Gene description for HSPB8
Gene name heat shock 22kDa protein 8
Gene symbol HSPB8
Other names/aliases CMT2L
DHMN2
E2IG1
H11
HMN2
HMN2A
HSP22
Species Homo sapiens
 Database cross references - HSPB8
ExoCarta ExoCarta_26353
Vesiclepedia VP_26353
Entrez Gene 26353
HGNC 30171
MIM 608014
UniProt Q9UJY1  
 HSPB8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Malignant ascites 12147373    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HSPB8
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
Biological Process
    cellular response to unfolded protein GO:0034620 IBA
    cellular response to unfolded protein GO:0034620 IMP
    positive regulation of aggrephagy GO:1905337 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    protein folding chaperone complex GO:0101031 IBA
    protein folding chaperone complex GO:0101031 IDA
 Experiment description of studies that identified HSPB8 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 56
MISEV standards
EM
Biophysical techniques
HSC70|MHCI|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12147373    
Organism Homo sapiens
Experiment description Malignant effusions and immunogenic tumour-derived exosomes.
Authors "Andre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L"
Journal name LANCET
Publication year 2002
Sample Malignant ascites
Sample name Malignant ascites
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HSPB8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABCF3 55324
Affinity Capture-MS Homo sapiens
2 OSBPL10  
Affinity Capture-MS Homo sapiens
3 HSPB7  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 WDR20 91833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ALMS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ATXN7L2  
Two-hybrid Homo sapiens
7 NRSN1  
Affinity Capture-MS Homo sapiens
8 PKP2 5318
Affinity Capture-MS Homo sapiens
9 AKAP11 11215
Affinity Capture-MS Homo sapiens
10 ANAPC5 51433
Affinity Capture-MS Homo sapiens
11 TUBB8 347688
Affinity Capture-MS Homo sapiens
12 IFT122 55764
Affinity Capture-MS Homo sapiens
13 TCF7L2  
Affinity Capture-MS Homo sapiens
14 MYO9B 4650
Affinity Capture-MS Homo sapiens
15 ZNF92  
Affinity Capture-MS Homo sapiens
16 RIPK1 8737
Affinity Capture-MS Homo sapiens
17 GTF2H2  
Affinity Capture-MS Homo sapiens
18 DNAJB6 10049
Affinity Capture-Western Homo sapiens
19 KIF20A 10112
Affinity Capture-MS Homo sapiens
20 DUSP12 11266
Two-hybrid Homo sapiens
21 PLEKHG4  
Affinity Capture-MS Homo sapiens
22 ETV3  
Affinity Capture-MS Homo sapiens
23 ERF  
Affinity Capture-MS Homo sapiens
24 DYRK1A 1859
Affinity Capture-MS Homo sapiens
25 APAF1 317
Affinity Capture-MS Homo sapiens
26 CIAPIN1 57019
Co-fractionation Homo sapiens
27 KLHDC10 23008
Affinity Capture-MS Homo sapiens
28 CASC5  
Affinity Capture-MS Homo sapiens
29 DLGAP4 22839
Affinity Capture-MS Homo sapiens
30 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
31 CAMK2B 816
Affinity Capture-MS Homo sapiens
32 ASB4  
Affinity Capture-MS Homo sapiens
33 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
34 VPS16 64601
Affinity Capture-MS Homo sapiens
35 ARFGEF2 10564
Affinity Capture-MS Homo sapiens
36 NPAS1  
Affinity Capture-MS Homo sapiens
37 VWA8 23078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 IFT140 9742
Affinity Capture-MS Homo sapiens
39 KCTD13 253980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 LONRF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 Mcm2 17216
Affinity Capture-MS Mus musculus
42 CPNE2 221184
Affinity Capture-MS Homo sapiens
43 POLD1 5424
Affinity Capture-MS Homo sapiens
44 FOXA1  
Affinity Capture-MS Homo sapiens
45 USP12 219333
Affinity Capture-MS Homo sapiens
46 RFX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KLHL34  
Affinity Capture-MS Homo sapiens
48 SOCS7  
Two-hybrid Homo sapiens
49 TUBA1A 7846
Affinity Capture-MS Homo sapiens
50 BRCA2 675
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 EXPH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 FOXK2  
