Gene description for RNF31
Gene name ring finger protein 31
Gene symbol RNF31
Other names/aliases HOIP
ZIBRA
Species Homo sapiens
 Database cross references - RNF31
ExoCarta ExoCarta_55072
Vesiclepedia VP_55072
Entrez Gene 55072
HGNC 16031
MIM 612487
UniProt Q96EP0  
 RNF31 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RNF31
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    K48-linked polyubiquitin modification-dependent protein binding GO:0036435 IBA
    identical protein binding GO:0042802 IPI
    ubiquitin binding GO:0043130 IDA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IBA
    linear polyubiquitin binding GO:1990450 IBA
Biological Process
    protein polyubiquitination GO:0000209 IDA
    CD40 signaling pathway GO:0023035 ISS
    defense response to bacterium GO:0042742 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    T cell receptor signaling pathway GO:0050852 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    negative regulation of necroptotic process GO:0060546 IEA
    protein linear polyubiquitination GO:0097039 IBA
    protein linear polyubiquitination GO:0097039 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
    positive regulation of xenophagy GO:1904417 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasmic side of plasma membrane GO:0009898 ISS
    CD40 receptor complex GO:0035631 ISS
    LUBAC complex GO:0071797 IDA
    LUBAC complex GO:0071797 IPI
 Experiment description of studies that identified RNF31 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RNF31
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CDC34 997
Reconstituted Complex Homo sapiens
3 NPRL2  
Affinity Capture-MS Homo sapiens
4 CCDC24  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 PRIM2 5558
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 AES 166
Two-hybrid Homo sapiens
7 TRIM35 23087
Affinity Capture-MS Homo sapiens
8 TRIM33 51592
Affinity Capture-MS Homo sapiens
9 FAM189B  
Affinity Capture-MS Homo sapiens
10 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
11 ENDOV  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LOXL4 84171
Affinity Capture-MS Homo sapiens
13 RIPK1 8737
Affinity Capture-Western Homo sapiens
14 ATN1  
Two-hybrid Homo sapiens
15 AKAP8L  
Affinity Capture-MS Homo sapiens
16 ATG13 9776
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
17 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 UBE2E2  
Reconstituted Complex Homo sapiens
19 TLR3  
Affinity Capture-Western Homo sapiens
20 UBE2K 3093
Reconstituted Complex Homo sapiens
21 D2HGDH 728294
Affinity Capture-MS Homo sapiens
22 KRTAP9-2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 CCDC14  
Affinity Capture-MS Homo sapiens
24 MAGOHB 55110
Affinity Capture-MS Homo sapiens
25 PRIM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SNRPC 6631
Two-hybrid Homo sapiens
27 CALCOCO2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FOXD4L6  
Affinity Capture-MS Homo sapiens
30 UBE2Q1 55585
Reconstituted Complex Homo sapiens
31 RIPK2  
Affinity Capture-Western Homo sapiens
32 UBE2W  
Reconstituted Complex Homo sapiens
33 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 INTS12  
Affinity Capture-MS Homo sapiens
35 SNX27 81609
Affinity Capture-Western Homo sapiens
36 NPAS1  
Affinity Capture-MS Homo sapiens
37 PLCB3 5331
Affinity Capture-MS Homo sapiens
38 TNFRSF1B  
Affinity Capture-Western Homo sapiens
39 FKBPL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 C9orf163  
Affinity Capture-MS Homo sapiens
41 IQCF2  
Affinity Capture-MS Homo sapiens
42 GFI1B  
Two-hybrid Homo sapiens
43 TNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SNTG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RABGEF1 27342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 UBE2E3 10477
Reconstituted Complex Homo sapiens
48 FAM96A  
Affinity Capture-MS Homo sapiens
49 CHMP1A 5119
Affinity Capture-MS Homo sapiens
50 ZNF444  
Affinity Capture-MS Homo sapiens
51 PLBD1 79887
Affinity Capture-MS Homo sapiens
52 UBE2T  
Reconstituted Complex Homo sapiens
53 IRAK1  
Affinity Capture-Western Homo sapiens
54 UBE2D4  
Reconstituted Complex Homo sapiens
55 B4GALT2  
Affinity Capture-MS Homo sapiens
56 VRK1 7443
Affinity Capture-MS Homo sapiens
57 BTF3L4 91408
Affinity Capture-MS Homo sapiens
58 NDUFA8 4702
