Gene description for GBP1
Gene name guanylate binding protein 1, interferon-inducible
Gene symbol GBP1
Other names/aliases -
Species Homo sapiens
 Database cross references - GBP1
ExoCarta ExoCarta_2633
Vesiclepedia VP_2633
Entrez Gene 2633
HGNC 4182
MIM 600411
UniProt P32455  
 GBP1 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for GBP1
Molecular Function
    lipopolysaccharide binding GO:0001530 IDA
    actin binding GO:0003779 IDA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    G protein activity GO:0003925 IDA
    GDP phosphatase activity GO:0004382 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GDP binding GO:0019003 IDA
    enzyme binding GO:0019899 IPI
    cytokine binding GO:0019955 IPI
    spectrin binding GO:0030507 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    Hsp90 protein binding GO:0051879 IPI
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    negative regulation of interleukin-2 production GO:0032703 IMP
    defense response to bacterium GO:0042742 IDA
    defense response to bacterium GO:0042742 ISS
    defense response to protozoan GO:0042832 IDA
    innate immune response GO:0045087 IDA
    regulation of calcium-mediated signaling GO:0050848 IMP
    negative regulation of T cell receptor signaling pathway GO:0050860 IMP
    defense response to virus GO:0051607 IEA
    cytolysis in another organism GO:0051715 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    cellular response to type II interferon GO:0071346 IBA
    cellular response to type II interferon GO:0071346 IDA
    cellular response to interleukin-1 GO:0071347 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
    protein localization to vacuole GO:0072665 IDA
    positive regulation of pyroptotic inflammatory response GO:0140639 IDA
    non-canonical inflammasome complex assembly GO:0160075 IDA
    negative regulation of substrate adhesion-dependent cell spreading GO:1900025 IMP
    regulation of protein localization to plasma membrane GO:1903076 IGI
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    vesicle membrane GO:0012506 IDA
    vesicle membrane GO:0012506 IDA
    actin cytoskeleton GO:0015629 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 IDA
    symbiont cell surface GO:0106139 IDA
 Experiment description of studies that identified GBP1 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GCFC2  
Affinity Capture-MS Homo sapiens
2 AFM 173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 FAHD2B  
Affinity Capture-MS Homo sapiens
4 MAPKAPK2 9261
Co-fractionation Homo sapiens
5 NPEPPS 9520
Co-fractionation Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 GBP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ATG5 9474
Proximity Label-MS Homo sapiens
9 KCTD10 83892
Affinity Capture-MS Homo sapiens
10 STK38 11329
Co-fractionation Homo sapiens
11 SP1  
Affinity Capture-MS Homo sapiens
12 FERMT2 10979
Co-fractionation Homo sapiens
13 TYW3  
Affinity Capture-MS Homo sapiens
14 CDK5 1020
Co-fractionation Homo sapiens
15 GBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PPP1CC 5501
Co-fractionation Homo sapiens
18 DTD2 112487
Co-fractionation Homo sapiens
19 ZNF317 57693
Affinity Capture-MS Homo sapiens
20 MBNL1 4154
Affinity Capture-MS Homo sapiens
21 SSUH2  
Affinity Capture-MS Homo sapiens
22 GAPDH 2597
Co-fractionation Homo sapiens
23 RNF31 55072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GNB2 2783
Affinity Capture-MS Homo sapiens
25 GBP5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 U2AF2 11338
Affinity Capture-MS Homo sapiens
27 METAP2 10988
Co-fractionation Homo sapiens
28 TKFC 26007
Co-fractionation Homo sapiens
29 CTNNA3  
Affinity Capture-MS Homo sapiens
30 UTP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 DNAJB1 3337
Cross-Linking-MS (XL-MS) Homo sapiens
32 CAB39L 81617
Affinity Capture-MS Homo sapiens
33 ATP5B 506
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which GBP1 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome





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