Gene description for IRAK3
Gene name interleukin-1 receptor-associated kinase 3
Gene symbol IRAK3
Other names/aliases ASRT5
IRAKM
Species Homo sapiens
 Database cross references - IRAK3
ExoCarta ExoCarta_11213
Vesiclepedia VP_11213
Entrez Gene 11213
HGNC 17020
MIM 604459
UniProt Q9Y616  
 IRAK3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IRAK3
Molecular Function
    magnesium ion binding GO:0000287 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein kinase binding GO:0019901 IPI
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 IPI
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    positive regulation of cytokine production GO:0001819 IEA
    negative regulation of cytokine-mediated signaling pathway GO:0001960 IC
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    protein phosphorylation GO:0006468 IDA
    Toll signaling pathway GO:0008063 IBA
    response to virus GO:0009615 IC
    positive regulation of macrophage tolerance induction GO:0010933 ISS
    negative regulation of macrophage cytokine production GO:0010936 ISS
    cytokine-mediated signaling pathway GO:0019221 ISS
    lipopolysaccharide-mediated signaling pathway GO:0031663 IBA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    response to peptidoglycan GO:0032494 ISS
    response to lipopolysaccharide GO:0032496 ISS
    negative regulation of interleukin-12 production GO:0032695 IMP
    negative regulation of interleukin-12 production GO:0032695 ISS
    negative regulation of interleukin-6 production GO:0032715 IMP
    negative regulation of interleukin-6 production GO:0032715 ISS
    negative regulation of tumor necrosis factor production GO:0032720 IMP
    negative regulation of toll-like receptor signaling pathway GO:0034122 ISS
    intracellular signal transduction GO:0035556 IBA
    negative regulation of protein catabolic process GO:0042177 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IBA
    negative regulation of protein-containing complex disassembly GO:0043242 IMP
    regulation of protein-containing complex disassembly GO:0043244 ISS
    response to exogenous dsRNA GO:0043330 ISS
    negative regulation of MAP kinase activity GO:0043407 IMP
    negative regulation of innate immune response GO:0045824 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    interleukin-1-mediated signaling pathway GO:0070498 IBA
    interleukin-1-mediated signaling pathway GO:0070498 IMP
    response to interleukin-1 GO:0070555 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified IRAK3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IRAK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Two-hybrid Homo sapiens
2 RSL1D1 26156
Cross-Linking-MS (XL-MS) Homo sapiens
3 CD14 929
Two-hybrid Homo sapiens
4 GSDMD 79792
Affinity Capture-Western Homo sapiens
5 IRAK2  
Affinity Capture-MS Homo sapiens
6 FOLR1 2348
Two-hybrid Homo sapiens
7 MYD88 4615
Affinity Capture-Western Homo sapiens
8 IRAK4 51135
Affinity Capture-MS Homo sapiens
9 DPP8 54878
Affinity Capture-MS Homo sapiens
10 TRAF6 7189
Affinity Capture-Western Homo sapiens
11 NR3C1 2908
Proximity Label-MS Homo sapiens
12 HOXB7 3217
Two-hybrid Homo sapiens
13 IRAK1  
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 NSA2  
Two-hybrid Homo sapiens
15 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
16 NONO 4841
Two-hybrid Homo sapiens
17 COPB1 1315
Two-hybrid Homo sapiens
18 TBK1 29110
Affinity Capture-MS Homo sapiens
19 FKBP5 2289
Affinity Capture-MS Homo sapiens
20 NTRK3 4916
Two-hybrid Homo sapiens
21 LDB1  
Two-hybrid Homo sapiens
22 ADH1B 125
Two-hybrid Homo sapiens
23 ECM1 1893
Two-hybrid Homo sapiens
24 ATP6V0B 533
Two-hybrid Homo sapiens
25 CD44 960
Affinity Capture-MS Homo sapiens
26 HSPB8 26353
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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