Affinity Capture-MS Homo sapiens
59 NOD2 64127
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 SOCS3 9021
Two-hybrid Homo sapiens
61 IRF7  
Affinity Capture-Western Homo sapiens
62 AZI2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
63 KIAA1683  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FYN 2534
Affinity Capture-MS Homo sapiens
65 VCP 7415
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
66 NTRK1 4914
Affinity Capture-MS Homo sapiens
67 SMAD3 4088
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
68 PPARD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SPATA2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
70 MALT1 10892
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 UBE2D3 7323
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
72 CYLD  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
73 UBXN6 80700
Affinity Capture-MS Homo sapiens
74 LIPF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PSG11  
Affinity Capture-MS Homo sapiens
76 ATXN1 6310
Two-hybrid Homo sapiens
77 BIRC2  
Affinity Capture-MS Homo sapiens
78 CD40 958
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
79 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 ARRDC4  
Affinity Capture-MS Homo sapiens
81 Sharpin  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
82 NR0B2  
Two-hybrid Homo sapiens
83 NOS3  
Two-hybrid Homo sapiens
84 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
85 Ikbkg  
Co-crystal Structure Mus musculus
86 CARTPT  
Affinity Capture-MS Homo sapiens
87 C19orf66  
Affinity Capture-MS Homo sapiens
88 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
89 CUL3 8452
Affinity Capture-MS Homo sapiens
90 CARD11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
91 UBE2E1  
Reconstituted Complex Homo sapiens
92 CTAG2  
Affinity Capture-MS Homo sapiens
93 TSSC4  
Two-hybrid Homo sapiens
94 KIF1A 547
Two-hybrid Homo sapiens
95 UBE2R2 54926
Reconstituted Complex Homo sapiens
96 UBE2D2 7322
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
97 METTL21B  
Affinity Capture-MS Homo sapiens
98 HSPA1A 3303
Affinity Capture-MS Homo sapiens
99 OTULIN 90268
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
100 SF3B3 23450
Affinity Capture-MS Homo sapiens
101 STK4 6789
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
102 DUSP16  
Affinity Capture-MS Homo sapiens
103 METTL15  
Affinity Capture-MS Homo sapiens
104 UBE2D1 7321
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
105 PDLIM7 9260
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
106 NR0B1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
107 ZNF785  
Affinity Capture-MS Homo sapiens
108 TRAF2 7186
Affinity Capture-MS Homo sapiens
109 SWSAP1  
Affinity Capture-MS Homo sapiens
110 UBE2O 63893
Reconstituted Complex Homo sapiens
111 WRNIP1 56897
Affinity Capture-MS Homo sapiens
112 GBP1 2633
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 UQCC1 55245
Affinity Capture-MS Homo sapiens
114 EIF1AX 1964
Affinity Capture-MS Homo sapiens
115 RNF31 55072
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
116 ATXN7  
Two-hybrid Homo sapiens
117 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
118 AJUBA  
Affinity Capture-MS Homo sapiens
119 PITX1  
Two-hybrid Homo sapiens
120 UBE2C 11065
Reconstituted Complex Homo sapiens
121 BCL10  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
122 DDX58 23586
Affinity Capture-Western Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-Western Homo sapiens
123 TRIM25 7706
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
124 ESR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
125 UBB 7314
Affinity Capture-MS Homo sapiens
126 EIF3J 8669
Affinity Capture-MS Homo sapiens
127 INF2 64423
Affinity Capture-MS Homo sapiens
128 PRKCA 5578
Biochemical Activity Homo sapiens
129 SHARPIN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
130 UBE2Q2 92912
Reconstituted Complex Homo sapiens
131 AR 367
Affinity Capture-MS Homo sapiens
132 PRKCB 5579
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
133 VASN 114990
Two-hybrid Homo sapiens
134 RBCK1  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
135 GALNT9  
Affinity Capture-MS Homo sapiens
136 TRAF1 7185
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
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