Affinity Capture-MS Homo sapiens
53 RIN1 9610
Affinity Capture-MS Homo sapiens
54 DCAF10  
Affinity Capture-MS Homo sapiens
55 KIF11 3832
Affinity Capture-MS Homo sapiens
56 PML 5371
Affinity Capture-MS Homo sapiens
57 ARAP3 64411
Affinity Capture-MS Homo sapiens
58 POLE  
Affinity Capture-MS Homo sapiens
59 HSPB3  
Two-hybrid Homo sapiens
60 HSPB8 26353
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 UBXN6 80700
Affinity Capture-MS Homo sapiens
62 RPL11 6135
Affinity Capture-MS Homo sapiens
63 STUB1 10273
Co-localization Homo sapiens
64 BAG3 9531
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 HELZ 9931
Affinity Capture-MS Homo sapiens
66 TONSL  
Affinity Capture-MS Homo sapiens
67 ASCC3 10973
Affinity Capture-MS Homo sapiens
68 IQSEC1 9922
Affinity Capture-MS Homo sapiens
69 KIAA0100  
Affinity Capture-MS Homo sapiens
70 NUDT12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TAX1BP1 8887
Co-localization Homo sapiens
72 XPO1 7514
Affinity Capture-MS Homo sapiens
73 CRYAB 1410
Two-hybrid Homo sapiens
FRET Homo sapiens
74 HERC2P9  
Affinity Capture-MS Homo sapiens
75 ACAD11 84129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 Ttll12 223723
Affinity Capture-MS Mus musculus
77 SETD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 XAB2 56949
Affinity Capture-MS Homo sapiens
79 FRMD5 84978
Affinity Capture-MS Homo sapiens
80 AURKB 9212
Affinity Capture-MS Homo sapiens
81 MLF1  
Affinity Capture-MS Homo sapiens
82 HAUS5 23354
Affinity Capture-MS Homo sapiens
83 DUSP4 1846
Affinity Capture-MS Homo sapiens
84 HSPB6 126393
Two-hybrid Homo sapiens
FRET Homo sapiens
85 JAK1 3716
Affinity Capture-MS Homo sapiens
86 RGPD5  
Affinity Capture-MS Homo sapiens
87 SAV1  
Affinity Capture-MS Homo sapiens
88 AHR 196
Affinity Capture-MS Homo sapiens
89 EHMT2 10919
Affinity Capture-MS Homo sapiens
90 HSPA1A 3303
Co-localization Homo sapiens
91 EEF1A2 1917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 RICTOR 253260
Affinity Capture-MS Homo sapiens
93 ESRP2 80004
Affinity Capture-MS Homo sapiens
94 DSTN 11034
Co-fractionation Homo sapiens
95 ZER1 10444
Affinity Capture-MS Homo sapiens
96 TRIB1 10221
Affinity Capture-MS Homo sapiens
97 RPIA 22934
Two-hybrid Homo sapiens
98 IRAK3 11213
Affinity Capture-MS Homo sapiens
99 ALS2CR11  
Two-hybrid Homo sapiens
100 DNAJC13 23317
Affinity Capture-MS Homo sapiens
101 Smchd1  
Affinity Capture-MS Mus musculus
102 RGL3  
Affinity Capture-MS Homo sapiens
103 SNCA 6622
Reconstituted Complex Homo sapiens
104 PDLIM7 9260
Affinity Capture-MS Homo sapiens
105 SUN1 23353
Affinity Capture-MS Homo sapiens
106 HSPB2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
107 RHBDF2 79651
Affinity Capture-MS Homo sapiens
108 USP34 9736
Affinity Capture-MS Homo sapiens
109 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
110 RGPD8  
Affinity Capture-MS Homo sapiens
111 CHTF18 63922
Affinity Capture-MS Homo sapiens
112 RPTOR 57521
Affinity Capture-MS Homo sapiens
113 DNAAF5 54919
Affinity Capture-MS Homo sapiens
114 SQSTM1 8878
Co-localization Homo sapiens
115 Timeless  
Affinity Capture-MS Mus musculus
116 HSPB1 3315
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
117 TBC1D2B 23102
Affinity Capture-MS Homo sapiens
118 FAM83H 286077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 VPS33A 65082
Affinity Capture-MS Homo sapiens
120 ZNF195  
Affinity Capture-MS Homo sapiens
121 TFCP2L1  
Affinity Capture-MS Homo sapiens
122 QRICH1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
123 PMVK 10654
Affinity Capture-MS Homo sapiens
124 NAB2 4665
Affinity Capture-MS Homo sapiens
125 NACC2  
Affinity Capture-MS Homo sapiens
126 KANK2 25959
Affinity Capture-MS Homo sapiens
127 SLC16A5  
Two-hybrid Homo sapiens
128 ZBTB20 26137
Affinity Capture-MS Homo sapiens
129 CCR1  
Affinity Capture-MS Homo sapiens
130 CDK11A 728642
Affinity Capture-MS Homo sapiens
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