD88:Mal cascade initiated on plasma membrane TAS Reactome





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