Gene description for ARHGEF10
Gene name Rho guanine nucleotide exchange factor (GEF) 10
Gene symbol ARHGEF10
Other names/aliases GEF10
SNCV
Species Homo sapiens
 Database cross references - ARHGEF10
ExoCarta ExoCarta_9639
Vesiclepedia VP_9639
Entrez Gene 9639
HGNC 14103
MIM 608136
UniProt O15013  
 ARHGEF10 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGEF10
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
    kinesin binding GO:0019894 IPI
Biological Process
    myelination in peripheral nervous system GO:0022011 IMP
    actin cytoskeleton organization GO:0030036 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    centrosome duplication GO:0051298 IMP
    positive regulation of stress fiber assembly GO:0051496 IBA
    positive regulation of stress fiber assembly GO:0051496 IMP
    mitotic spindle assembly GO:0090307 IBA
    mitotic spindle assembly GO:0090307 IMP
    activation of GTPase activity GO:0090630 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGEF10 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGEF10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 SUGT1 10910
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 RAB35 11021
Proximity Label-MS Homo sapiens
5 ARHGAP31 57514
Affinity Capture-MS Homo sapiens
6 MICB  
Affinity Capture-MS Homo sapiens
7 LAMTOR1 55004
Proximity Label-MS Homo sapiens
8 ICAM1 3383
Affinity Capture-MS Homo sapiens
9 HSPA8 3312
Affinity Capture-MS Homo sapiens
10 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
11 GJA1 2697
Proximity Label-MS Homo sapiens
12 PRDX3 10935
Affinity Capture-MS Homo sapiens
13 BAG5 9529
Affinity Capture-MS Homo sapiens
14 RNF31 55072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CRK 1398
Affinity Capture-MS Homo sapiens
16 ARHGEF10L 55160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MEX3A  
Affinity Capture-RNA Homo sapiens
18 RSPRY1 89970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CD2AP 23607
Co-fractionation Homo sapiens
20 PAICS 10606
Affinity Capture-MS Homo sapiens
21 SETD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SPSB4  
Affinity Capture-MS Homo sapiens
23 BAG2 9532
Affinity Capture-MS Homo sapiens
24 FHL3 2275
Two-hybrid Homo sapiens
25 DCTN2 10540
Proximity Label-MS Homo sapiens
26 SSBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 HOOK3 84376
Affinity Capture-MS Homo sapiens
28 CXADR 1525
Proximity Label-MS Homo sapiens
29 SPSB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CASP14 23581
Affinity Capture-MS Homo sapiens
31 COPG1 22820
Co-fractionation Homo sapiens
32 S100A8 6279
Affinity Capture-MS Homo sapiens
33 CDC42 998
FRET Homo sapiens
34 SART3 9733
Co-fractionation Homo sapiens
35 CBWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 PTRH2 51651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 EPHA2 1969
Proximity Label-MS Homo sapiens
38 WDR48 57599
Affinity Capture-MS Homo sapiens
39 INF2 64423
Affinity Capture-MS Homo sapiens
40 C12orf74  
Affinity Capture-MS Homo sapiens
41 GGH 8836
Affinity Capture-MS Homo sapiens
42 RAB11A 8766
Proximity Label-MS Homo sapiens
43 RFXANK  
Affinity Capture-MS Homo sapiens
44 METTL21B  
Affinity Capture-MS Homo sapiens
45 RAB3B 5865
Proximity Label-MS Homo sapiens
46 FBXW7  
Affinity Capture-MS Homo sapiens
47 DHFRL1  
Proximity Label-MS Homo sapiens
48 STX7 8417
Proximity Label-MS Homo sapiens
49 PIK3R1 5295
Affinity Capture-MS Homo sapiens
50 HECTD1 25831
Affinity Capture-MS Homo sapiens
51 RAC1 5879
FRET Homo sapiens
52 CCDC88A 55704
Affinity Capture-MS Homo sapiens
53 HSPA12A 259217
Affinity Capture-MS Homo sapiens
54 RHOA 387
FRET Homo sapiens
55 DNAJB1 3337
Affinity Capture-MS Homo sapiens
56 DNAJB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 STIP1 10963
Affinity Capture-MS Homo sapiens
58 SF3B3 23450
Affinity Capture-MS Homo sapiens
59 HSPB8 26353
Affinity Capture-MS Homo sapiens
60 DNAJB11 51726
Affinity Capture-MS Homo sapiens
61 STX6 10228
Proximity Label-MS Homo sapiens
62 UBXN6 80700
Affinity Capture-MS Homo sapiens
63 HSPA12B 116835
Proximity Label-MS Homo sapiens
64 PSME4 23198
Co-fractionation Homo sapiens
65 KLHL14 57565
Affinity Capture-MS Homo sapiens
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